Effect of high carbohydrate diet on elongase and desaturase activity and accompanying gene expression in rat’s liver

Drąg, Jagoda; Goździalska, Anna; Knapik-Czajka, Małgorzata; Gawędzka, Anna; Gawlik, Katarzyna; Jaśkiewicz, Jerzy

doi:10.1186/s12263-017-0551-9

Cited by 20 publications

(14 citation statements)

References 38 publications

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“…SCD-1 activity was elevated in HCD mice, as reported by others in skeletal muscle (23). This higher activity leads to the generation of palmitoleic acid (16:1n-7), vaccenic acid (18:1n-7), and oleic acid (18:1n-9) (24).…”

Section: Discussionsupporting

confidence: 77%

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

Antunes

Godoy

Almeida-Souza

et al. 2020

Braz J Med Biol Res

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We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), D-6 desaturase (D6D), elongase 6, and de novo lipogenesis (DNL) were estimated. As for indicators of the inflammatory tissue state, we measured myeloperoxidase (MPO) activity and gene expression of F4/80, tumor necrosis factor-a (TNF-a), interleukin (IL)-4, IL-6, and IL-10. The HCD led to a lower deposition of SFA, MUFA, n-3 PUFA, and n-6 PUFA compared to HFD. However, the HCD increased arachidonic acid levels, SFA/n-3 PUFA ratio, DNL, SCD-1, D6D, and MPO activities, and expression of IL-6, contrasting with the general idea that increased lipid deposition is associated with more intense inflammation. The HCD was more potent to induce skeletal muscle inflammation than the HFD, regardless of the lower lipid accumulation.

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Section: Discussionsupporting

confidence: 77%

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

Antunes

Godoy

Almeida-Souza

et al. 2020

Braz J Med Biol Res

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“…Reverse transcriptase was from Invitrogen (Diego, CA, USA), thermal cycler was from Biometra (Goettingen, Germany, model: T1-Thermob lock), and electrophoresis apparatus and gel imager were from Bio-Rad (Hercules, CA, USA). The levels of FAS, SREBP1, and UCP2 were expressed relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 29 . The sequences of the primers used are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Nonylphenol aggravates non-alcoholic fatty liver disease in high sucrose-high fat diet-treated rats

Yang

et al. 2018

Sci Rep

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Exposure to environmental endocrine disruptors (EEDs) contributes to the pathogenesis of many metabolic disorders. Here, we have analyzed the effect of the EED-nonylphenol (NP) on the promotion of non-alcoholic fatty liver disease (NAFLD) in rats fed high sucrose-high fat diet (HSHFD). Fifty Sprague-Dawley rats were divided into five groups: controls fed a normal diet (C-ND); HSHFD-fed controls (C-HSHFD); and rats fed a HSHFD combined with NP at doses of 0.02 μg/kg/day (NP-L-HSHFD), 0.2 μg/kg/day (NP-M-HSHFD), and 2 μg/kg/day (NP-H-HSHFD). Subchronic exposure to NP coupled with HSHFD increased daily water and food intake (p < 0.05), hepatic echogenicity and oblique liver diameter (p < 0.05), and plasma levels of alanine aminotransferase, aspartate aminotransferase, total cholesterol, triglycerides, and low density lipoprotein cholesterol (p < 0.05). Combined exposure to NP and HSHFD induced macrovesicular steatosis with dilation and congestion of the central vein, liver inflammatory cell infiltration, and expression of genes regulating lipid metabolism, SREBP-1C, FAS, and Ucp2. These results demonstrate that NP aggravates NAFLD in HSHFD-treated rats by up-regulating lipogenic genes, and that HSHFD increases the toxic effects of NP. Thus subchronic NP exposure may lead to NAFLD, especially when combined with a high-sucrose/high-fat diet.

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“…Currently, there is no literature data showing the influence of oxidative stress, starvation, or nutritional deficiency on the expression of the discussed desaturases. It is only known that high-carbohydrate diets increase the protein expression of FADS2 (∆ 6 desaturase) in the rat liver but do not affect the protein expression of FADS1 (∆ 5 desaturase) [90]. Due to the lack of literature data, it is difficult to refer to the results of other studies.…”

Section: The Effect Of Necrotic Factors On the Expression Of Desaturamentioning

confidence: 99%

Expression of SCD and FADS2 Is Lower in the Necrotic Core and Growing Tumor Area than in the Peritumoral Area of Glioblastoma Multiforme

et al. 2020

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The expression of desaturases is higher in many types of cancer, and despite their recognized role in oncogenesis, there has been no research on the expression of desaturases in glioblastoma multiforme (GBM). Tumor tissue samples were collected during surgery from 28 patients (16 men and 12 women) diagnosed with GBM. The effect of necrotic conditions and nutritional deficiency (mimicking conditions in the studied tumor zones) was studied in an in vitro culture of human brain (glioblastoma astrocytoma) U-87 MG cells. Analysis of desaturase expression was made by qRT-PCR and the immunohistochemistry method. In the tumor, the expression of stearoyl–coenzyme A desaturase (SCD) and fatty acid desaturases 2 (FADS2) was lower than in the peritumoral area. The expression of other desaturases did not differ in between the distinguished zones. We found no differences in the expression of SCD, fatty acid desaturases 1 (FADS1), or FADS2 between the sexes. Necrotic conditions and nutritional deficiency increased the expression of the studied desaturase in human brain (glioblastoma astrocytoma) U-87 MG cells. The obtained results suggest that (i) biosynthesis of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) in a GBM tumor is less intense than in the peritumoral area; (ii) expressions of SCD, SCD5, FADS1, and FADS2 correlate with each other in the necrotic core, growing tumor area, and peritumoral area; (iii) expressions of desaturases in a GBM tumor do not differ between the sexes; and (iv) nutritional deficiency increases the biosynthesis of MUFA and PUFA in GBM cells.

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Effect of high carbohydrate diet on elongase and desaturase activity and accompanying gene expression in rat’s liver

Cited by 20 publications

References 38 publications

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle

Nonylphenol aggravates non-alcoholic fatty liver disease in high sucrose-high fat diet-treated rats

Expression of SCD and FADS2 Is Lower in the Necrotic Core and Growing Tumor Area than in the Peritumoral Area of Glioblastoma Multiforme

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