Effect of High-fat Diet on Tracheal Responsiveness to Methacholine and  Insulin Resistance Index in Ovalbumin-sensitized Male and Female Rats

Ghobadi, Hassan; Alipour, Mohammad; Keyhanmanesh, ‬Rana; Boskabady, Mohammad Hossein; Aslani, Mohammad Reza

doi:10.18502/ijaai.v18i1.630

Cited by 11 publications

(11 citation statements)

References 46 publications

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“…The induction of asthma without changing the diet (AG) did not cause changes in weight or body mass of these animals when compared to CG (Table 2), indicating that asthma alone does not affect the weight of these animals. These dates are similar to the observed in other studies [11][12][13].…”

Section: Discussionsupporting

confidence: 92%

Functional and Morphologic Dysfunctions on Airways of Rats Submitted to an Experimental Model of Obesity-exacerbated Asthma

Ferreira

Pessoa

Figueiredo

et al. 2022

Preprint

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Obesity-exacerbated asthma phenotype is characterized by more severe asthma symptoms and glucocorticoid resistance. The aimed of this study is to standardize an obesity-exacerbated asthma model by high glycemic level index (HGLI) diet and ovalbumin (OVA) sensitization and challenges in Wistar rats. Animals were divided into groups: control (CG), obese (OG), asthmatic (AG), obese asthmatic (OAG) and obese asthmatic treated with dexamethasone (OADEXAG) and in vivo and in vitro functional and morphological parameters were measured. After HGLI consumption there was an increase in body weight, abdominal circumferences, body mass index, retroperitoneal, epididymal, inguinal adipose tissues and adiposity index. Respiratory function showed reduction in pulmonary tidal and minute-volume. In isolated trachea, the cumulative concentration-response curves for carbachol showed increase in contractile efficacy in OG, AG, OAG and OADEXAG, while OAG showed the greatest contractile efficacy. Histological sections of lungs showed an increased peribronchovascular inflammatory area on OAG, smooth muscle hypertrophy and remodeling area filled by extracellular matrix, and these changes were not reversed by treatment with dexamethasone. Obesity-exacerbated asthma model was successfully established. Therefore, this model allows further molecular investigations and the search for new therapeutic alternatives for the treatment and relief of symptoms of patients of obesity-induced resistant asthma.

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Section: Discussionsupporting

confidence: 92%

Functional and Morphologic Dysfunctions on Airways of Rats Submitted to an Experimental Model of Obesity-exacerbated Asthma

Ferreira

Pessoa

Figueiredo

et al. 2022

Preprint

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“…Our in vivo data are in keeping with previously published in vitro results showing that vitamin D improves the insulin signaling pathway and up-regulates the GLUT4 translocation (Manna and Jain, 2012), thus proving that both these effects are essential for maintenance of glucose metabolism. However, the beneficial effects of vitamin D in the improvement of lipid and glucose abnormalities proven by previous reports (McGill et al, 2008;Nagpal et al, 2009;Asemi et al, 2013;Ghobadi et al, 2019) further clarify the strong potential of this steroid vitamin in the treatment of numerous obesity-related disorders.…”

Section: Discussionmentioning

confidence: 88%

Histological, metabolic, and inflammatory changes in the renal tissues of high-fat diet-induced obese rats after vitamin D supplementation

Avestaei

Yaghchiyan

Alihemmati

et al. 2020

NFS

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Purpose Obesity is a major risk factor for chronic renal fibrosis and kidneys’ structural and inflammatory impairments. This study aims to examine the possible therapeutic effects of vitamin D supplementation against renal inflammatory and kidney’s structural fibrosis and degeneration. Design/methodology/approach Forty male Wistar rats were divided into two groups for 16 weeks: normal diet (ND) and high-fat diet (HFD); then, each group was subdivided into two groups including ND, ND + vitamin D and HFD, HFD + vitamin D. Vitamin D supplementation was done for five weeks at 500 IU/kg dosage. Renal tissue concentrations of tumor necrosis factor (TNF)-α, interleukin 6, interleukin 1 beta, monocyte chemoattractant protein (MCP)-1 and transforming growth factor-beta (TGF-β), serum values of lipids, markers of glucose homeostasis and urea, creatinine and uric acid and renal tissue histological and structural changes were determined. Findings HFD feeding caused remarkable histological and structural changes including higher TNF-α, MCP-1 and TGF-β concentrations in renal tissues of rats, whereas vitamin D has potent anti-inflammatory effects (P = 0.036, 0.047 and 0.02, respectively). Vitamin D administration also reduced urea and uric acid concentrations (P = 0.023 and 0.049, respectively). Moreover, vitamin D reduced glomerulomegaly, reduced lipid accumulation and limited dilated Bowman’s space in rats and improved glycemic status by increasing insulin (P = 0.04) and reducing insulin resistance (P = 0.006). Research limitations/implications The current study has some limitations. It was better to measure the level of inflammatory cytokines’ expression in the kidney tissues. Additionally, the measurement of baseline values of inflammatory cytokines was not possible because of the possibility of animals’ drop-out. Practical implications According to the study findings, vitamin D treatment in the current report showed a significant therapeutic role in reducing inflammation, improving glycemic and lipid abnormalities and structural and histological modifications in renal tissues of rats. These findings have a great value because after confirming in a human model, vitamin D can be suggested as a potential therapeutic tool in clinical practice. Social implications After being confirmed by other animal or human researches, the results of the current work could have great social implications by reducing the prevalence of obesity-related renal complications and highlighting the beneficial roles of vitamin D. Originality/value To the best of the authors’ knowledge, this is the first study to investigate the histological and inflammatory changes in the kidneys and metabolic parameters in the HFD induced rats and also clarified the therapeutic roles of vitamin D in ameliorating the inflammatory, histological, metabolic and functional changes in the kidneys of obese rats.

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“…ALT and AST was measured by quantitative detection kit (Pars Azmoon Co, Iran, sensitivities: 4 U.L and 2 U.L, respectively). To evaluate the insulin resistance, the following formula was used: Blood glucose (mg/dl) × fasting plasma insulin (IU mg/l in the fasting state) divided by 405 (23).…”

Section: Figure 1 Histopathology Results For Confirm Nafld Inductionmentioning

confidence: 99%

The Effect of Aerobic Continuous Training on Myonectin, Insulin Resistance and Liver Enzymes in Rats with Nonalcoholic Fatty Liver Disease

KoohestaniSini¹,

Afzalpour²,

Mohammadnia-Ahmadi³

et al. 2020

Ann Appl Sport Sci

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Background. Non-alcoholic fatty liver disease, is the most common reason of liver disorders that can be controlled by dieting and regular physical activity. Objectives. The aim of this study was to investigate the effect of aerobic continuous training on serum levels of myonectin, insulin resistance and liver enzymes in rats with non-alcoholic fatty liver disease. Methods. Thirty-four male Wistar rats (6-8 weeks old) were randomly assigned into two groups: normal diet (N = 9) and high-fat diet (N = 25). Through 12-week high-fat diet, induction of fatty liver was performed. In order to confirm non-alcoholic fatty liver induction, seven rats fed with high-fat diet were tested. The 18 remaining rats were randomly assigned to two groups: high-fat diet plus sedentary activity (n = 9) and high-fat diet plus aerobic continuous training (n = 9). The aerobic group has performed running on a treadmill at the intensity of 50-60% VO2max, for eight weeks and five sessions per week. The results were analyzed by one-way ANOVA and Tukey's post hoc tests at the statistical significance level of p<0.05. Results. The significant body weight increase induced by high-fat diet was controlled by aerobic continuous training (p<0.05). The serum levels of myonectin, insulin, glucose, alanine aminotransferase and aspartate aminotransferase enzymes and insulin resistance improved significantly after eight weeks of aerobic continuous training (p<0.05). Conclusion. It seems that aerobic continuous training can improve insulin resistance and liver enzymes by reducing serum myonectin levels in rats with non-alcoholic fatty liver disease.

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Effect of High-fat Diet on Tracheal Responsiveness to Methacholine and Insulin Resistance Index in Ovalbumin-sensitized Male and Female Rats

Cited by 11 publications

References 46 publications

Functional and Morphologic Dysfunctions on Airways of Rats Submitted to an Experimental Model of Obesity-exacerbated Asthma

Functional and Morphologic Dysfunctions on Airways of Rats Submitted to an Experimental Model of Obesity-exacerbated Asthma

Histological, metabolic, and inflammatory changes in the renal tissues of high-fat diet-induced obese rats after vitamin D supplementation

The Effect of Aerobic Continuous Training on Myonectin, Insulin Resistance and Liver Enzymes in Rats with Nonalcoholic Fatty Liver Disease

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