Effect of Heat Stress on 2-Hydroxy-4-(Methylthio)Butanoic Acid and DL-Methionine Absorption Measured In Vitro

Dibner, J. J.; Atwell, C. A.; Ivey, F. J.

doi:10.3382/ps.0711900

Cited by 37 publications

(24 citation statements)

References 44 publications

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“…Thus, HS exposure may result in impaired digestibility of various essential amino acids. For example, decreased L-leucine transport has been reported across broilers' intestinal epithelium in in vitro HS studies (11). Accelerated replacement of enterocytes requires energy and proteins, which can negatively impact the growth and development of other tissues and organ systems (12).…”

Section: Introductionmentioning

confidence: 99%

The effects of dietary glutamine supplementation on growth performance and intestinal morphology of broiler chickens reared under hot conditions

JAZIDEH¹,

Farhoomand²,

Daneshyar³

et al. 2014

Turk J Vet Anim Sci

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Section: Introductionmentioning

confidence: 99%

The effects of dietary glutamine supplementation on growth performance and intestinal morphology of broiler chickens reared under hot conditions

JAZIDEH¹,

Farhoomand²,

Daneshyar³

et al. 2014

Turk J Vet Anim Sci

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“…While differences in absorption, metabolism, organ distribution and/or clearance between HMB and DLM (Saunderson 1985(Saunderson , 1987Dibner 2003) may also play a role, changes in the efficacy of either source of Met to fulfill the birds' requirements for total sulfur amino acids during HS cannot be discounted. Dibner et al (1992) and Knight et al (1994) measured the absorption of DLM in vitro as affected by HS in broilers. These authors concluded that the intestinal uptake of the D isomer of methionine was negatively impaired during HS mainly due to a significant reduction in the energy-and Na-dependent pathway.…”

Section: Discussionmentioning

confidence: 99%

“…In a subsequent experiment, these authors observed even greater growth depression in heat-stressed birds fed diets supplemented with D-methionine vs. those with L-Met suggesting that the impaired absorption for D-methionine mediated the lower performance of broilers fed DLM under tropical environments. However, the effects of HS on the in vitro uptake of these Met sources found by Dibner et al (1992) and Knight et al (1994) correspond to situations of short exposures to high temperatures and these effects have not been evaluated in vivo or in birds subjected to prolonged HS ( > 2 wk).…”

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confidence: 99%

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Effect of arginine:lysine and methionine source on amino acid digestibility and response to dietary electrolytes in chronically or acutely heat-stressed broilers

González-Esquerra¹,

Leeson²

2006

Can. J. Anim. Sci.

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Gonzalez-Esquerra, R. and Leeson, S. 2006. Effect of arginine:lysine and methionine source on amino acid digestibility and response to dietary electrolytes in chronically or acutely heat-stressed broilers. Can. J. Anim. Sci. 86: 263-271. In two experiments, the effects of arginine:lysine, NaHCO 3 and methionine source on performance and amino acid digestion of heat-stressed broilers were studied. In exp. 1, corn/soy diets with argine:lysine (Arg:Lys) of 1.10, 1.25 and 1.40 were fed from 21 to 42 d. Diets were supplemented with 2-hydroxy-4-(methylthio)-butanoic acid (HMB) or DL-Methionine (DLM) with NaHCO 3 at 0 or 1.04%. Ten replicates per treatment of four birds per cage were maintained at 31.7 ± 0.9°C. From 21 to 28 d weight gain (BWG), feed consumption and feed-to-gain (FCR) were unaffected by diet treatment. Methionine source did not influence bird performance (P > 0.05). However, from 35 to 42 d, when diets were supplemented with NaHCO 3 , BWG and FCR of birds fed DLM were compromised when offered diets with Arg:Lys at 1.40. In diets devoid of NaHCO 3 , increasing Arg:Lys to 1.25 impaired BWG and FCR for broilers fed DLM, although effects disappeared when more Arg was used. Performance of birds on HMB was mostly unaffected by Arg:Lys or NaHCO 3 except when fed diets void of NaHCO 3 with Arg:Lys of 1.40, when FCR was impaired. In exp. 2, broilers were maintained at thermoneutrality (TN) reaching 20°C at 33 d. Other birds were at 31°C throughout, representing chronic heat stress (CHS), while a third group changed suddenly from 20 to 31°C at 25 d representing acute heat stress (AHS). Birds within each environment were housed in one of two rooms with eight replicates of four birds per treatment. At 25 d, birds were fed Arg:Lys of 0.95 or 1.40 with DLM supplemented at requirement. Birds fed Arg:Lys of 1.40 were also offered diets with synthetic L-methionine (L-Met). Adding L-Met to high Arg diets during CHS significantly depressed ileal digestibility of all amino acids (P < 0.05). It is concluded that chronic heat stress can affect the digestibility of amino acids, and that this effect may impact performance.Key words: Broilers, methionine, arginine, heat stress, digestibility, sodium bicarbonate Gonzalez-Esquerra, R. et Leeson, S. 2006. Incidence de la source d'arginine:lysine sur la digestibilité des acides aminés et réaction aux électrolytes des aliments chez les poulets de chair soumis à un stress thermique chronique ou aigu. Can. J. Anim. Sci. 86: 263-271. Les auteurs ont imaginé deux expériences pour vérifier l'incidence de la source d'arginine:lysine, du NaHCO 3 et de la méthionine sur la digestion des acides aminés et le rendement des poulets de chair soumis à un stress thermique. Dans le cadre de la première expérience, ils ont servi une ration de maïs et de soja contenant un ratio d'arginine:lysine (Arg:Lys) de 1,10, 1,25 ou 1,40 aux volatiles du 21 e au 42 e jour. La ration était enrichie d'acide 2-hydroxy-4-(méthylthio)-butanoïque (HMB) ou de DL-méthionine (DLM) assortie de NaHCO 3 à 0 ou à 1,04 %. L...

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“…For years, several models have been employed in attempts to reveal the absorption mechanisms for Met and/or HMB in chickens (1)(2)(3)(4)(5)(6)(7). These studies suggested that mechanisms of methionine absorption included Na ϩ -dependent transport, Na ϩ -independent transport and/or diffusion.…”

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confidence: 99%

Poly(A)+ RNA Encoding Proteins Capable of Transporting l-Methionine and/or dl-2-Hydroxy-4-(Methylthio) Butanoic Acid Are Present in the Intestinal Mucosa of Broilers

Pan

Wong

Dibner

et al. 2002

The Journal of Nutrition

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To investigate the presence of poly (A) ϩ RNA that encode proteins capable of transporting Lmethionine (L-Met) and/or DL-2-hydroxy-4-(methylthio) butanoic acid (HMB), Xenopus oocytes were injected with poly(A) ϩ RNA isolated from broiler intestinal mucosa. Healthy oocytes at stage V or VI were collected from Xenopus laevis and microinjected with water, poly(A) ϩ RNA or size-fractioned poly(A) ϩ RNA. The ability of the injected oocytes to take up either L-Met or HMB was examined by incubating oocytes with [methyl-3 H]-L-Met or [5-14 C]-HMB. A greater uptake of L-Met (P Ͻ 0.01) and HMB (P Ͻ 0.05) by oocytes injected with poly(A) ϩ RNA from the duodenum, jejunum and ileum of the small intestine was observed compared with water-injected oocytes. The greatest (P Ͻ 0.05) uptake occurred when poly(A) ϩ RNA from the jejunum or ileum was injected. Injections from four different pools of sucrose gradient-fractionated poly (A) ϩ RNA from all three intestinal segments induced (P Ͻ 0.01) L-Met uptake. There were three to four different pools of sucrose gradient-fractionated poly(A) ϩ RNA from the duodenum, jejunum and ileum that induced (P Ͻ 0.05) HMB uptake. Uptake of HMB was greater at pH 5.5 than at pH 7.5 and was independent of Na ϩ . Uptake of L-Met induced by all four poly(A) ϩ RNA pools decreased dramatically when Na ϩ was removed from the uptake buffer, which indicated that the majority of L-Met uptake was Na ϩ -dependent. Methionine and DL-2-hydroxy-4-(methylthio) butanoic acid (HMB) are commonly used as supplements in diet formulation in poultry feeding because of insufficient quantities of Met in commercial feedstuffs. For years, several models have been employed in attempts to reveal the absorption mechanisms for Met and/or HMB in chickens (1-7). These studies suggested that mechanisms of methionine absorption included Na ϩ -dependent transport, Na ϩ -independent transport and/or diffusion. Mechanisms suggested for HMB absorption included Na ϩ -independent but H ϩ -dependent transport, and/or diffusion. However, because of the inherent characteristics of the models used in the studies, the actual presence of mRNA that encode proteins capable of Met or HMB uptake or the presence of specific transport proteins for these substrates in chicken intestine has not been described.The application of molecular biotechnology to nutrition research has provided tools for researchers attempting to resolve the nature of potential absorption mechanisms. One of the unique techniques that has resolved many questions related to transport proteins is expression cloning using Xenopus laevis oocytes. Xenopus oocytes are a robust system for the expression of many different proteins of animal or plant origin (8). The Xenopus oocyte is a cell specialized for the production and storage of proteins for later use during embryogenesis. Xenopus oocytes at stage V have the complete machinery to translate exogenous RNA faithfully. The most important aspect is that the oocyte expression system can make posttranslational modification...

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Effect of Heat Stress on 2-Hydroxy-4-(Methylthio)Butanoic Acid and DL-Methionine Absorption Measured In Vitro

Cited by 37 publications

References 44 publications

The effects of dietary glutamine supplementation on growth performance and intestinal morphology of broiler chickens reared under hot conditions

The effects of dietary glutamine supplementation on growth performance and intestinal morphology of broiler chickens reared under hot conditions

Effect of arginine:lysine and methionine source on amino acid digestibility and response to dietary electrolytes in chronically or acutely heat-stressed broilers

Poly(A)+ RNA Encoding Proteins Capable of Transporting l-Methionine and/or dl-2-Hydroxy-4-(Methylthio) Butanoic Acid Are Present in the Intestinal Mucosa of Broilers

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