Effect of high-intensity light and UV-B on photosynthetic activity and the expression of certain light-responsive genes in A. thaliana phyA and phyB mutants

Kreslavski, Vladimir D.; Strokina, Valeria V.; Khudyakova, Alexandra; Ширшикова, Г. Н.; Kosobryukhov, A. A.; Pashkovskiy, Pavel P.; Alwasel, Saleh; Allakhverdiev, Suleyman I.

doi:10.1016/j.bbabio.2021.148445

Cited by 20 publications

(18 citation statements)

References 37 publications

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“…The quality of light influences photomorphogenesis, photosynthesis, and plant growth through appropriate photoreceptors [ 33 , 34 ]. However, no significant difference in the expression level of photoreceptor genes was found between the light variants BL and RL, which is consistent with previous studies performed on A. thaliana seedlings [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Light of Different Spectral Compositions on the Growth, Photosynthesis, and Expression of Light-Dependent Genes of Scots Pine Seedlings

Pashkovskiy

Kreslavski

Ivanov

et al. 2021

Cells

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Varying the spectral composition of light is one of the ways to accelerate the growth of conifers under artificial conditions for the development of technologies and to obtain sustainable seedlings required to preserve the existing areas of forests. We studied the influence of light of different quality on the growth, gas exchange, fluorescence indices of Chl a, and expression of key light-dependent genes of Pinus sylvestris L. seedlings. It was shown that in plants growing under red light (RL), the biomass of needles and root system increased by more than two and three times, respectively, compared with those of the white fluorescent light (WFL) control. At the same time, the rates of photosynthesis and respiration in RL and blue light (BL) plants were lower than those of blue red light (BRL) plants, and the difference between the rates of photosynthesis and respiration, which characterizes the carbon balance, was maximum under RL. RL influenced the number of xylem cells, activated the expression of genes involved in the transduction of cytokinin (Histidine-containing phosphotransfer 1, HPT1, Type-A Response Regulators, RR-A) and auxin (Auxin-induced protein 1, Aux/IAA) signals, and reduced the expression of the gene encoding the transcription factor phytochrome-interacting factor 3 (PIF3). It was suggested that RL-induced activation of key genes of cytokinin and auxin signaling might indicate a phytochrome-dependent change in cytokinins and auxins activity.

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Section: Discussionmentioning

confidence: 99%

Influence of Light of Different Spectral Compositions on the Growth, Photosynthesis, and Expression of Light-Dependent Genes of Scots Pine Seedlings

Pashkovskiy

Kreslavski

Ivanov

et al. 2021

Cells

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“…The dynamic energy pipeline leaf model expressing the energy flux parameters for electron transport per unit of sample area, trapping of excitation energy, and light absorption from Chl a fluorescence was derived in the case of rice imparted with 4–6 kJ m −2 d −1 UV-B; it was seen that parameters such as density of active reaction centres (RC/CS) were enhanced, which in turn increased the activity of the reaction centres. UV-treated plants also exhibited a reduced dissipation of energy, which led to an increase in photon absorption, electron transport, trapping of photons, and electron transport flux per reaction centre (ET O /CS M ) [ 70 ]. However, like other stresses, high-dosage UV-B (28 kJ m −2 d −1 ) can cause a reduction in and degradation of chlorophyll and the associated accessory pigments.…”

Section: Quality Of Lightmentioning

confidence: 99%

Modulations in Chlorophyll a Fluorescence Based on Intensity and Spectral Variations of Light

Janeeshma

Johnson

Amritha

et al. 2022

IJMS

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Photosynthetic efficiency is significantly affected by both qualitative and quantitative changes during light exposure. The properties of light have a profound effect on electron transport and energy absorption in photochemical reactions. In addition, fluctuations in light intensity and variations in the spectrum can lead to a decrease in photosystem II efficiency. These features necessitate the use of a simple and suitable tool called chlorophyll a fluorescence to study photosynthetic reactions as a function of the aforementioned variables. This research implies that chlorophyll a fluorescence data can be used to determine precise light conditions that help photoautotrophic organisms optimally function.

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“…Plant tolerance to UV is controlled by multiple genes [ 11 ]. By high-throughput RNA sequencing (RNA-seq), we found that 71,651 unigenes showed different expression patterns under UV-B stress in our transcriptome study of two species of R. chrysanthum , and the transcript abundance of most unigenes encoding cysteine and methionine metabolic pathways as well as glycine, serine and threonine metabolic pathways was reduced under UV-B stress.…”

Section: Introductionmentioning

confidence: 99%

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

Sun

Liu

Chen

et al. 2022

Plants

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Under natural environmental conditions, excess UV-B stress can cause serious injuries to plants. However, domestication conditions may allow the plant to better cope with the upcoming UV-B stress. The leaves of Rhododendron chrysanthum are an evergreen plant that grows at low temperatures and high altitudes in the Changbai Mountains, where the harsh ecological environment gives it different UV resistance properties. Metabolites in R. chrysanthum have a significant impact on UV-B resistance, but there are few studies on the dynamics of their material composition and gene expression levels. We used a combination of gas chromatography time-of-flight mass spectrometry and transcriptomics to analyze domesticated and undomesticated R. chrysanthum under UV-B radiation. A total of 404 metabolites were identified, of which amino acids were significantly higher and carbohydrates were significantly lower in domesticated R. chrysanthum. Transcript profiles throughout R. chrysanthum under UV-B were constructed and analyzed, with an emphasis on sugar and amino acid metabolism. The transcript levels of genes associated with sucrose and starch metabolism during UV-B resistance in R. chrysanthum showed a consistent trend with metabolite content, while amino acid metabolism was the opposite. We used metabolomics and transcriptomics approaches to obtain dynamic changes in metabolite and gene levels during UV-B resistance in R. chrysanthum. These results will provide some insights to elucidate the molecular mechanisms of UV tolerance in plants.

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Effect of high-intensity light and UV-B on photosynthetic activity and the expression of certain light-responsive genes in A. thaliana phyA and phyB mutants

Cited by 20 publications

References 37 publications

Influence of Light of Different Spectral Compositions on the Growth, Photosynthesis, and Expression of Light-Dependent Genes of Scots Pine Seedlings

Influence of Light of Different Spectral Compositions on the Growth, Photosynthesis, and Expression of Light-Dependent Genes of Scots Pine Seedlings

Modulations in Chlorophyll a Fluorescence Based on Intensity and Spectral Variations of Light

UV-B Irradiation to Amino Acids and Carbohydrate Metabolism in Rhododendron chrysanthum Leaves by Coupling Deep Transcriptome and Metabolome Analysis

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