Effect of Human Resource Development on Livestock Production in District Chitral, Khyber Pakhtunkhwa, Pakistan(A Study Conducted by Agha Khan Rural Support Programme)

Safirullah,; Anwar, Khurshaid; Sajid, Muhammad Sohail; Abdur-Raziq,; Mujtaba, Muhammad; Kalim, Khisrao; Khan, Sartaj; Anwar, Ali; Ullah, T. I.

doi:10.3923/pjn.2013.821.826

Cited by 1 publication

(3 citation statements)

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“…Molecular detection rates were lower in this study as compared to previously reported serological studies in animals (2,20) because the present study explored blood samples for the extraction of leptospiral DNA, where bacteria are found only after 3-10 d exposure. This septicaemic phase is followed by an immune phase (4-30 d) which is characterised by an increased titre of antibodies correlated with the elimination of bacteria from blood (5,17,18).…”

Section: Discussioncontrasting

confidence: 56%

“…Its clinical signs are nonspecific, making its diagnosis a challenge (29). Despite high rates of seropositivity among animals in Pakistan (2,20), to date, there have been no data on the molecular studies of leptospirosis in horses or in any mammalian host. This investigation reports the first molecular evidence of equine leptospirosis in Pakistan, where previous serological reports have revealed a prevalence of over 40% (21).…”

Section: Discussionmentioning

confidence: 99%

“…All previous studies reporting Leptospira in Pakistan were based on serological testing (2,20). However, no attempts had ever been made at molecular detection of the organism in any mammalian host.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterisation of Leptospira strains in Pakistan

Sohail

Khan

Avais

et al. 2016

Journal of Veterinary Research

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Introduction: Leptospirosis affects a wide range of mammals, humans, and even a few poikilothermic animal species. In Pakistan, serological studies of equine leptospirosis have reported a prevalence of over 40%, but no study has ever been conducted towards molecular detection of Leptospira in horses. Material and Methods: Blood samples from 128 horses were screened using ELISA and 41 positive samples were examined for the presence of leptospiral DNA using specific primers for 16S rRNA gene. Results: Out of 41 tested samples, 20 samples were found to be PCR-positive, revealing a fragment of 306 bp after gel electrophoresis. Sequencing and phylogenetic analysis of positive samples revealed circulation of pathogenic Leptospira spp. in Pakistani horses. No evidence of circulation of intermediate species was found in this study. Conclusion: This study reports the first molecular evidence of equine leptospirosis in Pakistan and lays ground for further research in this area. It also confirms the efficiency of 16S rRNA for the diagnosis of equine leptospirosis.

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Section: Discussioncontrasting

confidence: 56%