Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and a temperate pig breed

Bambou, Jean-Christophe; Grondin, Roxanne; Gourdine, Jean-Luc; Renaudeau, David

doi:10.1017/s2040470010000476

Cited by 1 publication

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“…One of the most compelling findings in our study was that the expression of heat shock protein 70 (HSP70) had differentially expressed transcripts during the two period. All animals respond to heat stress at the cellular level by synthesizing HSPs, which help protect cells from heat-induced injury (Bambou et al 2011). A recent study suggest that HSP70 may improve thermo tolerance in pigs (Chen et al 2005), and the level of HSP correlated with heat-tolerant cattle breeds (Kamwanja et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of the hypothalamus during prelaying and laying periods in Sichuan white geese (Anser cygnoides)

Gao

Zhao

et al. 2015

Animal Science Journal

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The Sichuan White goose is a Chinese breed well known for the quality of its meat. However, reproductive performance in this goose is not ideal, and little information is available regarding the abundance of transcripts. To better understand the molecular mechanism(s) underpinning prelaying and laying periods in the Sichuan white goose, high-throughput RNA sequencing was performed to analyze the transcriptome in the hypothalamus. After sequencing and annotation, 26,921 unigenes were obtained, with 48 transcripts up-regulated in the prelaying period and 180 transcripts up-regulated during the laying period. These transcripts were primarily related to diseases, cancers, signaling molecules and interactions, the nervous system and the immune system. Eight transcripts were selected for further analyses with quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The qRT-PCR results were mostly consistent with those from the high-throughput RNA sequencing. Among these transcripts, serine/threonine-protein kinase (AMPK), heat shock protein 70 (HSP70) and NADH dehydrogenase 1 (ND1) were differentially expressed during the prelaying and laying periods. The results of this study provide a useful resource for future studies examining the hypothalamus in geese.

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Section: Discussionmentioning

confidence: 99%