Effect of HIV infection in the micronuclei frequency on the oral mucosa

Lima, Carlos H. M. de; Alves, Mônica Ghislaine Oliveira; Furtado, Juvencio; Marcucci, Marcelo; Balducci, Ivan; Almeida, Janete Días

doi:10.1111/jop.12527

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…Our findings are in line with those of Lima and colleagues that show an increase in the frequency of multiple MN in oral mucosa cells in HIV patients compared to healthy controls [35]. …”

Section: Discussionsupporting

confidence: 93%

HIV infection and frequency of micronucleus in human peripheral blood cells

Zizza

Grima

Andreassi

et al. 2019

Journal of Preventive Medicine and Hygiene

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“…Our findings are in line with those of Lima and colleagues that show an increase in the frequency of multiple MN in oral mucosa cells in HIV patients compared to healthy controls [35]. …”

Section: Discussionsupporting

confidence: 93%

HIV infection and frequency of micronucleus in human peripheral blood cells

Zizza

Grima

Andreassi

et al. 2019

Journal of Preventive Medicine and Hygiene

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“…In 2013, Olivero et al 24 observed that the use of ART, with reverse transcriptase inhibitor drugs (zidovudine, lamivudine, abacavir, and nevirapine) in the offspring of pregnant monkeys not carrying HIV, which were medicated during pregnancy and after birth, revealed genomic instability and mutagenic effects that persisted for 3 years (final year of research). Moraes Filho et al 25 evaluated that cytotoxicity and genotoxicity of different concentrations of ART composed of tenofovir, lamivudine, and zidovudine, lamivudine, and efavirenz, in mice, through the comet assay and bone marrow micronucleus test. The increase of MN in buccal mucosal cells was observed by Lima et al 26 , in adults with HIV using ART, with low viral load.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic changes in oral mucosal cells of human immunodeficiency virus-infected children and adolescents undergoing antiretroviral treatment

Alpire,

Souza,

Masutti

et al. 2023

Rev. Assoc. Med. Bras.

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SUMMARY OBJECTIVE: The objective of this study was to evaluate possible cytogenetic changes in children and adolescents with human immunodeficiency virus on antiretroviral therapy, through the micronucleus test in oral mucosa. METHODS: This was a prospective study consisted of 40 individuals, of whom 21 comprised the human immunodeficiency virus group and 19 comprised the control group. Children and adolescents with human immunodeficiency virus were enrolled. The inclusion criteria were <18 years old and consent in participating in the study. The exclusion criteria were the presence of numerous systemic comorbidities, oral lesions, the habit of smoking, alcohol consumption, and X-rays or CT scans taken within 15 days prior to sample collection. A gentle scraping was performed on the inner portion of the jugal mucosa on both sides. A total of 2,000 cells per slide were analyzed for the determination of mutagenicity parameters as follows: micronuclei, binucleation, and nuclear buds. For measuring cytotoxicity, the following metanuclear changes were evaluated: pyknosis, karyolysis, and karyorrhexis, in a double-blind manner. The repair index was also evaluated in this setting. RESULTS: The human immunodeficiency virus group showed high frequencies of micronuclei (p=0.05), binucleated cells (p=0.001), and nuclear buds (p=0.03). In the cytotoxicity parameters, represented by the cell death phases, there was an increase with statistical difference (p≤0.05) in the karyorrhexis frequency (p=0.05). Additionally, repair index was decreased in the human immunodeficiency virus group. CONCLUSION: These results indicate that human immunodeficiency virus -infected individuals undergoing antiretroviral therapy have cytogenetic changes in oral mucosal cells.

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“…In Brazil, Lima et al [ 32 ] investigated micronuclei in exfoliated oral cells of HIV-infected individuals undergoing ART and non-infected controls ( n = 30 each group). In addition to measuring MN frequency, the authors separated micronuclei into two categories: (I) single MN and (II) multiple micronuclei.…”

Section: Impacts Of Hiv Infection and Treatment On Chromosomal Integr...mentioning

confidence: 99%

“…Considering the two MN categories mentioned, a statistically significant increased mean of single MN in the cells of the controls compared to those of HIV-infected individuals and a non-significant increase in the occurrence of multiple micronuclei in the cells of the HIV group compared to the controls were reported. However, the small sample size of this study and the absence of difference in overall MN frequency between the groups may limit interpretations of these findings [ 32 ].…”

Section: Impacts Of Hiv Infection and Treatment On Chromosomal Integr...mentioning

confidence: 99%

HIV Infection, Chromosome Instability, and Micronucleus Formation

Ellwanger

Kulmann-Leal

Ziliotto

2023

Viruses

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Genome integrity is critical for proper cell functioning, and chromosome instability can lead to age-related diseases, including cancer and neurodegenerative disorders. Chromosome instability is caused by multiple factors, including replication stress, chromosome missegregation, exposure to pollutants, and viral infections. Although many studies have investigated the effects of environmental or lifestyle genotoxins on chromosomal integrity, information on the effects of viral infections on micronucleus formation and other chromosomal aberrations is still limited. Currently, HIV infection is considered a chronic disease treatable by antiretroviral therapy (ART). However, HIV-infected individuals still face important health problems, such as chronic inflammation and age-related diseases. In this context, this article reviews studies that have evaluated genomic instability using micronucleus assays in the context of HIV infection. In brief, HIV can induce chromosome instability directly through the interaction of HIV proteins with host DNA and indirectly through chronic inflammation or as a result of ART use. Connections between HIV infection, immunosenescence and age-related disease are discussed in this article. The monitoring of HIV-infected individuals should consider the increased risk of chromosome instability, and lifestyle interventions, such as reduced exposure to genotoxins and an antioxidant-rich diet, should be considered. Therapies to reduce chronic inflammation in HIV infection are needed.

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Effect of HIV infection in the micronuclei frequency on the oral mucosa

Cited by 8 publications

References 26 publications

HIV infection and frequency of micronucleus in human peripheral blood cells

HIV infection and frequency of micronucleus in human peripheral blood cells

Cytogenetic changes in oral mucosal cells of human immunodeficiency virus-infected children and adolescents undergoing antiretroviral treatment

HIV Infection, Chromosome Instability, and Micronucleus Formation

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