Effect of gut bacterial isolates from Apis mellifera jemenitica on Paenibacillus larvae infected bee larvae

Abstract

The probiotic effects of seven newly isolated gut bacteria, from the indegenous honey bees of Saudi Arabia were investigated. bioassays were used to investigate the effects of each gut bacterium namely, (T1), (T2), (T3), (T4), (T5), (T6), and (T7) on mortality percentage of honey bee larvae infected with spores along with negative control (normal diet) and positive control (normal diet spiked with spores). Addition of gut bacteria to the normal diet significantly reduced the mortality percentage of the treated groups. Mortality percentage in all treated groups ranged from 56.67% up to 86.67%. T6 treated group exhibited the highest mortality (86.67%), whereas T4 group showed the lowest mortality (56.67%). Among the seven gut bacterial treatments, T4 and T3 decreased the mortality 56.67% and 66.67%, respectively, whereas, for T2, T6, and T7 the mortality percentage was equal to that of the positive control (86.67%). Mortality percentages in infected larval groups treated with T1, and T5 were 78.33% and 73.33% respectively. Most of the mortality occurred in the treated larvae during days 2 and 3. Treatments T3 and T4 treatments showed positive effects and reduced mortality.

Physicochemical Analysis and Essential Oils Extraction of the Gorse (Ulex europaeus) and French Broom (Genista monspessulana), Two Highly Invasive Species in the Colombian Andes

“…Another important compound is acetic acid, which was found in both species. This can be used in agriculture in the control of larvae and the eggs of the wax moths, since it destroys the wax combs [59,60]. Other studies suggest useful effects against pathogens in a variety of food products such as moutable (eggplant dip) [61,62], salads [63,64], hummus [65], and tahini [66,67].…”

The Effect of Pesticides on the Microbiome of Animals

“…The probiotic effects of seven isolated intestinal bacteria species from Apis mellifera jemenitica to larvae of a bee infected with Paenibacillus were studied. The following intestinal bacteria were used in the experiment: Fructobacillus fructosus, Proteus mirabilis, Bacillus licheniformis, Lactobacillus kunkeei, Bacillus subtilis, and Enterobacter kobei, Morganella morganii [114]. It turned out that adding these probiotic microorganisms to the diet significantly reduces the mortality rate of honey bee larvae.…”

Effect of Lactic Bacteria on <i>Ascosphaera apis</i> and <i>A. atra </i>

“…melliventris and L. helsingborgensis were isolated from bee gut and stomach [15] but they were not mentioned as beneficial. L. kunkei reduced the mortality of bee larvae infected with Paenibacillus larvae [1].The antimycotic effects produced by lactic bacteria include organic acids, cyclic dipeptides, proteinaceous compounds, and others [7].…”