Effect of Growth Regulators and Culture Conditions on Direct Root Induction of <i>Rauwolfia serpentina</i> L. (Apocynaceae) Benth by Leaf Explants

Abstract

Purpose: Rauwolfia serpentina (L.) Benth, from the family, Apocynaceae, is an important medicinal plant due to the alkaloid content of its root. The purpose of this study was to obtain roots directly from leaf explant using growth regulators. Methods: The leaf explant was inoculated on MS (Murashige and Skoog) medium supplemented with single and combinations of growth regulators. Root growth was also observed on liquid MS medium and under dark conditions. The reserpine content of the roots obtained was determined by HPLC.Results: Two combinations of auxins namely, para-amino benzoic acid (PABA) + α-naphthalene acetic acid ( NAA), and 3-Indole butyric acid (IBA) + NAA, promoted better root growth compared to single auxin treatment. The highest number of roots and regeneration response was observed on leaf explant cultured on MS media supplemented with PABA (1 mg l -1 ) + NAA (4 mg l -1 ). Liquid MS media gave slower growth, reduced number of roots, shorter root length as well as absence of reserpine, using the same combination of growth regulators, compared to solid MS media. The culture incubated under dark conditions produced thin roots. HPLC analysis of the regenerated roots indicated low alkaloid (reserpine) content (0.01 -0.03). However, higher alkaloid content (0.03%) was observed in cultures with fewer numbers of roots. Conclusion: A simple and reliable protocol for direct induction of roots from leaf explant of R. serpentina using plant growth regulators has been developed.

Evaluation of α-amylase expression and analysis of phytochemical in the leaf callus tissue of Rauvolfia serpentina (Linn.) Benth. Ex Kurz exposed to Cyclodextrin

“…A number of studies have shown that 2,4-D is inevitable and the choice of auxin for successful callus induction (Pandey et al, 2010;Shetty et al, 2014;Chaudhury et al, 2016). Later, it was found that addition of cytokinins is considered as the most important for enhancing the callus induction frequency.…”

“…This is consistent with the report of Pandey et al (2010) who have reported higher frequency of callus formation when MS media was fortified with BAP and NAA. The above authors have also added that NAA as being the best plant growth regulator for producing green, compact and fast growing R. serpentina callus (Pandey et al, 2010). Rashmi and Trivedi (2016) studied the effects of different combinations and concentrations of plant growth regulators on callus growth and shown maximum callusing response (75% in stem and 77% in leaf) when MS media was supplemented with a combination of BAP (0.5 mg/L -1 ) and NAA (1 mg/L -1 ).…”

Eliciting Effect of Catharanthine on the Biosynthesis of Vallesiachotamine and Isovallesiachotamine in Catharanthus roseus Cambial Meristematic Cells

“…The cells were harvested at different periods (2,6,12,24, and 48 h, respectively). In the second set, 12-day-old cultures were treated with catharanthine in five final concentrations (1,5,10,15, 20 mg.l -1 ). The cultures were harvested at 12 h. Controls treated with the same volume of DMSO.…”

Antihypertensive and Antihyperlipidemic Activity of Aqueous Methanolic Extract ofRauwolfia Serpentinain Albino Rats

“…There were still about 121 chemical substances identified from different medicinal plant which are used throughout the world. 1 Rauwolfia serpentina is perceived as Rauwolfia in Hindi, Hindustani Snake Root in English, Amalpori in Malayalam, Chandra in Bengali, and so forth. It is a sage of restorative worth marked in ayurvedic, western arrangement of prescription.…”