Effect of heat stress on DNA damage: a systematic literature review

Habibi, Peymaneh; Ostad, Seyed Naser; Heydari, Ahad; Aliebrahimi, Shima; Montazeri, Vahideh; Foroushani, Abbas Rahimi; Monazzam, Mohammad Reza; Ghazi‐Khansari, Mahmoud; Golbabaei, Farideh

doi:10.1007/s00484-022-02351-w

Cited by 14 publications

(10 citation statements)

References 57 publications

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“…Decreases in 8-OHdG with aging may be attributed to reduced energy metabolism, as suggested in humans and ungulates studies ( 65 , 67 ). For seasonality, elevated concentrations of 8-OHdG were found in elephants during the warmer months (March and May), aligning with studies in humans ( 68 – 70 ) showing higher 8-OHdG in summer to cope with high ambient temperature-induced oxidative stress. However, this contrasts with human studies in Netherlands ( 71 ) and China ( 72 ), which reported no seasonal effect on 8-OHdG, thus indicating possible environmental effects.…”

Section: Discussionsupporting

confidence: 81%

Seasonal patterns of oxidative stress markers in captive Asian elephants in Thailand and relationships to elephant endotheliotropic herpesvirus shedding

Kosaruk,

Brown,

Towiboon

et al. 2023

Front. Vet. Sci.

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IntroductionOxidative stress refers to an imbalance between oxidant and antioxidant activity and accumulation of reactive oxygen species, which can have detrimental effects on animal health. Annual fluctuations in oxidative stress status can occur, increasing disease susceptibility during certain time periods. However, a full understanding of factors related to oxidative stress in Asian elephants and how to mitigate the negative consequences is lacking.MethodsThis study measured six serum oxidative stress markers [reactive oxygen species (ROS), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), albumin, glutathione peroxidase (GPx), and catalase] and two stress markers [serum cortisol and fecal glucocorticoid metabolites (fGCM)] in 23 captive Asian elephants in Thailand over a 12 months period to examine relationships with age and season.ResultsSeasonal variations were observed, with several markers exhibiting significantly higher concentrations in the summer (ROS, MDA, 8-OHdG, albumin) and lower values during the rainy/winter seasons (MDA, 8-OHdG, albumin, catalase). By contrast, GPx was the only marker to be highest during the rainy season. For the stress markers, higher fGCM concentrations were noted during the rainy season, which contrasts with earlier studies showing more activity in the winter (tourist season). Positive correlations were found between the temperature-humidity index and ROS, GPx, and fGCM, while a negative correlation was observed with serum albumin. Elephant endotheliotropic herpesvirus (EEHV) shedding events were associated with higher concentrations of ROS and MDA. A moderate negative correlation was observed between 8-OHdG and the PCR threshold cycle of EEHV shedding (Ct), indicating DNA damage may be involved in EEHV shedding in elephants.DiscussionResults revealed significant age and seasonal effects on several oxidative stress markers, indicating those factors should be considered in study design and data interpretation. There also may be physiological adaptations in oxidative stress conditions in relation to environmental changes that could impact health outcomes.

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Section: Discussionsupporting

confidence: 81%

Seasonal patterns of oxidative stress markers in captive Asian elephants in Thailand and relationships to elephant endotheliotropic herpesvirus shedding

Kosaruk,

Brown,

Towiboon

et al. 2023

Front. Vet. Sci.

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“…According to previous studies, DNA damage, cell cycle disruption and cell death are increased in myoblasts exposed to oxidative stress [24,25]. Therefore, we first investigated whether fisetin could block H 2 O 2 -induced DNA damage by measuring DNA damage markers such as comet tail moment (DNA migration), p-γH2AX (Ser139) expression, and amount of 8-OHdG [26] and found that these three indicators were significantly increased in H 2 O 2 -treated C2C12 cells. However, all these changes were abrogated by fisetin pretreatment, and similar observations were made in NAC-pretreated cells.…”

Section: Discussionmentioning

confidence: 99%

Fisetin Protects C2C12 Mouse Myoblasts from Oxidative Stress-Induced Cytotoxicity through Regulation of the Nrf2/HO-1 Signaling

Park

Cha

Kim

et al. 2023

J. Microbiol. Biotechnol.

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IntroductionReactive oxygen species (ROS) are generally maintained at appropriate levels in the cells and act as regulators of signaling pathways involved in maintaining cellular homeostasis. However, excessive accumulation of ROS, defined as oxidative stress, causes irreversible oxidative damage to proteins, lipids, DNA and organelles, ultimately leading to apoptosis [1,2]. Therefore, it is necessary to strictly control the level of ROS through antioxidants or intracellular antioxidant systems to maintain normal cell function [3,4]. Recently, polyphenols, which are abundant in the plant kingdom, have been found to have various pharmacological effects, including inhibition of oxidative stress-dependent pathological conditions. Their antioxidant activity mainly involves ROS scavenging and activation of intracellular antioxidant signaling pathways, and can terminate oxidative damage-mediated responses before cells are severely affected for survival [5,6].Among polyphenols, fisetin, a flavonol molecule, has shown health-improving potential in numerous previous studies [7,8]. This phytochemical is found as a coloring component in fruits and vegetables such as apples, cucumbers, onions, and persimmons. According to accumulated studies, fisetin is known to have multiple pharmacological effects, including neuroprotective, antidiabetics, antiallergic, antithrombotic, senotherapeutic, Fisetin is a bioactive flavonol molecule and has been shown to have antioxidant potential, but its efficacy has not been fully validated. The aim of the present study was to investigate the protective efficacy of fisetin on C2C12 murine myoblastjdusts under hydrogen peroxide (H 2 O 2 )-induced oxidative damage. The results revealed that fisetin significantly weakened H 2 O 2 -induced cell viability inhibition and DNA damage while blocking reactive oxygen species (ROS) generation. Fisetin also significantly alleviated cell cycle arrest by H 2 O 2 treatment through by reversing the upregulation of p21WAF1/CIP1 expression and the downregulation of cyclin A and B levels. In addition, fisetin significantly blocked apoptosis induced by H 2 O 2 through increasing the Bcl-2/Bax ratio and attenuating mitochondrial damage, which was accompanied by inactivation of caspase-3 and suppression of poly(ADP-ribose) polymerase cleavage. Furthermore, fisetin-induced nuclear translocation and phosphorylation of Nrf2 were related to the increased expression and activation of heme oxygenase-1 (HO-1) in H 2 O 2stimulated C2C12 myoblasts. However, the protective efficacy of fisetin on H 2 O 2 -mediated cytotoxicity, including cell cycle arrest, apoptosis and mitochondrial dysfunction, were greatly offset when HO-1 activity was artificially inhibited. Therefore, our results indicate that fisetin as an Nrf2 activator effectively abrogated oxidative stress-mediated damage in C2C12 myoblasts.

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“…This resting quiescence-type phenotype can be described as an interphase of transient cell senescence of GCs [ 31 , 32 ]. The second reason pertains to the extreme stress and possible nuclear deformation, which also coincides with S and G2/M phase phenotype (though statistically significant but it cannot be construed as absolutely true) observed in our study can be best explained by the fact that extreme cellular stress can impact the attributes of nuclei during the G2/M phase [ 37 , 38 ], thus affecting the PI staining. For instance, when cells are in G2, they contain about twice the DNA content as in G1, which would normally mean they take up more dye and fluoresce more brightly.…”

Section: Discussionmentioning

confidence: 51%

Vitamin C Alleviates the Negative Effects of Heat Stress on Reproductive Processes by Regulating Amino Acid Metabolism in Granulosa Cells

Sammad,

Ahmed,

Ullah

et al. 2024

Antioxidants

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Heat stress-induced biochemical alterations in ovarian follicles compromise the function of granulosa cells (GCs) and the developmental competence of oocytes. Summer heat stress can have a far-reaching negative impact on overall fertility and reproductive success. Together with the heat stress, the rise of assisted reproductive technologies (ART), potential confounding hazards of in vitro handling and the absence of systemic body support in ART makes it imperative to study the heat stress ameliorative effects of vitamin C under in vitro conditions. Using in vitro heat stress treatment of 43 °C for two hours in bovine GCs, we studied the effects of vitamin C on cell growth, oxidative stress, apoptosis and cell cycle progression together with a comprehensive metabolomics profiling. This study investigates the molecular milieu underlying the vitamin C (VC)-led alleviation of heat-related disruptions to metabolic processes in bovine GCs. The supplementation of VC ameliorated the detrimental effects of heat stress by reducing oxidative stress and apoptosis while restoring cell proliferation. Normal cell function restoration in treated GCs was demonstrated through the finding of significantly high levels of progesterone. We observed a shift in the metabolome from biosynthesis to catabolism, mostly dominated by the metabolism of amino acids (decreased tryptophan, methionine and tyrosine) and the active TCA cycle through increased Succinic acid. The Glutathione and tryptophan metabolism were important in ameliorating the inflammation and metabolism nexus under heat stress. Two significant enzymes were identified, namely tryptophan 2,3-dioxygenase (TDO2) and mitochondrial phenylalanyl-tRNA synthetase (FARS2). Furthermore, our findings provide insight into the significance of B-complex vitamins in the context of heat stress during VC supplementation. This study underscores the importance of VC supplementation in heat stress and designates multiple metabolic intervention faucets in the context of ameliorating heat stress and enhancing reproductive efficiency.

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Effect of heat stress on DNA damage: a systematic literature review

Cited by 14 publications

References 57 publications

Seasonal patterns of oxidative stress markers in captive Asian elephants in Thailand and relationships to elephant endotheliotropic herpesvirus shedding

Seasonal patterns of oxidative stress markers in captive Asian elephants in Thailand and relationships to elephant endotheliotropic herpesvirus shedding

Fisetin Protects C2C12 Mouse Myoblasts from Oxidative Stress-Induced Cytotoxicity through Regulation of the Nrf2/HO-1 Signaling

Vitamin C Alleviates the Negative Effects of Heat Stress on Reproductive Processes by Regulating Amino Acid Metabolism in Granulosa Cells

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