Effect of human serum on inhibition of growth ofStaphylococcus aureus by antimicrobial agents

Stratton, Charles W.; Evans, Martin E.; Burch, Daniel J.; Hawley, H. Bradford; Horsman, Thomas A.; Tu, Kevin K.; Reller, L. Barth

doi:10.1007/bf02017797

Cited by 5 publications

(4 citation statements)

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“…It must be remembered that the SBT combines in theory only the results of determinations of the antimicrobial agent level in serum and of the MBC. Serum bactericidal activity has been shown to correlate with antimicrobial agent levels and MBCs (48,91). In addition, the killing rate of serum has been compared with the serum bactericidal activity in several studies (105)(106)(107).…”

Section: Stratton Clinical Relevance Of the Sbtmentioning

confidence: 99%

“…Among the variables in either human serum or broth known to affect the bactericidal activity of certain antimicrobial agents and microorganisms are proteins (4,24,52,78,79), pH (109), phosphates (51), osmolality and salt concentrations (51), and divalent cations (23). The use of human serum as the diluent in the SBT has been shown to be important for certain antimicrobial agents and microorganisms (10,48,67,91). Human serum, however, also has disadvantages including instability of pH (9), risk of transmission of hepatitis B virus or human immunodeficiency virus, inherent antibacterial activity (16,32), cost and lack pf availability, poor support of growth in comparison with broth media (88,91), and irreversible binding or increased degradation for some antimicrobial agents (61).…”

Section: Historical Review Of the Sbtmentioning

confidence: 99%

“…The use of human serum as the diluent in the SBT has been shown to be important for certain antimicrobial agents and microorganisms (10,48,67,91). Human serum, however, also has disadvantages including instability of pH (9), risk of transmission of hepatitis B virus or human immunodeficiency virus, inherent antibacterial activity (16,32), cost and lack pf availability, poor support of growth in comparison with broth media (88,91), and irreversible binding or increased degradation for some antimicrobial agents (61). Nevertheless, when the bactericidal activity of an antibiotic in human serum is being tested, a reasonable and logical diluent to use is human serum.…”

Section: Historical Review Of the Sbtmentioning

confidence: 99%

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Serum bactericidal test

Stratton

1988

Clin Microbiol Rev

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The serum bactericidal test represents one of the few in vitro tests performed in the clinical microbiology laboratory that combines the interaction of the pathogen, the antimicrobial agent, and the patient. Although the use of such a test antedates the antimicrobial era, its performance, results, and interpretation have been subject to question and controversy. Much of the confusion concerning the serum bactericidal test can be avoided by an understanding of the various factors which influence bactericidal testing. In addition, the methodologic aspects of the serum bactericidal test have recently been addressed and should place this test on firmer ground. New information on the clinical utility of this test is becoming available; additional data are needed to establish more clearly the usefulness of the serum bactericidal test in specific infections. Such clinical trials from multiple centers will enable firmer recommendations for the future use of the serum bactericidal test.

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