Effect of Heparin Supplementation into the Culture Medium on the Chromosomal Analysis of Bovine Blastocysts Derived from in vitro Fertilization

Abstract

Effects of the dose of heparin on the a, b: Values with diffe rent superscript letters were significantly different at the 5% level within c, d: Values with different superscript letters were significantly different at the 1% level within number of metaphase plates and on the sexing rates. for 2 h. SD). same stage. same stage. 713-717. 1972. 19) LARSON, R. C., G. G. IGNOTZ and W. B. CURRIE, Development of in vitro fertilized bovine embryos beyond the 8-cell block using completely defined medium containing fibronectin.

Required Concentration and Time of Vinblastine Treatment for Chromosome Preparation in Bovine Blastocysts Derived from In Vitro Fertilization.

“…The tendency of male advantage in diploid embryos compared with those found by some other researchers [17,20], although Kajihara et al [5] and Tokumaru et al [16] found a tendency toward This embryo was treated with vinblastine (100 ng/ ml) for 10 h. female advantage.…”

“…In investigations of chromosomes, with various mitotic inhibitors, many researchers have used short treatment times for bovine embryos fertilized in vitro. King et al [15] have described a method for preparing chromosomes from bovine blastocysts using 200 µg/ ml colchicine for 2 to 9.5 h. They have shown the mitotic indexes to be 14.7-37.5% in morphologically good blastocysts treated for 8.5-9.5 h, and 4.2-14.3% in embryos treated for 2-4 h. Kajihara et al [5] have reported frequencies of bovine embryos having metaphase plates of approximately 80-83%, and the sexing rate of 63.4% in embryos treated with 0.04 µg/ml colcemid for 1.5-3 h. Tokumaru et al [16] have also reported mitotic indexes of about 5-7% in bovine blastocysts treated with 0.04 µg/ml colcemid for 2 h. Avery et al [17] have shown a low mitotic index of 3.5%, in bovine in-vitro fertilized blastocysts treated with 0.01 µg/ml colcemid for 2 h. Iwasaki et al have reported mitotic indexes of approximately 5 to 12% in bovine blastocysts treated for 4 h with a combination of vinblastine sulfate and podophyllotoxin [7], and a mitoic index of approximately 26% in morulae or blastocysts treated for 6 h with 0.4 µg/ ml vinblastine sulfate [8]. If the treatment time is longer, the mitotic index is higher.…”