Effect of growth regulators on transformed root cultures of Hyoscyamus muticus

Vanhala, Leena; Eeva, Manu; Lapinjoki, Seppo; Hiltunen, R.; Oksman-Caldentey, Kirsi-Marja

doi:10.1016/s0176-1617(98)80177-6

Cited by 43 publications

(16 citation statements)

References 22 publications

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“…The authors also ascertained that the most effective concentration for biomass production in this culture was NAA 10 lmol l À1 , and IAA 25 lmol l À1 . However, Vanhala et al (1998) has observed that IAA and NAA did not significantly affect the biomass production in Hyoscyamus culture in 12-h photoperiod. It can be assumed that as light conditions so plant species significantly contribute to the final effect of certain auxin on root biomass production.…”

Section: Resultsmentioning

confidence: 93%

“…Various growth regulators influence in different way the root induction, elongation, and biomass production (Gyulai et al, 1993;Biondi et al, 1997;Vanhala et al, 1998;Ba´lva´nyos et al, 2001;Balestri and Bertini, 2003).…”

Section: Resultsmentioning

confidence: 99%

“…Addition of e.g. IAA or NAA into the culture medium in 12-h photoperiod increased biomass production in Lobelia (Ba´lva´nyos et al, 2001), or was without significant effect in Hyoscyamus culture (Vanhala et al, 1998). IAA, IBA, and NAA promoted the elongation of tomato root in continuous light.…”

Section: Introductionmentioning

confidence: 95%

“…However, they can be used also as model systems in the research focused on the effect of various substances on roots, e.g. growth hormones (Biondi et al, 1997;Taylor and van Staden, 1998;Vanhala et al, 1998;Ba´lva´nyos et al, 2001), oligosaccharides (Lozovaya et al, 1993;Zabotina et al, 1997;De Ruijter et al, 1998), heavy metals (Guadagnini et al, 1999), etc. Most of these studies were focused on root induction, elongation, and on root/root cultures biomass production.…”

Section: Introductionmentioning

confidence: 99%

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Effect of auxins on Karwinskia humboldtiana root cultures

Kollárová

Lišková

Kákoniová

et al. 2004

Plant Cell, Tissue and Organ Culture

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Effects of auxins (IAA, IBA and NAA) on K. humboldtiana root culture cultivated in 16-h photoperiod or in dark have been observed. Light affected positively the production of biomass when cultivated on medium supplemented with NAA in 10 and 25 lmol l À1 concentrations. In the presence of IAA and IBA these values were significantly lower. The growth dynamics of root cultures depended on the auxin used. The best adventitious roots elongation and lateral roots induction on media supplemented with IBA has been ascertained. Morphological and anatomical differences in dependence on auxin used were observed. NAA supported the formation of huge callus-like mass besides mostly very short roots, especially under the light. Similarly IAA induced short roots, and IBA seems to be the most effective substance for the root elongation in this model system. NAA induced roots with larger diameter under the light compared with the other two auxins used. The reason is in the different anatomical structure of roots which was characterized by higher number of cell layers and large intercellulars in the cortex. The shape of cortical cells in the presence of IBA depended on the light conditions. Isodiametric cortical cells were present in roots cultivated in 16-h photoperiod, irregularly-shaped cells in the dark. The effect of light conditions was the smallest in the case of roots grown on IAA enriched media.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Effect of auxins on Karwinskia humboldtiana root cultures

Kollárová

Lišková

Kákoniová

et al. 2004

Plant Cell, Tissue and Organ Culture

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“…Quantitative tropane-alkaloid determinations were performed on methanol extracts of the hairy-root clones and on samples of the medium using enzyme immunoassay (Vanhala et al 1998) and radio-immunoassay (Oksman-Caldentey et al 1987b) for scopolamine and hyoscyamine, respectively. The tropane-alkaloid content of the leaves of the H. muticus parent plant (late¯owering stage) used for transformation was also analyzed.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering

Jouhikainen

Lindgren

Jokelainen

et al. 1999

Planta

Self Cite

148

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In order to test the possibility of enhancing the production of pharmaceutically valuable scopolamine in transgenic cultures, the 35S-h6h transgene that codes for the enzyme hyoscyamine-6b-hydroxylase (EC 1.14.11.11) was introduced into Hyoscyamus muticus L. strain Cairo (Egyptian henbane). This plant was chosen for its capability to produce very high amounts of tropane alkaloids (up to 6% of the dry weight in the leaves of mature plant). To our knowledge, this is the ®rst time such a large population of transgenic cultures has been studied at the morphological, chemical and genetic levels. A great variation was observed in the tropane alkaloid production among the 43 positive transformants. The best clone (KB7) produced 17 mg/l scopolamine, which is over 100 times more than the control clones. However, conversion of hyoscyamine to scopolamine was still incomplete. The expression of h6h was found to be proportional to the scopolamine production, and was the main reason behind the variation in the scopolamine/hyoscyamine ratio in the hairy-root clones. These results indicate that H. muticus strain Cairo has a potential for even more enhanced scopolamine production with more ecient gene-expression systems.

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Plant Cell Culture, Secondary Product Accumulation

Zhou

Zhong

2010

Encyclopedia of Industrial Biotechnology

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Plant cell culture is an effective technology for the production of valuable plant‐derived secondary metabolites which can be used as pharmaceuticals, food additives, nutraceuticals, etc. The biosynthesis of secondary metabolites by cell culture is safe, controllable, and economical in contrast to field cultivation of their parent plants; but it is also affected by many internal and external factors. In this chapter, the background of secondary product accumulation by plant cell culture is overviewed at first. The progress in recent years toward manipulation of environmental factors, bioprocessing strategies, and elicitation and signal transduction engineering are summarized with typical examples for each case. Furthermore, advances in modulation of secondary metabolite accumulation including heterogeneity engineering, metabolic engineering, and omics study of secondary metabolite biosynthesis, are also reviewed. Further developments in rational bioprocessing and in systems biotechnology of plant cells for metabolite production are expected in the future.

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Effect of growth regulators on transformed root cultures of Hyoscyamus muticus

Cited by 43 publications

References 22 publications

Effect of auxins on Karwinskia humboldtiana root cultures

Effect of auxins on Karwinskia humboldtiana root cultures

Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering

Plant Cell Culture, Secondary Product Accumulation

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