Effect of Host Resistance on Pathogenesis of Maize Dwarf Mosaic Virus

Law, Marc; Moyer, J. W.; Payne, Gary A.

doi:10.1094/phyto-79-757

Cited by 26 publications

(17 citation statements)

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“…Resistance to long distance transport within the plant has been suggested as a possible mechanism of virus resistance in crops (Ponz and Bruening, 1986). Our results show that the systemic movement and multiplication of BLRV was slower in resistant genotypes than it was in susceptible lentil and faba bean genotypes, which confirms previous results for some other viruses (Jensen, 1973; Doufour et al, 1989; Law et al, 1989; Nono‐Womdim et al, 1991; Makkouk and Ghulam, 1992; Makkouk and Kumari, 1993; Makkouk et al, 1994; Jbara, 2000).…”

Section: Discussionsupporting

confidence: 91%

Differentiation Among Bean Leafroll Virus Susceptible and Resistant Lentil and Faba Bean Genotypes on the Basis of Virus Movement and Multiplication

Kumari¹,

Makkouk²

2003

Journal of Phytopathology

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Systemic movement of Bean leafroll virus (BLRV) in susceptible and resistant lentil and faba bean genotypes was studied using plants grown in a plastic house. All the plants studied were inoculated with BLRV by viruliferous pea aphids (Acyrthosiphon pisum). Five plants/genotype of lentil and faba bean were harvested, respectively, at 3, 6, 9, 12 and 18 days and 1, 2, 3, 4 and 5 weeks after inoculation. Each plant was split into growing point, stem, stem base and root, and each was tested using tissue blot immunoassays (TBIA). Virus concentration in each section was estimated using a 0–3 score and a relative TBIA value was estimated accordingly for each genotype. In susceptible lentil genotypes (ILL 8063 and ILL 2581), BLRV was present in low concentrations in the growing point 3 days after inoculation and in high concentrations in all parts of the plant after 6 days. By contrast, the virus was not detected in the highly resistant genotype (ILL 74) until 18 days after inoculation. In the faba bean genotypes studied, BLRV was detected in high concentrations in all parts of the highly susceptible genotype (Fiord) 1 week after inoculation but only after 3 weeks in resistant genotypes (e.g. BPL 5274), but was not detected in the highly resistant genotypes (BPL 5278 and BPL 5279) 5 weeks after inoculation. The replication and systemic movement of BLRV was thus slower in resistant genotypes than in susceptible genotypes. Moreover, the use of TBIA scores clearly and easily differentiated resistant and susceptible genotypes. Our results suggest that BLRV movement and multiplication can be useful criteria when differentiating resistant from susceptible genotypes. In addition, undertaking the preliminary screening in a plastic house requires less space than direct planting in the field.

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Section: Discussionsupporting

confidence: 91%

Differentiation Among Bean Leafroll Virus Susceptible and Resistant Lentil and Faba Bean Genotypes on the Basis of Virus Movement and Multiplication

Kumari¹,

Makkouk²

2003

Journal of Phytopathology

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“…The relative restriction of virus movement in resistant cultivars has been reported for a number of sap‐transmissible viruses such as Cucumber mosaic virus (CMV) in pepper (Doufour et al., 1989; Nono‐Womdim et al., 1991) and Maize dwarf mosaic virus (MDMV) in maize (Law et al., 1989). Jensen (1973) had reported earlier that BYDV moves from an inoculated leaf more readily to roots in susceptible than in resistant cereals, but he did not measure the time required for the virus to move from the inoculated leaf to the root system.…”

Section: Introductionmentioning

confidence: 99%

Screening for Barley yellow dwarf virus‐Resistant Barley Genotypes by Assessment of Virus Content in Inoculated Seedlings

Šíp

Širlová

Chrpová

2006

Journal of Phytopathology

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The content of Barley yellow dwarf virus (BYDV) in roots and leaves of barley seedling plants differing in their level of resistance was assessed by quantitative ELISA 1-42 days after inoculation with the strain of BYDV (PAV). High virus accumulation in roots and low concentration in leaves was characteristic of the period 9-15 days after inoculation. In leaves, the differences in virus content between resistant and susceptible genotypes became significant after 15 days and resistance to virus accumulation was better expressed 30-39 days after inoculation. Roots of resistant materials exhibited evident retardation of virus accumulation and the greatest difference in virus content between resistant and susceptible plants was detected 9 days after inoculation. By these criteria, the selected winter and spring barley cultivars and lines (in total 44 materials) fell in to five groups according to field reactions and the presence or absence of the Yd2 resistance gene. There were highly significant and positive relations between ELISA values and 5-year field data on symptomatic reactions and grain-yield reductions due to infection. Using the described method, resistant and moderately resistant genotypes (both Yd2 and non-Yd2) were significantly differentiated from susceptible genotypes. The possible use of this method in screening for BYDV resistance is discussed.www.blackwell-synergy.com

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“…MDMV levels in inoculated leaves of the resistant line Pa405 were similar to those in susceptible controls, and virus spread within inoculated leaves could be detected serologically (Law, Moyer, & Payne, 1989;Lei & Agrios, 1986). Similarly, when germinating kernels of Pa405 were inoculated with MDMV by VPI, bright mosaic symptoms developed and MDMV antigen could be detected in leaves that were present in the embryo at the time of inoculation, but no systemic infection occurred (Cassone et al, 2014;Louie & Abt, 2004).…”

Section: Mechanisms Of Virus Resistance In Maizementioning

confidence: 80%