Effect of heavy ion-beam irradiation on plant growth and mutation induction in &lt;i&gt;Nicotiana tabacum&lt;/i&gt;

Kazama, Yusuke; Saito, Hiroyuki; Miyagai, Mashu; Takehisa, Hinako; Ichida, Hiroyuki; Miyazawa, Yutaka; Mishiba, Kei‐ichiro; Kanaya, Takeshi; Suzuki, Ken-ichi; Bae, Chang-Hyu; Miyoshi, K.; Mii, Masahiro; Abe, Tomoko

doi:10.5511/plantbiotechnology.25.105

Cited by 20 publications

(12 citation statements)

References 21 publications

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“…We have adopted several approaches to study the molecular nature of heavy‐ion beam‐induced mutations, including single‐gene analysis by PCR and sequencing (Kazama et al ., ; Hirano et al ., ) and genome‐wide analysis of large deletions by array‐comparative genomic hybridization (Kazama et al ., ; Hirano et al ., ) in the dicotyledonous model plant Arabidopsis ( Arabidopsis thaliana ). Carbon‐ion beam irradiation shows a high mutation efficiency (Kazama et al ., ), and mainly induces base substitutions and deletions and insertions of less than 100 bp (Kazama et al ., ). Whole‐genome sequencing (WGS) is a useful tool to analyze the genome‐wide nature of the induced mutations and the mutation frequency.…”

Section: Introductionmentioning

confidence: 99%

Targeted exome sequencing of unselected heavy‐ion beam‐irradiated populations reveals less‐biased mutation characteristics in the rice genome

et al. 2019

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SummaryHeavy‐ion beams have been widely utilized as a novel and effective mutagen for mutation breeding in diverse plant species, but the induced mutation spectrum is not fully understood at the genome scale. We describe the development of a multiplexed and cost‐efficient whole‐exome sequencing procedure in rice, and its application to characterize an unselected population of heavy‐ion beam‐induced mutations. The bioinformatics pipeline identified single‐nucleotide mutations as well as small and large (>63 kb) insertions and deletions, and showed good agreement with the results obtained with conventional polymerase chain reaction (PCR) and sequencing analyses. We applied the procedure to analyze the mutation spectrum induced by heavy‐ion beams at the population level. In total, 165 individual M2 lines derived from six irradiation conditions as well as eight pools from non‐irradiated ‘Nipponbare’ controls were sequenced using the newly established target exome sequencing procedure. The characteristics and distribution of carbon‐ion beam‐induced mutations were analyzed in the absence of bias introduced by visual mutant selections. The average (±SE) number of mutations within the target exon regions was 9.06 ± 0.37 induced by 150 Gy irradiation of dry seeds. The mutation frequency changed in parallel to the irradiation dose when dry seeds were irradiated. The total number of mutations detected by sequencing unselected M2 lines was correlated with the conventional mutation frequency determined by the occurrence of morphological mutants. Therefore, mutation frequency may be a good indicator for sequencing‐based determination of the optimal irradiation condition for induction of mutations.

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Section: Introductionmentioning

confidence: 99%

Targeted exome sequencing of unselected heavy‐ion beam‐irradiated populations reveals less‐biased mutation characteristics in the rice genome

et al. 2019

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“…Until now, it has been demonstrated in Arabidopsis that ion beams induce mutations with high frequency and show a broad mutation spectrum, and novel mutants have been obtained (Hase et al 2000, Tanaka et al 1997, Tanaka et al 2002. Furthermore, various mutants have been obtained in many crops, mainly ornamental plants (Hamatani et al 2001, Hara et al 2003, Kazama et al 2008, Miyazaki et al 2002, Miyazaki et al 2006, Nagatomi et al 1996, Okamura et al 2003, Yamaguchi et al 2003. Specific flower color mutants that could not be obtained by gamma rays, could be induced using ion beams, particularly in chrysanthemum (Nagatomi et al 1996) and carnation (Okamura et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Mutagenic effects of ion beam irradiation on rice

et al. 2009

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We investigated the usefulness of ion beams for mutation breeding in rice (Oryza sativa L.) by comparing the efficiency (i.e., the ratio of desirable mutations to plant damage such as lethality and sterility), mutation rate, spectrum, and optimum dose to that of gamma rays. Rice seeds were irradiated with carbon ions (mean linear energy transfer = 76 and 107 keV/µm), helium ions (9 keV/µm), and gamma rays, and their survival and fertility were examined in the M 1 generation. The frequency of chlorophyll mutations and their types (albina, xantha, and viridis) were examined in the M 2 generation, using the M 1 -plant progeny method. The efficiency of ion beams either equaled or exceeded that of gamma rays. In addition, the mutation rate of ion beams was higher than that of gamma rays. Thus, ion beams appeared to efficiently induce mutants with little radiation damage. No remarkable difference was observed in the relative frequencies of each type of mutation among 3 types of ion beams and gamma rays, thus suggesting that there was no difference in the spectrum. A shoulder dose, which hardly affected survival, was sufficient to efficiently obtain mutants for both types of radiation.

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“…Successful isolation of target mutants induced by heavy-ion beam irradiation has been reported for varieties of ornamental plants such as dahlia (Hamatani et al, 2001), petunia (Miyazaki et al, 2002), torenia (Miyazaki et al, 2006) and verbena Kanaya et al, 2008). This powerful technique has also been used to induce mutation in rice , Arabidopsis (Kazama et al, 2008b) and tobacco (Kazama et al, 2008a). Unlike gamma rays and X-rays, heavy-ion beams have high linear energy transfer (LET), which exerts stronger biological effects and can produce more DNA damage, leading to mutation and inactivation of single or multiple genes, inducing stable knockout mutants .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of an Arabidopsis Thaliana Self-Incompatibility Mutant Induced by Heavy-Ion Beam Irradiation

Lai¹,

Takehisa²

2013

Acta Biologica Cracoviensia Series Botanica

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Self-incompatibility (SI) is a genetic system that promotes outcrossing by rejecting self-pollen. In the Brassicaceae the SI response is mediated by the pistil S-locus receptor kinase (SRK) and its ligand, pollen Slocus cysteine-rich (SCR) protein. Transfer of SRK-SCR gene pairs to self-fertile Arabidopsis thaliana enabled establishment of robust SI, making this transgenic self-incompatible A. thaliana an excellent platform for SI analysis. Here we report isolation of a novel A. thaliana self-incompatibility mutant, AtC24 SI mutant, induced by heavy-ion beam irradiation. We show that the AtC24 SI mutant exhibits breakdown of SI, with pollen hydration, pollen tube growth and seed set resembling the corresponding processes in wild-type (self-fertile) A. thaliana. Further reciprocal crosses indicated that some perturbed SI factor in the stigmatic cell of the AtC24 SI mutant is responsible for the observed phenotype, while the pollen response remained intact. Our results demonstrate successful application of heavy-ion beam irradiation to induce a novel A. thaliana self-incompatibility mutant useful for SI studies.K Ke ey y w wo or rd ds s: : Arabidopsis thaliana accession C24, heavy-ion beam, pollen, pollen tube, self-incompatibility.

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Effect of heavy ion-beam irradiation on plant growth and mutation induction in <i>Nicotiana tabacum</i>

Cited by 20 publications

References 21 publications

Targeted exome sequencing of unselected heavy‐ion beam‐irradiated populations reveals less‐biased mutation characteristics in the rice genome

Targeted exome sequencing of unselected heavy‐ion beam‐irradiated populations reveals less‐biased mutation characteristics in the rice genome

Mutagenic effects of ion beam irradiation on rice

Isolation and Characterization of an Arabidopsis Thaliana Self-Incompatibility Mutant Induced by Heavy-Ion Beam Irradiation

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