Effect of Growth Regulators on Cell Growth and Flavonoid Production in Cell Culture of Elaecarpus grandiflorus

Abstract

Elaecarpus grandiflorus has the potential to be developed as a source of bioactive compounds. This study aims to obtain the most optimal medium for cell culture induction and flavonoid production in Elaeocarpus grandiflorus culture. Picloram (3.5, 5 and 7.5 ppm) and 2, 4-D (1.5, 2.5 and 3.5 ppm) were used for induction of cell suspension culture. Cell suspension culture induction was observed through growth parameters (fresh and dry weight of cells) and cell suspension formation. In addition, it was found the production of flavonoids. Induction of cell culture is done by growing callus on Woody Plant Medium (WPM) with a variety of growth regulators. The culture was maintained in a shaker at a speed of 120 rpm for 30 days. At harvest, cells were filtered, weighed and dried. The spectrophotometer was used to determine the total flavonoid content. Quercetin was used as a standard compound. The best cell culture induction was obtained in cells maintained in WPM medium with the addition of 2, 4-D 2, 5 ppm. All cells in various treatment media can produce flavonoids with varying concentrations. WPM medium with the addition of 2, 4 D and Picloram can be used for the production of flavonoids from Elaecarpus grandiflorus cells.

Induksi Kalus Eksplan Daun Porang (Amorphophallus muelleri Blume) Menggunakan Kombinasi Air Kelapa dan IAA (Indole Acetic Acid)

“…Keberhasilan perbanyakan tanaman melalui kultur in vitro, salah satunya dipengaruhi oleh penambahan zat pengatur tumbuh (ZPT) dan senyawa organik kompleks ke dalam media (Habibah et al, 2019). ZPT berperan penting dalam mengatur arah regenerasi eksplan membentuk kalus, tunas atau akar.…”

“…ZPT berperan penting dalam mengatur arah regenerasi eksplan membentuk kalus, tunas atau akar. Regenerasi eksplan dipengaruhi oleh jenis dan rasio konsentrasi ZPT meliputi auksin dan sitokinin (Ferziana et al, 2021;Habibah et al, 2019). Salah satu jenis auksin yang umum digunakan dalam menginduksi kalus adalah IAA (Indole Acetic Acid).…”

LC-MS Based Secondary Metabolites Profile of Elaeocarpus grandiflorus J.E. Smith. Cell Suspension Culture Using Picloram and 2,4-Dichlorophenoxyacetic Acid

“…The explants were collected from young petiole leaves 3-5 from the shoots. The petioles were managed aseptically, then sterilized using a fungicide, bactericide, and 5.25% NaClO solution following the procedure reported by Habibah et al 12,14 The petiole pieces were placed on a WPM agar medium and incubated at 26°C for five months in dark conditions. The produced calluses were used as a material for the induction of cell culture.…”

Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

“…Ex situ conservation attempts using in vitro culture can be used to effectively propagate this plant. Several studies related to in vitro culture of Kepel plants have been conducted with several focuses, including optimization of sterilization methods for leaf explants (Habibah et al, 2013), nodes (Hutabarat et al, 2022) and endosperm (Handayani et al, 2022), optimization of PGR that supports callus growth of Kepel seed and fruit mesocarp explants (Habibah et al, 2016(Habibah et al, , 2017(Habibah et al, , 2019, and nodal explant (Sekar et al, 2023), and optimization of flavonoid production from callus and cell suspension cultures of Kepel plants (Habibah et al, 2016(Habibah et al, , 2019. However, in vitro propagation of Kepel plants still faces obstacles, including the browning of explants.…”