Effect of Heat-Inactivated Kefir-Isolated Lactobacillus kefiranofaciens M1 on Preventing an Allergic Airway Response in Mice

Abstract

In this study, we assessed the anti-asthmatic effects of heat-inactivated Lactobacillus kefiranofaciens M1 (HI-M1) and its fermented milk using different feeding procedures and at various dosage levels. The possible mechanisms whereby HI-M1 has anti-allergic asthmatic effects were also evaluated. Ovalbumin (OVA)-allergic asthma mice that have been orally administrated the HI-M1 samples showed strong inhibition of production of T helper cell (Th) 2 cytokines, pro-inflammatory cytokines, and Th17 cytokines in splenocytes and bronchoalveolar fluid compared to control mice. An increase in regulatory T cell population in splenocytes in the allergic asthma mice after oral administration of H1-M1 was also observed. In addition, all of the features of the asthmatic phenotype, including specific IgE production, airway inflammation, and development of airway hyperresponsiveness, were depressed in a dose-dependent manner by treatment. These findings support the possibility that oral feeding of H1-M1 may be an effective way of alleviating asthmatic symptoms in humans.

Impact of Kefir DerivedLactobacillus kefirion the Mucosal Immune Response and Gut Microbiota

“…However, features regarding the effects of bacteria remain very important. It has been recently described that one strain of L. kefiranofaciens protects mice in a model of allergy [20] and also in an experimental model of colitis [19], but to our knowledge, our work constitutes the first report of the in vivo immunomodulatory activity of L. kefiri .…”

“…Among kefir yeasts, Kluyveromyces marxianus CIDCA 8154 and Saccharomyces cerevisiae CIDCA 8112 downregulate intestinal epithelial innate response through a mechanism dependent on NF-kB modulation [18]. In the case of lactic acid bacteria retrieved from kefir, L. kefiranofaciens has been proven to ameliorate colitis in a DSS-induced murine model [19] and to produce antiasthmatic effects on ovalbumin-allergic asthma mice [20]. On the other hand, Carey and Kostrzynska [21] showed that L. kefiri attenuates the proinflammatory response in intestinal epithelial cells induced by Salmonella Typhimurium and Hong et al [22] showed its influence on Th1 and proinflammatory cytokines production on macrophages.…”

Oral Administration of Heat-Inactivated Lactobacillus plantarum K37 Modulated Airway Hyperresponsiveness in Ovalbumin-Sensitized BALB/c Mice

“…To evaluate the anti-allergy effect of K37, the 6-week-old mice were sensitized and challenged with OVA to establish an OVA-induced airway allergy BALB/c mice model according to a published report [16] with slight modifications. The experimental procedure for administration of K37, OVA immunization, and sample collection is summarized in Figure 1.…”

Effect of the Velvet Antler of Formosan Sambar Deer (Cervus unicolor swinhoei) on the Prevention of an Allergic Airway Response in Mice

“…The ovalbumin- (OVA-) sensitized mouse model of allergic airway inflammation was used in this study and has been described in detail by Lee et al [23] and Hong et al [24]. The mice were fed with 10 mg of VA powder/mouse/day throughout the experimental period.…”

“…The preparation of the splenocyte cultures followed the procedure described by Hong et al [24]. Briefly, The spleens were removed and placed in 10 mL of RPMI-1640 medium (HyClone, Logan, UT, USA) supplemented with 1% antibiotics (50  μ g/mL penicillin, 50  μ g/mL streptomycin sulfate, and 100  μ g/mL neomycin sulfate; Invitrogen, Carlsbad, CA, USA).…”