Effect of human embryonic stem cell-derived neuronal precursor cell transplantation into the cerebral infarct model of rat with exercise

Kim, Dae Yul; Park, Sung Sup; Lee, Si-Uk; Choi, Deok-Hyung; Park, Hee-Won; Paek, Sun Ha; Shin, Hye Young; Kim, Eun Young; Park, Se-Pill; Lim, Jin Ho

doi:10.1016/j.neures.2007.02.016

Cited by 34 publications

(25 citation statements)

References 49 publications

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“…Neural precursor cells that have been used in rodent models include conditionally immortalized cell lines derived from human fetal tissue (Pollock et al , 2006; Stevanato et al , 2009), carcinomas (Bliss et al , 2006; Hara et al , 2008), fetal neuronal stem cells (Zhang et al , 2009a), mouse neural precursors derived from the post-stroke cortex (Nakagomi et al , 2009), and precursors derived from mouse (Theus et al , 2008a; Wei et al , 2005; Yanagisawa et al , 2006) or human (Daadi et al , 2008b; Kim et al , 2007b) embryonic stem cells. Undifferentiated pluripotent stem cells are normally treated with a neural induction protocol before transplantation to gain cell lineage direction as well as reduce the risk of tumorigenesis (Newman et al , 2005).…”

Section: Stem Cells Are Pluripotent/multipotent Cells Capable Of Tissmentioning

confidence: 99%

“…Nonetheless, the neurogenic activity induced new cells usually do not survive well during the migration and after arrival at the injury site (Capowski et al , 2007; Daadi et al , 2008a; Kim et al , 2007a; Roy et al , 2006; Yang et al , 2008). The repair capability of newly formed neurons is very limited and does not suffice for an effective tissue repair after brain injury (Grégoire et al , 2015).…”

Section: Mechanisms Underlying Stem Cell Therapymentioning

confidence: 99%

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Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke

Wei

Jiang

et al. 2017

Progress in Neurobiology

127

117

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One of the exciting advances in modern medicine and life science is cell-based neurovascular regeneration of damaged brain tissues and repair of neuronal structures. The progress in stem cell biology and creation of adult induced pluripotent stem (iPS) cells has significantly improved basic and pre-clinical research in disease mechanisms and generated enthusiasm for potential applications in the treatment of central nervous system (CNS) diseases including stroke. Endogenous neural stem cells and cultured stem cells are capable of self-renewal and give rise to virtually all types of cells essential for the makeup of neuronal structures. Meanwhile, stem cells and neural progenitor cells are well-known for their potential for trophic support after transplantation into the ischemic brain. Thus, stem cell-based therapies provide an attractive future for protecting and repairing damaged brain tissues after injury and in various disease states. Moreover, basic research on naïve and differentiated stem cells including iPS cells has markedly improved our understanding of cellular and molecular mechanisms of neurological disorders, and provides a platform for the discovery of novel drug targets. The latest advances indicate that combinatorial approaches using cell based therapy with additional treatments such as protective reagents, preconditioning strategies and rehabilitation therapy can significantly improve therapeutic benefits. In this review, we will discuss the characteristics of cell therapy in different ischemic models and the application of stem cells and progenitor cells as regenerative medicine for the treatment of stroke.

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Section: Stem Cells Are Pluripotent/multipotent Cells Capable Of Tissmentioning

confidence: 99%

Section: Mechanisms Underlying Stem Cell Therapymentioning

confidence: 99%

Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke

Wei

Jiang

et al. 2017

Progress in Neurobiology

127

117

View full text Add to dashboard Cite

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“…The lesion size was estimated as a percentage of the whole brain according to an earlier report (Kim et al, 2007) using the following formula: [(area of contralateral hemisphere)À (area of remaining ipsilateral hemisphere)] Â (100/2)/(area of contralateral hemisphere). The areas of both hemispheres were measured on eight serial coronal sections per brain (2 mm apart) and stained with Cresyl Violet, and the area of the infarction was averaged over eight levels.…”

Section: Measurement Of Infarct Volumementioning

confidence: 99%

Neuroprotective Effects of Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells in Transient Focal Cerebral Ischemia in Rats

Liu

Seçkin

İzci

et al. 2009

J Cereb Blood Flow Metab

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Embryonic mesenchymal stem cells (eMSCs) were first derived from human embryonic stem cells (hESCs) overexpressing green fluorescence protein (GFP). They expressed CD29, CD44, CD73, CD105, CD166 and nestin, but not CD34, CD45, CD106 SSEA-4 or Oct3/4. Twenty million eMSCs in 1 mL of phosphate-buffered saline (PBS) were injected into the femoral veins of spontaneously hypertensive rats after transient middle cerebral artery occlusion. The migration and differentiation of the eMSCs in the ischemic brain were analyzed. The results revealed that eMSCs migrated to the infarction region and differentiated into neurons, which were positive for b-tubulin III, microtubule-associated protein 2 (MAP2), HuC, neurofilament and human nuclear antibody, and to vascular endothelial cells, which were positive for von Willebrand factor (vWF). The transplanted cells survived in the infarction region for at least 4 weeks. Adhesive removal function significantly improved in the first week after cell transplantation, and rotarod motor function significantly improved starting from the second week. The infarction volume in the eMSC group was significantly smaller than that in the PBS control group at 4 weeks after infusion. The results of this study show that when administered intravenously, eMSCs differentiated into neuronal and endothelial cells, reduced the infarction volume, and improved behavioral functional outcome significantly in transient focal cerebral ischemia.

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“…injection of ketamine (75 mg/kg) and xylazine (10 mg/ kg). We induced transient left middle cerebral artery occlusion (MCAo) for 90 min as previously described [10][11] . Briefly, a 4-0 nylon monofilament coated with silicone was inserted from the left common carotid artery (CCA) via the internal carotid artery to the base of the left MCA.…”

Section: Middle Cerebral Artery Occlusion Modelmentioning

confidence: 99%

Transplantation of mouse embryonic stem cell after middle cerebral artery occlusion

Lee

2012

Acta Cir. Bras.

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PURPOSE: Stem cell transplantation has been extensively studied as individual therapies for ischemic stroke. The present investigation is an initial effort to combine these methods to achieve increased therapeutic effects after brain ischemia. Cell transplantation may recover massive neuronal loss by replacing damaged brain cells. METHODS: Undifferentiated mouse embryonic stem (mES) cells were used to induce differentiation in vitro into neuron-like cells with good cell viability for use a graft. In this study, middle cerebral artery occlusion (MCAO) was induced in rats using intra-luminal vascular occlusion, and infused mES cells after MCAO. The animals were examined behaviorally using motor and sensory test with neurological assessment. RESULTS: Motor function of the recipients was gradually improved, whereas little improvement was observed in control rats. This result may suggest that the grafted cells have synaptic connection in the recipient brain. Our study revealed that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Consequently after euthanasia, rats were histochemically investigated to explore graft survival with green fluorescent protein (GFP). CONCLUSION: The mouse embryonic stem cells may have advantage for use as a donor source in various neurological disorders including motor dysfunction.

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Effect of human embryonic stem cell-derived neuronal precursor cell transplantation into the cerebral infarct model of rat with exercise

Cited by 34 publications

References 49 publications

Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke

Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke

Neuroprotective Effects of Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells in Transient Focal Cerebral Ischemia in Rats

Transplantation of mouse embryonic stem cell after middle cerebral artery occlusion

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