Effect of Heat, Time and UV Light on Different Brands of Fluconazole by UV Spectrophotometeric Method

Naveed, Safila; Waheed, Nimra; Ashraf, Zehra; Nazeer, Safeena; Qamar, Fatima; Dilshad, Huma; Zafar, Farya; Ali, Huma

doi:10.14233/ajchem.2016.19434

Cited by 2 publications

(2 citation statements)

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“…cHG+abx eluate underwent liquid chromatography-mass spectrometry to determine the pharmacodynamic elution profile of the cHG+abx and potential antibiotic degradation occurring within the hydrogel, as antibiotic degradation is known to occur with changes to solution pH, temperature, or UV light exposure (Clindamycin, Fig 1C) [27,28]. No degradation of clindamycin or gentamicin was observed at any time point up to 72 hours.…”

Section: Lcms Elution Assaymentioning

confidence: 99%

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Jarman,

Burgess,

Sharma

et al. 2024

PLoS ONE

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The complexity of chronic wounds creates difficulty in effective treatments, leading to prolonged care and significant morbidity. Additionally, these wounds are incredibly prone to bacterial biofilm development, further complicating treatment. The current standard treatment of colonized superficial wounds, debridement with intermittent systemic antibiotics, can lead to systemic side-effects and often fails to directly target the bacterial biofilm. Furthermore, standard of care dressings do not directly provide adequate antimicrobial properties. This study aims to assess the capacity of human-derived collagen hydrogel to provide sustained antibiotic release to disrupt bacterial biofilms and decrease bacterial load while maintaining host cell viability and scaffold integrity. Human collagen harvested from flexor tendons underwent processing to yield a gellable liquid, and subsequently was combined with varying concentrations of gentamicin (50–500 mg/L) or clindamycin (10–100 mg/L). The elution kinetics of antibiotics from the hydrogel were analyzed using liquid chromatography-mass spectrometry. The gel was used to topically treat Methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium perfringens in established Kirby-Bauer and Crystal Violet models to assess the efficacy of bacterial inhibition. 2D mammalian cell monolayers were topically treated, and cell death was quantified to assess cytotoxicity. Bacteria-enhanced in vitro scratch assays were treated with antibiotic-embedded hydrogel and imaged over time to assess cell death and mobility. Collagen hydrogel embedded with antibiotics (cHG+abx) demonstrated sustained antibiotic release for up to 48 hours with successful inhibition of both MRSA and C. perfringens biofilms, while remaining bioactive up to 72 hours. Administration of cHG+abx with antibiotic concentrations up to 100X minimum inhibitory concentration was found to be non-toxic and facilitated mammalian cell migration in an in vitro scratch model. Collagen hydrogel is a promising pharmaceutical delivery vehicle that allows for safe, precise bacterial targeting for effective bacterial inhibition in a pro-regenerative scaffold.

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Section: Lcms Elution Assaymentioning

confidence: 99%

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Jarman,

Burgess,

Sharma

et al. 2024

PLoS ONE

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“…To obtain the appropriate wavelength for determination of fosfluconazole and the impurities, solutions of these compounds in the mobile phase were scanned by UV-visible spectrophotometry in the range 190-400 nm [11]. From the scanned results (Fig.…”

Section: The Selection Of Detection Wavelengthmentioning

confidence: 99%

Development and Validation of HPLC Method for Analysis of Impurities of Fosfluconazole in Pharmaceutical Products

Li¹,

Liu²,

Chen³

et al. 2016

BJPR

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Effect of Heat, Time and UV Light on Different Brands of Fluconazole by UV Spectrophotometeric Method

Cited by 2 publications

References 0 publications

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Development and Validation of HPLC Method for Analysis of Impurities of Fosfluconazole in Pharmaceutical Products

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