EFFECT OF HCG ANTISERUM ON OVULATION AND CORPUS LUTEUM FORMATION IN THE MONKEY (MACACA FASCICULARIS)¹

Control of the Menstrual Cycle

“…336,337 The requirement for LH in the control of progesterone secretion and the functional life span of the corpus luteum has been the subject of numerous debates. An obligatory requirement for LH in the maintenance of the corpus luteum was indicated when Moudgal et al 254 passively immunized rhesus monkeys with antibodies against LH and found that immunized animals exhibited premature luteal regression, an observation later confirmed by others. Subsequent studies by Vande Wiele et al 30 demonstrated that the long half-life of hCG used to induce ovulation provided extended gonadotropic support to the corpus luteum; when LH was used to induce ovulation, the corpus luteum regressed after 5-6 days unless additional LH was given throughout the luteal phase.…”

Examination of the role of FSH in periovulatory events in the hamster

“…While FSH has been shown to bring about ovulation in hypophysectomized, pentobarbital-or chlorpromazine-blocked rats and in PMSG-primed immature rats (Carter et al, 1961 ;Lostroh & Johnson, 1966;Harrington, Bex, Elton & Roach, 1970;Nuti, McShan & Meyer, 1974;Tsafriri et al, 1976), endogenous FSH has not been found necessary for ovulation in any studies, including the present, in which FSH antiserum was used (Schwartz, Krone, Talley & Ely, 1973;Moudgal, Rao, Maneckjee, Muralidhar, Mukku & Sheela Rani, 1974;Jagannadha Rao et al, 1974a;Schwartz, Cobbs, Talley & Ely, 1975). The role of LH in ovulation, on the other hand, has been unequivocally demonstrated in many systems (Kelly, Robertson & Stanfield, 1963;Lostroh & Johnson, 1966;Schwartz & Gold, 1967;Sasamoto, 1969;Madhwa Raj & Moudgal, 1970;Ely & Schwartz, 1971;Moudgal, MacDonald & Greep, 1971;Jagannadha Rao et al, 1974a;Lipner et al, 1974;Schwartz et al, 1975;Tsafriri et al, 1976). It is probable that in systems in which ovulation has been induced by FSH, it is due to synergism with the minimal amounts of LH that may be found in the system (either present in the system ia low amounts, even after hypophysectomy, or as a contaminant), because FSH in combination with minimal amounts of LH has been shown to cause ovulation in hypophysectomized immature rats (Lipner et al, 1974).…”

Role of Endogenous Primate LH in Maintaining Corpus Luteum Function of the Monkey

“…1 Visiting Associate Professor of Anatomy. Permanent Address: Department of Biochemistry, Indian Institute of Science, Bangalore 12, India.…”

“…The conclusion drawn from these observations is that LH has an important role in the maintenance of the continued function of the subhuman primate corpus luteum. (/ Clin Endocrinol Metab 35: 113, 1972) I N A recent communication, we observed that administration of an hCG antiserum (A/S) to cycling monkeys from days 10 through 13 blocked ovulation and corpus luteum formation as judged by shortening of the menstrual cycle, gross observation of the ovary at laparotomy, and failure of the concentration of plasma progesterone to rise (1). In the present report we present immunochemical and biological evidence for the cross-reactivity of this hCG A/S with monkey LH.…”