Effect of heat stress on peroxidase activity and total protein content in strawberry plants

Gülen, Hatice; Eri̇ş, A.

doi:10.1016/j.plantsci.2003.11.014

Cited by 168 publications

(94 citation statements)

References 26 publications

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“…The elevation of temperature resulted in increase in EL indicating membrane injury, which is reported to be a direct consequence of high temperature (Coria et al 1998). The EL has been reported as a useful indicator of heat stress injury to plants in some earlier studies (Liu and Huang 2000;Gulen and Eris 2004) and our findings in this context match with the similar ones reported in heat-stressed strawberry (Gulen and Eris 2004) and rice (Sohn and Back 2007) plants. The cellular respiration was assessed using 2,3,5 triphenyl tetrazolium chloride (TTC) reduction assay that indicates cellular respiration.…”

Section: Effects Of Heat Stresssupporting

confidence: 90%

Proline induces heat tolerance in chickpea (Cicer arietinum L.) plants by protecting vital enzymes of carbon and antioxidative metabolism

Kaushal

Gupta

Bhandhari

et al. 2011

Physiol Mol Biol Plants

156

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Chickpea is a heat sensitive crop hence its potential yield is considerably reduced under high temperatures exceeding 35°C. In the present study, we evaluated the efficacy of proline in countering the damage caused by heat stress to growth and to enzymes of carbon and antioxidative metabolism in chickpea. The chickpea seeds were raised without (control) and with proline (10 μM) at temperatures of 30/25°C, 35/30°C, 40/35°C and 45/40°C as day/ night (12 h/12 h) in a growth chamber. The shoot and root length at 40/35°C decreased by 46 and 37 %, respectively over control while at 45/40°C, a decrease of 63 and 47 %, respectively over control was observed. In the plants growing in the presence of 10 μM proline at 40/35°C and 45/40°C, the shoot length showed improvement of 32 and 53 %, respectively over untreated plants, while the root growth was improved by 22 and 26 %, respectively. The stress injury (as membrane damage) increased with elevation of temperatures while cellular respiration, chlorophyll content and relative leaf water content reduced as the temperature increased to 45/40°C. The endogenous proline was elevated to 46 μmol g −1 dw at 40/35°C but declined to 19 μmol g −1 dw in plants growing at 45/40°C that was associated with considerable inhibition of growth at this temperature. The oxidative damage measured as malondialdehyde and hydrogen peroxide content increased manifolds in heat stressed plants coupled with inhibition in the activities of enzymatic (superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase) and levels of non-enzymatic (ascorbic acid, glutathione, proline) antioxidants. The enzymes associated with carbon fixation (RUBISCO), sucrose synthesis (sucrose phosphate synthase) and sucrose hydrolysis (invertase) were strongly inhibited at 45/40°C. The plants growing in the presence of proline accumulated proline up to 63 μmol g −1 dw and showed less injury to membranes, had improved content of chlorophyll and water, especially at 45/40°C. Additionally, the oxidative injury was significantly reduced coupled with elevated levels of enzymatic and non-enzymatic antioxidants. A significant improvement was also noticed in the activities of enzymes of carbon metabolism in proline-treated plants. We report here that proline imparts partial heat tolerance to chickpea's growth by reducing the cellular injury and protection of some vital enzymes related to carbon and oxidative metabolism and exogenous application of proline appears to have a countering effect against elevated high temperatures on chickpea.

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Section: Effects Of Heat Stresssupporting

confidence: 90%

Proline induces heat tolerance in chickpea (Cicer arietinum L.) plants by protecting vital enzymes of carbon and antioxidative metabolism

Kaushal

Gupta

Bhandhari

et al. 2011

Physiol Mol Biol Plants

156

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“…invasion of pathogen) as well as abiotic stress (e.g. heat-shock) has been confirmed (Baker and Orlandi 1995;Bolwell and Wojtaszek 1997;Gulen and Eris 2004). Peroxidases (POXs) represent one of the important groups of enzymes, which participate in the metabolism of ROS in plants (Fig.…”

Section: Involvement and Activity Of Antioxidant Enzymes In Plant Sysmentioning

confidence: 93%

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

et al. 2013

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Tomato powdery mildew (Oidium neolycopersici) is one of the most devastating diseases of cultivated tomatoes worldwide. Although the first epidemics were recorded more than 25 years ago many aspects of this host-pathogen interaction are still not well understood. Detailed morphological and molecular studies of the anamorphs confirmed that O. neolycopersici is phylogeneticaly close to Erysiphe aquilegiae var. ranunculi. Host range is rather broad, apart from Solanaceae hosts were found in the families Apocynaceae, Campanulaceae, Crassulaceae, Cistaceae, Cucurbitaceae, Linaceae, Malvaceae, Papaveraceae, Pedialiaceae, Scrophulariaceae, Valerianaceae a Violaceae. Non-host resistance within these families is not based on inhibition of formation of primary haustorium, however, on post-haustorial hypersensitive reponse and another type of non-hypersensitive resistance. Screening of wild Solanum species (previous Lycopersicon spp.) germplasm revealed valuable sources of resistance (S. habrochaites, S. pennellii, S. cheesmaniae, S. chilense, S. peruvianum). The main resistance mechanism was found to be a hypersensitive response (HR), in some cases followed by limited development of the pathogen. However, there is a broad variation in resistance response on the histological and cytological level. Interaction between many wild Solanum spp. and O. neolycopersici is race-specific, at least three races were differentiated. In some interspecific crosses (S. lycopersicum × S. habrochaites) adult plant resistance was observed. Biochemical studies focusing on production of reactive oxygen species (ROS) and peroxidase activity during infection of O. neolycopersici showed that production of ROS and activity of corresponding enzymes is related to activation of defence responses in genotypes of wild Solanum sect. Lycopersicon. The significance of nitric oxide (NO) in O. neolycopersici pathogenesis was supported by experiments with NO donors and scavengers. In moderately resistant genotype S. chmielewskii, treatment by heat stress caused slight deceleration of pathogen development, increased production of jasmonic acid (JA) and abscisic acid (ABA) and increased peroxidase activity in infected plants. The different degree of tomato resistance/ susceptibility did not markedly change the rate and extent of photosynthetic response to O. neolycopersici; only minimal impairment of photosynthesis was found in both susceptible and moderately resistant genotypes during the Harper Adams University, Newport, Shropshire TF10 8NB, UK first 9 days after inoculation. The accumulated evidence confirm a crucial role of localised increased production of ROS and reactive nitrogen species (RNS) in response to pathogen penetration into plant tissue and its involvement in the plant resistance responses including the initiation and progression of plant cell death in host wild Solanum species. Crucial points of further research are discussed.

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“…There are a number of publications demonstrating irreversible damage to the plants caused by dangerously low or high temperatures (Chaitanya et al, 2001;Gulen and Eris, 2004;Wahid et al, 2007). For most plants, the threshold temperatures range from 30 to 40°C.…”

Section: Introductionmentioning

confidence: 99%

“…For most plants, the threshold temperatures range from 30 to 40°C. A decrease in the protein content was shown for seedlings of strawberry exposed to gradual heat stress (from 25 to 45°C) and shock heat stress (45°C without adaptation) accompanied by an increase in total peroxidase activity at high temperature treatment (Gulen and Eris, 2004). Heat stress was also reported to induce or enhance active oxygen species-scavenging enzymes like SOD, catalase, peroxidase, and several low-molecular antioxidant compounds (Chaitanya et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Effect of temperature on oxidative stress induced by lead in the leaves of Plantago major L.

Balakhnina

Borkowska

Nosalewicz

et al. 2016

International Agrophysics

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A b s t r a c t. Fluctuation of the summer day-time temperatures in the mid-latitudes in a range from 16 to 30 o C should not have irreversible negative effects on plants, but may influence metabolic processes including the oxidative stress. To test the effect of moderately high temperature on oxidative stress induced by lead in the leaves of Plantago major L.; the plants were incubated in a water solution of 0, 150, 450, and 900 µM Pb(NO 3 ) 2 at 20 and 28 o C. Plant reactions were evaluated by the content of thiobarbituric acid reactive substances and ascorbate peroxidase and glutathione reductase activities in leaves after 2, 24, 48, and 72 h. The Pb concentration in the leaves rose with the increase in the Pb content and was higher at 20 o C. The increase in stomatal resistance caused by Pb was higher at 28 o C. The contents of TBARS increased after 2 h of plant exposure to Pb and the increase was the highest at 900 µM Pb, 28 o C. The AsP activity increased up to 50% after 24 h of Pb-treatment at 28 o C; the highest increase in glutathione reductase activity was observed after 72 h at 20 o C. Thus, the moderately high temperature 28 o C compared with optimal 20 o C caused a decrease in Pb accumulation in Plantago leaves but amplified the negative effects of lead, especially in the beginning of stress development.K e y w o r d s: ascorbate peroxidase, glutathione reductase, lead, heat stress, oxidative destruction, Plantago major L.

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Effect of heat stress on peroxidase activity and total protein content in strawberry plants

Cited by 168 publications

References 26 publications

Proline induces heat tolerance in chickpea (Cicer arietinum L.) plants by protecting vital enzymes of carbon and antioxidative metabolism

Proline induces heat tolerance in chickpea (Cicer arietinum L.) plants by protecting vital enzymes of carbon and antioxidative metabolism

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

Effect of temperature on oxidative stress induced by lead in the leaves of Plantago major L.

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