Effect of High Salt Treatment on Influenza B Viral Protein Synthesis in MDCK Cells

Abstract

Based on the information that high salt inhibits the initiation of cellular mRNA translation which depends on the function of the 5'-terminal structure of mRNA, we compared the effect of high salt on translation of host cellular mRNAs and influenza viral mRNAs, both of which are of 5 '-terminal structure. Brief exposure of influenza B virus-infected MDCK cells to high salt medium resulted in a dosedependent inhibition of viral polypeptide synthesis as well as of cellular polypeptide synthesis, but it had less effect on synthesis of viral polypeptides, particularly nonstructural protein (NS). Under these conditions the Nar content of the infected cells was significantly increased. A similar salt effect on in vitro translation of viral and cellular mRNAs extracted from infected cells was also observed. There was no significant difference in sensitivity to hypertonic block of in vivo translation of influenza viral mRNAs and vesicular stomatitis virus mRNAs, the latter of which possess a virus-directed structure at the 5 '-terminus.

Cation Radius Effects on Cell-Free Translation in Rabbit Reticulocyte Lysate

“…Several advantages of adding potassium chloride to rabbit reticulocyte reactions have been identified; e.g., KCl may improve the fidelity of initiation from capped (13) and uncapped (11) messages. On the other hand, sodium (14,15) and thallium (2) were reported to inhibit in vitro translation, although the latter is also known to stabilize K-depleted ribosomes at low concentrations. However, no systematic survey of cations has been accomplished yet.…”

Characterization of Bovine Viral Diarrhoea-Mucosal Disease Virus-specific Proteins in Bovine Cells

“…A hypertonic extracellular environment established by the addition of NaC1 shortly before the radiolabelling step was found to be an effective means of detecting virus-induced polypeptides since initiation of translation of cellular messages is more sensitive to extracellular hypertonicity than that of viral messages (Nuss et al, 1975). This procedure, known as HIB has been used in other virus systems to facilitate the identification of virus-induced polypeptides (Nuss et al, 1975;Tsurumi et al, 1983;Lamb et al, 1978). When 150 mi-NaC1 in excess of the normal NaC1 content of MEME was added to FBT cells, incorporation of radiolabelled amino acids in treated cultures was reduced to only 2 to 3 ~ of control values.…”

Development of saline loaded mask materials, evaluation of the antimicrobial efficacy and survivability of selected bacteria on these mask materials

“…In cells infected with reticuloendotheliosis virus (REV), saline solution was shown to interfere with viral protein synthesis and further release of virus from infected cells. Saline solution inhibited the protein synthesis of Influenza B virus infecting Madin-Darby Canine Kidney (MDCK) cells ( Bishop et al, 1976 , Tsumrumi et al, 1983 ). In addition to this, it has also been found to enhance viral adsorption to micro porous filters ( Lukasik et al, 2000 ).…”