Effect of Guava (Psidium guajava) Leaves Essential Oil on Some Reproductive Parameters in Male Guinea Pig (Cavia porcellus)

Abstract

Aim: To assess the effects of essential oïl from guava leaves on some reproductive parameters.Methods: Thirty-two male guinea pigs (Cavia porcelus) of three months old and weighing between 350 and 400 g were used. These guinea pigs were divided into 4 groups of 8 animals each. Each group was randomly attributed orally for 60 days one of the following guava leaf essential oil doses: 0 (control group), 80, 100 and 120 μl of oil per kg body weight. At the end of treatment, all animals were sacrificed to assess the reproductive parameters.Results: Body weight, relative weight of the seminal vesicles, vas deferens, and epididymis were not significantly increased (p>0.05) with the considered guava leaves essential oil doses. The testes weights significantly increased (p<0.05) in animals who received 100 and 120 μl/kg body weight compared to control. A significant increase (p<0.05) in sperm concentration, mobility and viability was observed in animals which received the essential oil doses compared to control. Also, the level of proteins and testosterone in serum and testes significantly increased (p<0.05) with the essential oil doses. Conclusion:Guava leaves essential oil can be used to improve male reproductive performances.

Guinea Pig Sperm Morphology and Fertility under Different Photoperiod

“…Guinea pig spermiogram studies show variable concentrations and motility rates depending on the method of extraction and analysis. In spermatozoa from the epididymis, as in this study, Ayala Guanga et al [21] reported 418.0 × 10 6 /mL and 58% motility, Rodríguez et al [22] reported 95% motility, and Ferdinand et al [23] reported 149.85 × 10 6 /mL sperm concentration. Using electroejaculation, the values are generally low, ranging from 36.70 to 47.33 × 10 6 /mL for concentration and 49.49 to 90.86% for individual motility [14,24,25].…”

Spermatozoa Characteristics, Serum Metabolites and Testicular Oxidative Stress Traits in Guinea Pigs (Cavia porcellus) Fed on Zanthoxyliun leprieurii Fruit

“…In rats treated with aqueous extracts of Psidium guajava leaves (100, 200 and 300 mg/kg bw) [25,26] and in male guinea pigs treated with essential oil of Psidium guajava leaves (80, 100 and 120 μl /kg bw), [29] the similar results were also recorded. This observation might be explained by the action of antioxidant compounds such as phenols, flavonoids, tannins, terpenoids and saponins revealed in this extract following the phytochemical tests [27]. These molecules could have neutralized free radical attacks [28] or inhibiting enzymes responsible of their production and then protecting cells against oxidative stress.…”