Effect of high temperature on dehydration-induced alterations in photosynthetic characteristics of the resurrection plant Haberlea rhodopensis

Abstract

The effect of high temperature (HT) and dehydration on the activity of photosynthetic apparatus and its ability to restore membrane properties, oxygen evolution, and energy distribution upon rehydration were investigated in a resurrection plant, Haberlea rhodopensis. Plants growing under low irradiance in their natural habitat were desiccated to air-dry state at a similar light intensity [about 30 μmol(photon) m -2 s -1 ] under optimal day/night (23/20C) or high (38/30C) temperature. Our results showed that HT alone reduced the photosynthetic activity and desiccation of plants at 38C and it had more detrimental effect compared with desiccation at 23°C. The study on isolated thylakoids demonstrated increased distribution of excitation energy to PSI as a result of the HT treatment, which was enhanced upon the desiccation. It could be related to partial destacking of thylakoid membranes, which was confirmed by electron microscopy data. In addition, the surface charge density of thylakoid membranes isolated from plants desiccated at 38°C was higher in comparison with those at 23°C, which was in agreement with the decreased membrane stacking. Dehydration led to a decrease of amplitudes of oxygen yields and to a loss of the oscillation pattern. Following rehydration, the recovery of CO 2 assimilation and fluorescence properties were better when desiccation was performed at optimal temperature compared to high temperature. Rehydration resulted in partial recovery of the amplitudes of flash oxygen yields as well as of population of S 0 state in plants desiccated at 23°C. However, it was not observed in plants dehydrated at 38°C.

Born to revive: molecular and physiological mechanisms of double tolerance in a paleotropical and resurrection plant

“…Changes in carbon assimilation of desiccation‐tolerant plants within the Gesneriaceae had previously been studied under desiccation/rehydration cycles (Schwab et al , 1989; Markovska et al , 1994; Georgieva et al , 2007; Degl’Innocenti et al , 2008) or under different controlled conditions of light, CO 2 concentration and temperature (Norwood et al , 2000; Peeva & Cornic, 2009; Velitchkova et al , 2013; Rakić et al , 2015). Our study contributes novel photosynthetic data directly collected under natural field conditions for the resurrection plant R. myconi .…”

Leaf metabolite profile of the Brazilian resurrection plant Barbacenia purpurea Hook. (Velloziaceae) shows two time-dependent responses during desiccation and recovering

“…This time of recovery seems to be necessary for regulation of metabolism and may be associated with the repair of possible damage at this stage, as suggested by other studies on resurrection plants (Oliver et al, 1998; Farrant et al, 2007; Lüttge et al, 2008; Farrant et al, 2012). Recently, Velitchkova et al (2013) demonstrated that high temperature has stronger physiological effects on the dehydrated plants of H. rhodopensis , a resurrection species of the northern hemisphere, reducing its capacity of recovering after rehydration, when compared to plants submitted to desiccation at optimal temperature.…”

Desiccation‐induced alterations in surface topography of thylakoids from resurrection plant Haberlea rhodopensis studied by atomic force microscopy, electrokinetic and optical measurements

“…For electrophoretic mobility (EPM) measurements, the thylakoids resuspended in 50 mM MES buffer (pH 6.0), 15 mM NaCl and 5 mM MgCl 2 were used. EPM was performed using the particle electrophoresis technique OPTON Cytophotometer (FeintechnikGes, m.b.H., Wien, Austria) as previously described (Velitchkova et al ). The zeta potential (electrokinetic potential, ζ) was calculated from the EPM, u, using Helmholtz–Smoluchowski equation (Hiemenz ).…”

“…The light scattering (LS) intensity measures the degree of volume changes of the thylakoids. The scattering level at an angle of 90° represented the degree of aggregation (Latimer , Doltchinkova et al , Velitchkova et al , Mihailova et al ).…”

“…The surface electric charge of thylakoid membranes was measured as a physicochemical parameter characterizing their stability (Doltchinkova et al , , Delgado et al ). Zeta potential had a significant role in controlling the interaction of biological cells with external influences such as drought (Velitchkova et al , Mihailova et al ). It has been shown that electrical properties of thylakoid membranes control thylakoid stacking into chloroplast (Barber , Chow , Puthiyaveetil et al ), membrane conformational changes, electron transport (Doltchinkova and Lambreva , Doltchinkova et al , Kramer et al , Chow et al ) and thylakoid membrane dynamics (Tikkanen et al , Schneider and Geissler ).…”