Effect of High-Pressure Gas on Yeast Growth

Abstract

Microcalorimetry is a useful tool for monitoring the growth behavior of microorganisms. In this study, microcalorimetry was used to investigate the effects of nitrogen, air, oxygen, nitrous oxide, argon, and krypton at high pressure on the growth of the yeast Saccharomyces cerevisiae. Growth thermograms (metabolic heat vs. incubation time) were generated to estimate metabolic activity under compressed gases and to determine the 50% inhibitory pressure (IP(50)) and minimum inhibitory pressure (MIP), which are regarded as indices of the toxicity of compressed gases. Based on MIP values, the most toxic to the least toxic gases were found to be: O(2) > N(2)O > air > Kr > N(2) > Ar.

Exploration of the Effects of High Hydrostatic Pressure on Microbial Growth, Physiology and Survival: Perspectives from Piezophysiology

“…While nitrogen and argon retard a growth in range of 20-35 MPa, oxygen, nitrous oxide and air inhibit growth at pressures of less than 1 MPa probably due to oxidative stress. 60) Exposure of yeast cells to temperatures higher than the optimum for growth (e.g., 42 C) enhances the synthesis of Hsps and the metabolism of trehalose. 61) Then the cells acquire the ability to survive under lethally high temperatures (e.g., 50 C) as well as under other stressful conditions.…”

Effects of compression with ethane, ethylene and their fluorinated derivatives on yeast growth

“…The specific growth activities at various pressures were fitted after they were plotted against gas pressure. See other literatures for more details on the quantification of growth inhibition [3]. We determined both the 50% inhibitory pressure (IP 50 ), which reduces the growth of yeast by 50% and the minimum inhibitory pressure (MIP) at which yeast growth is completely inhibited.…”

“…Recently, we have proposed a new method to quantify the toxicity of various gases through observing the inhibition of yeast growth under high-pressure gases [3].Generally, at normal pressure, the solubilities of gases such as oxygen, nitrogen, hydrocarbon gases are low in aqueous systems. It is therefore difficult to evaluate the effects of gases on yeast growth in aqueous medium.…”

High‐pressure inactivation of dried microorganisms

“…As cellular concentration of inert gas increases under pressure, physiological perturbations occur, leading to growth inhibition of various organisms (Arao et al, 2005). Mechanical damage in cells pressurized in the presence of inert gas and rapidly decompressed have also been described (Gottlieb and Adachi, 2000;Yandell and McCarthy, 1980).…”