Effect of holding buffy coats 4 or 18 hours before preparing pooled filtered PLT concentrates in plasma

Abstract

In PCs prepared after holding BCs 4 or 18 hours before pooling and filtering, only a minor significant difference in the percentage of PLTs with bound fibrinogen was found after 5 days of storage. This difference is probably of little, if any, transfusional significance.

Stored platelets contain residual amounts of tissue factor: evidence from studies on platelet concentrates stored for prolonged periods

“…The deterioration of adhesive functions was progressive with storage time and associated with an increase in the surface expression of activation‐dependent antigens as assessed by flow cytometry. Increased percentages of PLTs exposing P‐selectin and LIMP have been considered as indicators of the PLT storage lesion 31,32 . In our study, the loss of adhesive properties seemed to parallel a progressive enhancement in anionic phospholipids exposures on stored PLTs as indicated by increased binding of annexin V in flow cytometry and immunocytochemical studies.…”

“…In our study, the loss of adhesive properties seemed to parallel a progressive enhancement in anionic phospholipids exposures on stored PLTs as indicated by increased binding of annexin V in flow cytometry and immunocytochemical studies. Several authors have already described the loss of membrane asymmetry and increase of exposure of anionic phospholipids in PLTs 32,33 . Loss of membrane integrity in cells and exposure of phosphatidylserine is linked to apoptosis, but also with the development of PCA 9 .…”

The Effect of Holding Times of Whole Blood and Its Components During Processing on In Vitro and In Vivo Quality

“…A study comparing 4-hour-held buffy coat prepared from overnightheld whole blood vs a less than 4-hour hold of whole blood followed by 18-hour hold of the buffy coats prior to buffy coat pooling showed no differences in cell counts and minor differences in storage characteristics of subsequently prepared platelet concentrates [42]. They saw a slight but significant increase in platelet activation markers (such as CD62P expression, lysosomal integral membrane protein, annexin A5, factor V-Va, bound von Willebrand factor [vWF], and bound fibrinogen) during the holding period, but similar to the findings of Van der Meer et al [31], this dropped to baseline levels once the platelet concentrates were prepared.…”

Wissenschaftliche Erläuterungen zur Stellungnahme „Bewertung von Apherese- und Pool-Thrombozytenkonzentraten“ des AK Blut vom 31.03.2015

“…Die aktuelle Datenlage bietet keinen Anhalt dafür, dass eine Raumtemperaturlagerung der Vollblutspenden bis zur PTK-Herstellung einen nachteiligen Einfluss auf die PTK-Qualität hat, solange das Intervall geringer als 24 h ist [14][15][16][17][18][19][20][21] [57,[59][60][61][62].…”