2010
DOI: 10.4314/tjpr.v9i1.52031
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Effect of Growth Regulators and Culture Conditions on Direct Root Induction of <i>Rauwolfia serpentina</i> L. (Apocynaceae) Benth by Leaf Explants

Abstract: Purpose: Rauwolfia serpentina (L.) Benth, from the family, Apocynaceae, is an important medicinal plant due to the alkaloid content of its root. The purpose of this study was to obtain roots directly from leaf explant using growth regulators. Methods: The leaf explant was inoculated on MS (Murashige and Skoog) medium supplemented with single and combinations of growth regulators. Root growth was also observed on liquid MS medium and under dark conditions. The reserpine content of the roots obtained was determi… Show more

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Cited by 22 publications
(10 citation statements)
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“…A number of studies have shown that 2,4-D is inevitable and the choice of auxin for successful callus induction (Pandey et al, 2010;Shetty et al, 2014;Chaudhury et al, 2016). Later, it was found that addition of cytokinins is considered as the most important for enhancing the callus induction frequency.…”
Section: Induction Of Callusmentioning
confidence: 99%
See 1 more Smart Citation
“…A number of studies have shown that 2,4-D is inevitable and the choice of auxin for successful callus induction (Pandey et al, 2010;Shetty et al, 2014;Chaudhury et al, 2016). Later, it was found that addition of cytokinins is considered as the most important for enhancing the callus induction frequency.…”
Section: Induction Of Callusmentioning
confidence: 99%
“…This is consistent with the report of Pandey et al (2010) who have reported higher frequency of callus formation when MS media was fortified with BAP and NAA. The above authors have also added that NAA as being the best plant growth regulator for producing green, compact and fast growing R. serpentina callus (Pandey et al, 2010). Rashmi and Trivedi (2016) studied the effects of different combinations and concentrations of plant growth regulators on callus growth and shown maximum callusing response (75% in stem and 77% in leaf) when MS media was supplemented with a combination of BAP (0.5 mg/L -1 ) and NAA (1 mg/L -1 ).…”
Section: Induction Of Callusmentioning
confidence: 99%
“…The cells were harvested at different periods (2,6,12,24, and 48 h, respectively). In the second set, 12-day-old cultures were treated with catharanthine in five final concentrations (1,5,10,15, 20 mg.l -1 ). The cultures were harvested at 12 h. Controls treated with the same volume of DMSO.…”
Section: Treatment With Catharanthinementioning
confidence: 99%
“…There were still about 121 chemical substances identified from different medicinal plant which are used throughout the world. 1 Rauwolfia serpentina is perceived as Rauwolfia in Hindi, Hindustani Snake Root in English, Amalpori in Malayalam, Chandra in Bengali, and so forth. It is a sage of restorative worth marked in ayurvedic, western arrangement of prescription.…”
Section: Introductionmentioning
confidence: 99%