Effect of histamine on lipid composition of rat bile

Abstract

To investigate the effect of histamine (8 mkg/kg, i/v) on the concentration of lipids in the rats bile. Using the method of thin layer chromatography concentrations of phospholipids, cholesterol and its esters, free fatty acids, triglycerides were determined in the rats bile collected in acute experiments (anesthesia, sodium thiopental, 50 mg/kg). The results were calculated using Statistica 7.0; p<0,05. Histamine increased concentration of phospholipids, cholesterol, its esters and reduced content of free fatty acids and triglycerides in the rats bile. Probably, histamine, acting on transport lipid components in bile effect on its physical and chemical properties and the maintenance of cholesterol in mixed bile micelles. Histamine affects the metabolic conversion and transport of lipids in the liver cells, causing the increase in the concentration of phospholipids, cholesterol and its esters in bile and decrease it in the content of free fatty acids and triglycerides.