Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3

Kim, Jiyoung; Kim, Yeonsoo

doi:10.1038/s41598-018-27008-w

Cited by 12 publications

(7 citation statements)

References 46 publications

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“…1A). Numerous research groups have demonstrated that these RPs may be utilized by diverse viruses for efficient viral infection (25,30,(37)(38)(39)(40)(41)(42)(43)(44). Notably, the candidate protein RPL13, a member of the L13E family, is conserved in a wide range of species, including plants, yeast, and animals (45,46).…”

Section: Resultsmentioning

confidence: 99%

Ribosomal Protein L13 Promotes IRES-Driven Translation of Foot-and-Mouth Disease Virus in a Helicase DDX3-Dependent Manner

Han

Sun

et al. 2020

J Virol

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Internal ribosome entry site (IRES)-driven translation is a common strategy among positive-sense, single-stranded RNA viruses for bypassing the host cell requirement of a 5′ cap structure. In the current study, we identified the ribosomal protein L13 (RPL13) as a critical regulator of IRES-driven translation of foot-and-mouth disease virus (FMDV) but found that it is not essential for cellular global translation. RPL13 is also a determinant for translation and infection of Seneca Valley virus (SVV) and classical swine fever virus (CSFV), and this suggests that its function may also be conserved in unrelated IRES-containing viruses. We further showed that depletion of DEAD box helicase DDX3 disrupts binding of RPL13 to the FMDV IRES, whereas the reduction in RPL13 expression impairs the ability of DDX3 to promote IRES-driven translation directly. DDX3 cooperates with RPL13 to support the assembly of 80S ribosomes for optimal translation initiation of viral mRNA. Finally, we demonstrated that DDX3 affects the recruitment of the eukaryotic initiation factor eIF3 subunits e and j to the viral IRES. This work provides the first connection between DDX3 and eIF3e/j and recognition of the role of RPL13 in modulating viral IRES-dependent translation. This previously uncharacterized process may be involved in selective mRNA translation. IMPORTANCE Accumulating evidence has unveiled the roles of ribosomal proteins (RPs) belonging to the large 60S subunit in regulating selective translation of specific mRNAs. The translation speciﬁcity of the large-subunit RPs in this process is thought provoking, given the role they play canonically in catalyzing peptide bond formation. Here, we have identified the ribosomal protein L13 (RPL13) as a critical regulator of IRES-driven translation during FMDV infection. Our study supports a model whereby the FMDV IRESs recruit helicase DDX3 recognizing RPL13 to facilitate IRES-driven translation, with the assistance of eIF3e and eIF3j. A better understanding of these specific interactions surrounding IRES-mediated translation initiation could have important implications for the selective translation of viral mRNA and thus for the development of effective prevention of viral infection.

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Section: Resultsmentioning

confidence: 99%

Ribosomal Protein L13 Promotes IRES-Driven Translation of Foot-and-Mouth Disease Virus in a Helicase DDX3-Dependent Manner

Han

Sun

et al. 2020

J Virol

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“…It has been shown that RPS3 plays an important role in cell mitosis by interacting with α-tubulin. When cells are infected with HIV-1, HIV-1 Tat protein can interact with RPS3 and disturb its localization on the spindle, and ultimately inhibit cell proliferation [18]. Moreover, the function of RPS3 depends on its own nuclear translocation, which is regulated by protein kinases such as SRC [19].…”

Section: Regulation Network After Infectionmentioning

confidence: 99%

Assessing ACE2 expression patterns in lung tissues in the pathogenesis of COVID-19

Zhang

et al. 2020

Journal of Autoimmunity

254

243

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It has been reported that SARS-CoV-2 may use ACE2 as a receptor to gain entry into human cells, in a way similar to that of SARS-CoV. Analyzing the distribution and expression level of ACE2 may therefore help reveal underlying mechanisms of viral susceptibility and post-infection modulation. In this study, we utilized previously uploaded information on ACE2 expression in various conditions including SARS-CoA to evaluate the role of ACE2 in SARS-CoV and extrapolate that to COVID-19. We found that the expression of ACE2 in healthy populations and patients with underlying diseases was not significantly different. However, based on the elevated expression of ACE2 in cigarette smokers, we speculate that long-term smoking may be a risk factor for COVID-19. Analysis of ACE2 in SARS-CoV infected cells suggests that ACE2 is not only a receptor but is also involved in post-infection regulation, including immune response, cytokine secretion, and viral genome replication. Moreover, we constructed Protein-protein interaction (PPI) networks and identified hub genes in viral activity and cytokine secretion. Our findings may help clinicians and researchers gain more insight into the pathogenesis of SARS-CoV-2 and design therapeutic strategies for COVID-19.

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“…As regards alphaviruses, RPSA includes different binding sites for Venezualian equine encephalitis virus (VEEV) and Sindbis virus (SINV) (Jamieson et al 2008 ; Malygin et al 2009 ). HIV-1 Tat inhibits cell proliferation via an interaction with RPS3 and increases the level of RPS3 in the nucleus, thereby disrupting mitotic spindle formation during HIV-1 infection (Kim and Kim 2018 ). FMDV VP1 inhibits the antiviral response of RPSA by interacting with RPSA to promote FMDV replication (Zhu et al 2019 ).…”

Section: Alterations In Functional Characteristics Of Rps For Viral Tmentioning

confidence: 99%

Selective regulation in ribosome biogenesis and protein production for efficient viral translation

Dong

Zhang

et al. 2020

Arch Microbiol

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As intracellular parasites, viruses depend heavily on host cell structures and their functions to complete their life cycle and produce new viral particles. Viruses utilize or modulate cellular translational machinery to achieve efficient replication; the role of ribosome biogenesis and protein synthesis in viral replication particularly highlights the importance of the ribosome quantity and/or quality in controlling viral protein synthesis. Recently reported studies have demonstrated that ribosome biogenesis factors (RBFs) and ribosomal proteins (RPs) act as multifaceted regulators in selective translation of viral transcripts. Here we summarize the recent literature on RBFs and RPs and their association with subcellular redistribution, post-translational modification, enzyme catalysis, and direct interaction with viral proteins. The advances described in this literature establish a rationale for targeting ribosome production and function in the design of the next generation of antiviral agents.

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Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3

Cited by 12 publications

References 46 publications

Ribosomal Protein L13 Promotes IRES-Driven Translation of Foot-and-Mouth Disease Virus in a Helicase DDX3-Dependent Manner

Ribosomal Protein L13 Promotes IRES-Driven Translation of Foot-and-Mouth Disease Virus in a Helicase DDX3-Dependent Manner

Assessing ACE2 expression patterns in lung tissues in the pathogenesis of COVID-19

Selective regulation in ribosome biogenesis and protein production for efficient viral translation

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