Effect of Host Medium on the Fluorescence Emission Intensity of Rhodamine B in Liquid and Solid Phase

Fikry, Mohamed; Omar, M. M.; Ismail, L.Z.

doi:10.1142/9789814317511_0025

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…Remarkably, fluorescence emission peaks are clearly visible after 60 h of exposure, and emissions tend to be higher when compared with spectra relative to the sample not exposed to UV light. This phenomenon can be likely attributed to quenching effects because of UV radiation that are widely documented for rhodamine dyes (Kubista et al ., ; Kurian et al ., ; Fikry et al ., ). Specifically, peak emissions tend to increase from exposure times of 6–24 h. Thereafter, emissions consistently decrease.…”

Section: Resultsmentioning

confidence: 97%

Diatom percolation through soils: a proof of concept laboratory experiment

et al. 2015

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Diatoms are unicellular eukaryotic algae that are widely distributed in aquatic environments. In particular, their presence is abundant in stream flows, whereas the instance and transport of cells through subsurface flow is controversial. In this work, we study the transport of diatoms through soils by designing laboratory percolation experiments on undisturbed loamy and sandy soil cores. To label diatom tracers and enhance their detectability, we develop protocols to functionalize frustules of purified diatomite and Conticribra weissflogii cultures with rhodamine 123 and 2‐(4‐pyridyl)‐5[4‐dimethylaminoethyl‐aminocarbamoyl)‐methoxy]phenyloxazole, respectively. Labelled diatoms demonstrate good detectability and resilience under weathering agents. We execute percolation experiments for maximum time domains of approximately 600 min where volumes of water up to 3600 ml are deployed on the soil core surface. Under such experimental conditions, fluorescent diatoms are not recovered in samples of percolated material as per microscopy and spectrofluorometry. While further field studies are needed to fully investigate diatom transport, our findings shed new light on the potential of using biotic tracers for surface hydrological processes. Copyright © 2015 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 97%

Diatom percolation through soils: a proof of concept laboratory experiment

et al. 2015

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“…The red-shift in fluorescence with increased concentration from the TAMRA-labeled peptide likely results from a quenching effect, which occurs when more of the shorter wavelengths of fluorescence becomes absorbed with a reduced average distance between dye molecules. 36 We do not expect this shift to introduce crosstalk in the multispectral images because the peptides bind at low concentration. In addition, CF633 belongs to the carbocyanine family along with indocyanine green, another FDA-approved dye.…”

Section: Discussionmentioning

confidence: 98%

Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy

Joshi¹,

Miller²,

Lee³

et al. 2012

SPIE Proceedings

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Abstract. Gastrointestinal cancers are heterogeneous and can overexpress several protein targets that can be imaged simultaneously on endoscopy using multiple molecular probes. We aim to demonstrate a multispectral scanning fiber endoscope for wide-field fluorescence detection of colonic dysplasia. Excitation at 440, 532, and 635 nm is delivered into a single spiral scanning fiber, and fluorescence is collected by a ring of light-collecting optical fibers placed around the instrument periphery. Specific-binding peptides are selected with phage display technology using the CPC;Apc mouse model of spontaneous colonic dysplasia. Validation of peptide specificity is performed on flow cytometry and in vivo endoscopy. The peptides KCCFPAQ, AKPGYLS, and LTTHYKL are selected and labeled with 7-diethylaminocoumarin-3-carboxylic acid (DEAC), 5-carboxytetramethylrhodamine (TAMRA), and CF633, respectively. Separate droplets of KCCFPAQ-DEAC, AKPGYLS-TAMRA, and LTTHYKL-CF633 are distinguished at concentrations of 100 and 1 μM. Separate application of the fluorescent-labeled peptides demonstrate specific binding to colonic adenomas. The average target/background ratios are 1.71 AE 0.19 and 1.67 AE 0.12 for KCCFPAQ-DEAC and AKPGYLS-TAMRA, respectively. Administration of these two peptides together results in distinct binding patterns in the blue and green channels. Specific binding of two or more peptides can be distinguished in vivo using a novel multispectral endoscope to localize colonic dysplasia on real-time wide-field imaging.

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“…Quenching refers to a variety of processes that can decrease the fluorescence intensity of a labeled sample (Penzkofer & Lu, 1986;Valeur, 2001). In ethanol, for example, RB at high concentrations can form monomer dimmers and higher aggregates that restrict the fluorescence emission intensity (Fikry et al, 2011). In the nonsimplified adhesives, the higher viscosity of the media reduces the molecular diffusion, constraining the movement of dye molecules necessary to allow specific molecular interactions and nonradiative energy loss that lead to quenching.…”

Section: Photoluminescence Spectroscopy Of Rb-labeled Adhesivesmentioning

confidence: 99%

Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface

et al. 2017

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Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to determine suitable concentrations of RB in these bonding agents for imaging in the confocal laser scanning microscope. This study provides systematical strategies for adding RB to viscous dental adhesive resins, focusing on the determination of the lowest range of dye concentrations necessary to achieve an acceptable image of the dentin/adhesive interface. It was demonstrated that optimized images of the resin distribution in dentin can be produced with 0.1-0.02 mg/mL of RB in the (tested) adhesives. Our approaches took into account aspects related to the dye concentration, photophysical parameters in different host media, specimen composition and morphology to develop a rational use of the fluorescent agent with the resin-based materials. Information gained from this work can help optimize labeling methods using dispersions of low-molecular-weight dyes in different monomer blend systems.

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Effect of Host Medium on the Fluorescence Emission Intensity of Rhodamine B in Liquid and Solid Phase

Cited by 9 publications

References 20 publications

Diatom percolation through soils: a proof of concept laboratory experiment

Diatom percolation through soils: a proof of concept laboratory experiment

Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy

Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface

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