Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity

Li, Shengnan; Guan, Xiuchen; Yu, Wenting; Zhao, Zeqing; Sun, Yaxi; Bai, Yuxing

doi:10.1111/odi.14671

Cited by 3 publications

(2 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study investigated the development of an in-situ tissue engineering scaffold (iTE-scaffold) using the coaxial electrospinning technique to sequentially deliver bFGF and BMP-2 [65][66][67][68]. The iTE-scaffold was found to effectively enhance the angiogenesis of periodontal ligament stem cells (PDLSCs) and induce macrophage polarization into the prohealing M2 phenotype, thereby modulating inflammation [7].…”

Section: Novel Findings In Scaffolds Incorporating Growth Factors For...mentioning

confidence: 99%

“…Regenerative medicine encompasses the substitution or rejuvenation of human cells, tissues, or organs. Langer and Vacanti propose that tissue engineering comprises a triad, which includes stem/progenitor cells, scaffolds supporting cell growth, and essential growth factors [65][66][67]. Termed the paracrine effect, stem cells demonstrate an exceptional ability to release cytokines locally, thus promoting regenerative processes and influencing the balance between tissue regeneration and degeneration.…”

Section: Cell-based Tissue Engineeringmentioning

confidence: 99%

See 1 more Smart Citation

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Angjelova,

Jovanova,

Polizzi

et al. 2024

Medicina

View full text Add to dashboard Cite

The regeneration of periodontal bone defects continues to be an essential therapeutic concern in dental biomaterials. Numerous biomaterials have been utilized in this sector so far. However, the immune response and vascularity in defect regions may be disregarded when evaluating the effectiveness of biomaterials for bone repair. Among several regenerative treatments, the most recent technique of in situ tissue engineering stands out for its ability to replicate endogenous restorative processes by combining scaffold with particular growth factors. Regenerative medicine solutions that combine biomaterials/scaffolds, cells, and bioactive substances have attracted significant interest, particularly for bone repair and regeneration. Dental stem cells (DSCs) share the same progenitor and immunomodulatory properties as other types of MSCs, and because they are easily isolable, they are regarded as desirable therapeutic agents in regenerative dentistry. Recent research has demonstrated that DSCs sown on newly designed synthetic bio-material scaffolds preserve their proliferative capacity while exhibiting increased differentiation and immuno-suppressive capabilities. As researchers discovered how short peptide sequences modify the adhesion and proliferative capacities of scaffolds by activating or inhibiting conventional osteogenic pathways, the scaffolds became more effective at priming MSCs. In this review, the many components of tissue engineering applied to bone engineering will be examined, and the impact of biomaterials on periodontal regeneration and bone cellular biology/molecular genetics will be addressed and updated.

show abstract

Section: Novel Findings In Scaffolds Incorporating Growth Factors For...mentioning

confidence: 99%

Section: Cell-based Tissue Engineeringmentioning

confidence: 99%

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Angjelova,

Jovanova,

Polizzi

et al. 2024

Medicina

View full text Add to dashboard Cite

show abstract

Extracellular Vesicles from Compression‐Loaded Cementoblasts Promote the Tissue Repair Function of Macrophages

Yang,

Liu,

Guo

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Treatment strategies for hard tissue defects aim to establish a mineralized microenvironment that facilitates tissue remodeling. As a mineralized tissue, cementum shares a similar structure with bone and exhibits an excellent capacity to resist resorption under compression. Macrophages are crucial for mineralized remodeling; however, their functional alterations in the microenvironment of cementum remain poorly understood. Therefore, this study explores the mechanisms by which cementum resists resorption under compression and the regulatory roles of cementoblasts in macrophage functions. As a result, extracellular vesicles from compression‐loaded cementoblasts (Comp‐EVs) promote macrophage M2 polarization and enhance the clearance of apoptotic cells (efferocytosis) by 2‐ to 3‐fold. Local injection of Comp‐EVs relieves cementum destruction in mouse root resorption model by activating the tissue repair function of macrophages. Moreover, Comp‐EV‐loaded hydrogels achieve significant bone healing in calvarial bone defect. Unexpectedly, under compression, EV secretion in cementoblasts is reduced by half. RNA‐Seq analysis and verification reveal that Rab35 expression decreases by 60% under compression, thereby hampering the release of EVs. Rab35 overexpression is proposed as a modification of cementoblasts to boost the yield of Comp‐EVs. Collectively, Comp‐EVs activate the repair function of macrophages, which will be a potential therapeutic strategy for hard tissue repair and regeneration.

show abstract

Understanding exosomes: Part 3—therapeutic + diagnostic potential in dentistry

Miron,

Estrin,

Sculean

et al. 2024

Periodontology 2000

View full text Add to dashboard Cite

Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with the ability to communicate with other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due to their ability to be utilized as therapeutic options for a wide array of various diseases. Over 5000 publications are currently being published on this topic yearly, many of which in the dental space. This extensive review article is the first scoping review aimed at summarizing all therapeutic uses of exosomes in regenerative dentistry. A total of 944 articles were identified as using exosomes in the dental field for either their regenerative/therapeutic potential or for diagnostic purposes derived from the oral cavity. In total, 113 research articles were selected for their regenerative potential (102 in vitro, 60 in vivo, 50 studies included both). Therapeutic exosomes were most commonly derived from dental pulps, periodontal ligament cells, gingival fibroblasts, stem cells from exfoliated deciduous teeth, and the apical papilla which have all been shown to facilitate the regenerative potential of a number of tissues including bone, cementum, the periodontal ligament, nerves, aid in orthodontic tooth movement, and relieve temporomandibular joint disorders, among others. Results demonstrate that the use of exosomes led to positive outcomes in 100% of studies. In the bone field, exosomes were found to perform equally as well or better than rhBMP2 while significantly reducing inflammation. Periodontitis animal models were treated with simple gingival injections of exosomes and benefits were even observed when the exosomes were administered intravenously. Exosomes are much more stable than growth factors and were shown to be far more resistant against degradation by periodontal pathogens found routinely in a periodontitis environment. Comparative studies in the field of periodontal regeneration found better outcomes for exosomes even when compared to their native parent stem cells. In total 47 diagnostic studies revealed a role for salivary/crevicular fluid exosomes for the diagnosis of birth defects, cardiovascular disease, diabetes, gingival recession detection, gingivitis, irritable bowel syndrome, neurodegenerative disease, oral lichen planus, oral squamous cell carcinoma, oropharyngeal cancer detection, orthodontic root resorption, pancreatic cancer, periodontitis, peri‐implantitis, Sjögren syndrome, and various systemic diseases. Hence, we characterize the exosomes as possessing “remarkable” potential, serving as a valuable tool for clinicians with significant advantages.

show abstract

Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity

Cited by 3 publications

References 78 publications

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Extracellular Vesicles from Compression‐Loaded Cementoblasts Promote the Tissue Repair Function of Macrophages

Understanding exosomes: Part 3—therapeutic + diagnostic potential in dentistry

Contact Info

Product

Resources

About