Effect of heat load and dietary protein on oxygen pulse and energy cost for locomotion in heifers

Abstract

The objectives of the present study were to evaluate the effect of heat load, bodyweight and dietary protein on oxygen pulse of heifers, to obtain the energy cost of walking on flat and sloping terrain, and to compare the energy cost of heifers during continuous walking and intermittent walking. In Experiment 1, the correlations of oxygen consumption, heart rate and oxygen pulse (O2P) with bodyweight, black globe temperature and temperature and humidity index were examined. Moreover, the effect of dietary protein on O2P was evaluated. The temperature and humidity index and black globe temperature had a low positive correlation with O2P and oxygen consumption, and a low negative correlation with heart rate. However, weight had no correlation with O2P. There was a linear increase in O2P with a very low adjustment with an increasing dietary crude protein concentration. In Experiment 2, the energy cost of heifers walking continuously at a constant speed in a terrain with 0%, 6% and 12% gradient was measured. The energy expenditure was similar among the terrain gradients. The heifers walking had a 16.6% higher energy expenditure than when they were standing. In Experiment 3, a comparison of the energy cost was made among heifers standing, continuously walking and intermittently walking at a constant speed on flat ground. The energy cost for walking was similar between continuous and intermittent walking. The heat load, bodyweight and dietary protein concentration had a low effect on O2P in dairy heifers. Therefore, measurements over a short time (5–15 min) are a reliable estimator of O2P through the day. Both intermittent and continuous walking can be used to evaluate energy expenditure.