Effect of heat shock on function of frozen/thawed bull spermatozoa

Monterroso, Victor; Drury, K.C.; Ealy, Alan D.; Edwards, J. L.; Hansen, P. J.

doi:10.1016/0093-691x(95)00282-d

Cited by 24 publications

(19 citation statements)

References 47 publications

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“…Even short-term aging can compromise sperm function. In this study, motility of bull sperm was decreased by 4 h incubation at 38.5 C and slightly more so by incubation at 40 C. Previous work indicates bull sperm motility was decreased by as little as 3 h incubation at 39 C [22]. The reduction in cleavage rate when oocytes were fertilized with sperm aged at 38.5 C or 40 C in the current study could reflect decreased sperm motility and fertilizing capacity.…”

Section: Discussionmentioning

confidence: 44%

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Consequences for the Bovine Embryo of Being Derived from a Spermatozoon Subjected to Post-Ejaculatory Aging and Heat Shock: Development to the Blastocyst Stage and Sex Ratio

2009

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Abstract. The objective was to determine whether aging of sperm caused by incubation at normothermic (38.5 C) or heat shock (40 C) temperatures for 4 h prior to oocyte insemination affects sperm motility, fertilizing ability, competence of the resultant embryo to develop to the blastocyst stage and blastocyst sex ratio. In the first experiment, the percent of sperm that were motile was reduced by aging (P<0.001) and the reduction in motility was greater for sperm at 40 C compared to sperm at 38.5 C (P<0.01). In the second experiment, oocytes were inseminated with aged sperm. A smaller percent of oocytes fertilized with sperm aged at either temperature cleaved by Day 3 after insemination than oocytes fertilized with fresh sperm (P<0.05). There was no effect of sperm aging on the percent of oocytes or cleaved embryos that developed to the blastocyst stage. Aging of sperm before fertilization at 38.5 C reduced the percent of blastocysts that were male (P=0.08). In the third experiment, incubation of sperm at 38.5 C or 40 C for 4 h did not reduce fertilizing ability of sperm as determined by pronuclear formation at 18 h post insemination. In conclusion, aging of sperm reduced cleavage rate and the percent of blastocysts that were males but had no effect on the developmental capacity of the embryo. The effect of aging on cleavage rate may represent reduced motility and errors occurring after fertilization and pronuclear formation. Aging at a temperature characteristic of maternal hyperthermia had little additional effect except that polyspermy was reduced. Results indicate that embryo competence for development to the blastocyst stage is independent of sperm damage as a result of aging for 4 h at normothermic or hyperthermic temperatures. Key words: Aging, Bovine, Fertilization, Heat shock, Spermatozoa (J. Reprod. Dev. 55: [69][70][71][72][73][74] 2009) evelopmental competence of the mammalian embryo is dependent on genetic and non-genetic contributions from its parents [1][2][3][4][5][6]. Sperm could affect an embryo's competence for development if the timing of fertilization or early cleavage is delayed. For example, embryos produced by high-fertility bulls entered S-phase of the first cell cycle earlier and had a longer S-phase than those produced by low-fertility bulls [7]. In another study, spermatozoa from 50% of bulls identified as being of low fertility in artificial insemination studs experienced premature capacitation [8]. Damage to the macromolecular portions of the sperm that are incorporated by the embryo could also result in formation of embryos with reduced developmental competence. Among these sperm contributions are DNA, the centriole [9], and RNA [10]. Embryos fertilized with semen containing a high proportion of sperm with extensive DNA damage have reduced competence for development [11][12][13] but the importance of damage to the centriole or sperm RNA is not known.Damage to sperm can occur in the male reproductive tract or after deposition of sperm in the female. In bulls, for example, thermal...

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Section: Discussionmentioning

confidence: 44%

“…The term 'aging' was used to represent incubation in vitro since this treatment can cause a reduction in sperm motility after 3 h [22]. A second hypothesis was that sperm damage would be enhanced if aging occurred at elevated temperatures.…”

mentioning

confidence: 99%

Consequences for the Bovine Embryo of Being Derived from a Spermatozoon Subjected to Post-Ejaculatory Aging and Heat Shock: Development to the Blastocyst Stage and Sex Ratio

2009

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“…testicular environment, spermatozoa are, in fact, vulnerable to sudden temperature changes. Even incubation of spermatozoa at normothermic temperatures (398C) for a short period of time (3 h) compromises their viability and functional competence (Monterroso et al 1995). In the present study, both total and progressive sperm motility, as well as other parameters of sperm velocity determined by CASA, decreased after spermatozoa were incubated for 4 h at 38.58C, with even worse effects seen after incubation at 418C.…”

Section: Discussionmentioning

confidence: 60%

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Rahman

Vandaele²,

Rijsselaere

et al. 2014

Reprod. Fertil. Dev.

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Abstract. Heat stress has long been recognised as a cause of subfertility in farm animals. The objectives of the present study were to elucidate the effect of heat stress on sperm function and involvement of the mitogen-activated protein kinase (MAPK) 14 signalling pathway. Spermatozoa incubated for 4 h at a physiological temperature (38.58C) exhibited significantly (P , 0.05) reduced motility, plasma membrane integrity and mitochondrial potential compared with nonincubated spermatozoa; the reductions in these parameters were more severe following incubation at a hyperthermic (418C) temperature (P , 0.01). Percentages of fertilisation and embryo development were highly affected in spermatozoa incubated at 418C compared with non-incubated spermatozoa (P , 0.01). Similarly, embryo quality was adversely affected by sperm incubation at 418C, as indicated by a higher apoptotic cell ratio in Day 7 blastocysts compared with that in the non-incubated control group (14.6% vs 6.7%, respectively; P , 0.01). Using SB203580 (10 mg mL À1 ), a specific inhibitor of the p38 MAPK pathway, during sperm hyperthermia reduced MAPK14 activation (24.9% vs 35.6%), increased sperm motility (45.8% vs 26.5%) and reduced DNA fragmentation (16.9% vs 23.4%) compared with the untreated control group, but did not improve subsequent fertilisation and embryo development. In conclusion, heat stress significantly affects the potential of spermatozoa to penetrate oocytes, as well as subsequent embryo development and quality. Notably, the data show that the MAPK14 signalling pathway is largely involved in heat-induced sperm damage. However, further research is needed to elucidate other signalling pathways possibly involved in heat-induced sperm damage.Additional keywords: functional parameters, mitogen-activated protein kinase 14 phosphorylation, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling.

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“…Thermal stress before insemination has been associated with reduced fertility [3,32]. In addition, it has been reported that heat stress during the summer season reduced bovine sperm motility [33]. Heat stress during IVF also inhibited the subsequent development of bovine embryos [34,35].…”

Section: Discussionmentioning

confidence: 99%

“…However, the groups exposed to any class of mTHI on day 0 before AI did not show reduced conception rates in this study. Also, heat stress within the physiologic range (41-42 C) did not influence bovine sperm functions such as fertilizability, DNA integrity or sperm head acrosome reactions in vitro [33]. Putney et al [36] reported that when heat stress affected superovulated Holstein heifers for 10 h from the onset of estrus, embryonic development, but not fertilization, was inhibited (the number of degenerated or retarded embryos increased).…”

Section: Discussionmentioning

confidence: 99%

Effect of the Temperature-Humidity Index on Body Temperature and Conception Rate of Lactating Dairy Cows in Southwestern Japan

et al. 2011

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Abstract. In the present study, we investigated the relationship between the temperature-humidity index (THI) and the conception rate of lactating dairy cows in southwestern Japan, one of the hottest areas of the country. We also investigated the relationship between measurement of the vaginal temperature of lactating dairy cows as their core body temperature at one-hour intervals for 25 consecutive days in hot (August-September, n=6) and cool (JanuaryFebruary, n=5) periods and their THI. Furthermore, we discussed the above relationship using these vaginal temperatures, the conception rates and the THI. As a result, when the conception rates from day 2 to 0 before AI were classified into day 2, 1 and 0 groups by the six maximum THI values in each group (mTHI; <61,(61)(62)(63)(64)(65)(66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79)(80) >80), only the conception rate for the mTHI over 80 at 1 day before AI group was significantly lower (P<0.05) than the other groups. The conception rate for days 15 to 17, but not days 19 to 22 and 30 to 35, after AI in the cows that experienced average mTHI over 80 (amTHI>80) was significantly lower (P<0.05) than that of the cows that did not experience amTHI>80. There was a significant positive correlation (P<0.01) between the mTHI and the mean daily vaginal temperature, but not during the cool period. When the mTHI reached 69, the vaginal temperature started to increase. As for the relationship between the conception rates and vaginal temperatures for all mTHI classes, in the mTHI>80 at 1 day before AI group, the vaginal temperature increased by 0.6 C from 38.7 C, resulting in a reduction of 11.6% in the conception rate from 40.5%. In conclusion, these results suggest that one of the causes of the fall in conception rate of lactating dairy cows during the summer season in southwestern Japan may be an increase in their core body temperature with a higher mTHI than the critical mTHI of 69 at 1 day before AI. Key words: Conception rate, Lactating dairy cow, Temperature-humidity index (THI), Vaginal temperature (J. Reprod. Dev. 57: [450][451][452][453][454][455][456] 2011) eat stress is any combination of environmental parameters producing conditions that are higher than the temperature range of an animal's thermal neutral zone [1]. Heat stress can have major effects on most aspects of reproductive function in mammals. These include disruptions in spermatogenesis and oocyte development, oocyte maturation, early embryonic development, fetal and placental growth and lactation [2]. For lactating dairy cows, heat stress during the summer season suppresses the conception rate compared with other seasons [3]. This has been observed clearly in higher milk-producing dairy cows [3][4][5][6]. As the average production per cow has drastically increased [7,8] (doubled over the last two decades), the metabolic heat output per animal has increased substantially, rendering animals more susceptible to heat stress [9][10][11][12]. Consequently, the trend in the decline in the reproductive...

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Effect of heat shock on function of frozen/thawed bull spermatozoa

Cited by 24 publications

References 47 publications

Consequences for the Bovine Embryo of Being Derived from a Spermatozoon Subjected to Post-Ejaculatory Aging and Heat Shock: Development to the Blastocyst Stage and Sex Ratio

Consequences for the Bovine Embryo of Being Derived from a Spermatozoon Subjected to Post-Ejaculatory Aging and Heat Shock: Development to the Blastocyst Stage and Sex Ratio

Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

Effect of the Temperature-Humidity Index on Body Temperature and Conception Rate of Lactating Dairy Cows in Southwestern Japan

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