Effect of Gymnema sylvestre R. Br. leaves extract on certain physiological parameters of diabetic rats

Shafey, Aziza A.M. El; El-Ezabi, Magda M.; Seliem, Moshira M.E.; Ouda, H; Ibrahim, Doaa S.

doi:10.1016/j.jksus.2012.11.001

Cited by 47 publications

(28 citation statements)

References 30 publications

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“…This result is consistent with previous studies that have indicated that Gs may possess antidiabetic properties (26,27). Numerous mechanisms have been proposed to explain the apparent antidiabetic activity of Gs extract.…”

Section: Discussionsupporting

confidence: 94%

Neuroprotective effects of Gymnema sylvestre on streptozotocin-induced diabetic neuropathy in rats

Fatani

Al‐Rejaie

Abuohashish

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The application of traditional medicine for diabetes and associated complications, such as diabetic neuropathy (DN), has received increasing attention. The aim of the present study was to investigate the potential ameliorative effect of Gymnema sylvestre (Gs) in a rat model of DN. Diabetes was induced via a single intraperitoneal injection of streptozotocin (STZ; 60 mg/kg). Treatment with Gs extract (50 or 100 mg/kg/day) began two weeks following the administration of STZ and was continued for five weeks. Pain threshold behavior tests were performed subsequent to the five-week Gs treatment period. In addition, the serum levels of glucose, insulin and proinflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6, were determined. Furthermore, the sciatic tissue levels of nitric oxide, thiobarbituric acid reactive substances and reduced glutathione were determined, as well as the activity levels of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. Levels of insulin-like growth factor (IGF), nerve growth factor (NGF), TNF-α, IL-1β and IL-6 were also assessed in the sciatic tissue. In addition, the sciatic nerve tissue samples were analyzed for histopathological alterations. The diabetic rats exhibited apparent reductions in the paw-withdrawal (31%; P<0.01) and tail-flick latencies (38%; P<0.05). Furthermore, the diabetic rats demonstrated an evident elevation in serum and sciatic levels of proinflammatory cytokines. Measured oxidative stress biomarkers were significantly altered in the sciatic nerve tissue of the diabetic rats. Treatment with Gs attenuated diabetes-induced modifications with regard to the levels of serum glucose, insulin and proinflammatory cytokines. In the sciatic nerve tissue, the diabetes-induced alterations in IL levels and oxidative stress biomarkers were significantly improved in the Gs-treated rats. Furthermore, the reduction in the sciatic tissue expression levels of IGF and NGF was also ameliorated by Gs treatment. Histological analysis indicated that Gs corrected the sciatic tissue in the diabetic rats. Therefore, the results demonstrated that the neuroprotective effect of Gs may be associated with the inhibitory effect on the excessive activation of inflammatory molecules and oxidative stress mediators.

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Section: Discussionsupporting

confidence: 94%

Neuroprotective effects of Gymnema sylvestre on streptozotocin-induced diabetic neuropathy in rats

Fatani

Al‐Rejaie

Abuohashish

et al. 2015

Experimental and Therapeutic Medicine

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“…However, there might be a positive role of RC-extract on weight gain and energy metabolism. Similar effects of RC-extract for their possible hypoglycaemic potential on rats have been reported previously (Daisy et al 2009;El Shafey et al 2013). Lipid profile results showed that the RC-extract might have positive effects on lipid metabolism.…”

Section: Discussionsupporting

confidence: 85%

“…There is increasing evidence that complications associated with diabetes may be related to oxidative stress induced by the production of free radicals. Several studies have shown elevated MDA levels in the diabetic state (Daisy et al 2009;El Shafey et al 2013). This escalation indicates a significant rise in ROS.…”

Section: Discussionmentioning

confidence: 97%

Healing effects of sumac (Rhus coriaria) in streptozotocin-induced diabetic rats

Doğan

Çelik

2016

Pharmaceutical Biology

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Context Sumac [Rhus coriaria L. (RC) (Anacardiaceae)] is used as a folk medicine in the treatment of diabetes in Turkey. Objective This study investigates the in vivo healing and protective effects of lyophilized extract sumac against streptozotocin (STZ)-induced diabetic complications. Materials and methods Toxicity test was conducted in three different dosages (250, 500 and 1000 mg/kg of plant extracts, respectively). Six groups of seven rats each were used in experiments. Groups were designed as Normal control, Diabetic (DM), DM + AC-20 mg/kg, DM + Extract-100 mg/ kg, DM + Extract 250 mg/kg and DM + Extract 500 mg/kg group. Experimental diabetes [50 mg/kg, intraperitoneal (i.p.)] was induced by STZ. The effects of oral administration of the extract for 21 d on the level of serum glucose, insulin, C-peptide, lipid profile (LP), hepatic and renal damage biomarkers (HRDB), diabetic serum biomarkers (DSB), glycosylated haemoglobin (HbA1c), antioxidant defence system constituents (ADSCs), malondialdehyde (MDA) and a-glucosidase activity in small intestine tissue were evaluated. Results The extract decreased the levels of blood glucose in diabetic groups (an average of 31%). Triglyceride, total cholesterol, high-density lipoprotein and low-density lipoprotein levels were balanced by plant extract (500 mg/kg) supplementation in the diabetic group. Decreased levels of aspartate aminotransferase (89%), alanine aminotransferase (91%), lactate dehydrogenase (35%), alkaline phosphatase (47%), creatinine (25%) and urea (29%) were detected in plant extract (500 mg/kg) supplemented diabetic group. Additionally, a considerable increase in the HRDB, DSB, LP, MDA and fluctuated ADSC levels were restored in RC-extract supplemented groups. Conclusion RC lyophilized extract has a healing effect on diabetes and diabetes-related complications.ARTICLE HISTORY

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“…23 Already many researchers have reported that the leaves of G. sylvestre lower blood sugar, stimulate the heart, uterus, and circulatory systems, and exhibit anti-sweet and hepatoprotective activities. 20,[24][25][26][27][28][29][30][31] Administration of G. sylvestre extract to diabetic rats increased superoxide dismutase activity and decreased lipid peroxide by either directly scavenging the reactive oxygen species, due to the presence of various antioxidant compounds, or by increasing the synthesis of antioxidant molecules (albumin and uric acid). 24,30,32 …”

Section: Introductionmentioning

confidence: 99%

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Arunachalam¹,

Arun²,

Annamalai³

et al. 2014

IJN

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Background Gymnema sylvestre is an ethno-pharmacologically important medicinal plant used in many polyherbal formulations for its potential health benefits. Silver nanoparticles (SNPs) were biofunctionalized using aqueous leaf extracts of G. sylvestre . The anticancer properties of the bioactive compounds and the biofunctionalized SNPs were compared using the HT29 human adenoma colon cancer cell line. Methods The preliminary phytochemical screening for bioactive compounds from aqueous extracts revealed the presence of alkaloids, triterpenes, flavonoids, steroids, and saponins. Biofunctionalized SNPs were synthesized using silver nitrate and characterized by ultraviolet–visible spectroscopy, scanning electron microscopy, energy-dispersive X-ray analysis, Fourier transform infrared spectroscopy, and X-ray diffraction for size and shape. The characterized biofunctionalized G. sylvestre were tested for its in vitro anticancer activity against HT29 human colon adenocarcinoma cells. Results The biofunctionlized G. sylvestre SNPs showed the surface plasmon resonance band at 430 nm. The scanning electron microscopy images showed the presence of spherical nanoparticles of various sizes, which were further determined using the Scherrer equation. In vitro cytotoxic activity of the biofunctionalized green-synthesized SNPs (GSNPs) indicated that the sensitivity of HT29 human colon adenocarcinoma cells for cytotoxic drugs is higher than that of Vero cell line for the same cytotoxic agents and also higher than the bioactive compound of the aqueous extract. Conclusion Our results show that the anticancer properties of the bioactive compounds of G. sylvestre can be enhanced through biofunctionalizing the SNPs using the bioactive compounds present in the plant extract without compromising their medicinal properties.

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Effect of Gymnema sylvestre R. Br. leaves extract on certain physiological parameters of diabetic rats

Cited by 47 publications

References 30 publications

Neuroprotective effects of Gymnema sylvestre on streptozotocin-induced diabetic neuropathy in rats

Neuroprotective effects of Gymnema sylvestre on streptozotocin-induced diabetic neuropathy in rats

Healing effects of sumac (Rhus coriaria) in streptozotocin-induced diabetic rats

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

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