Effect of Growth Media and Incubation Time on the Culturability of Soil Bacteria

Chikere, Chioma Blaise; Udochukwu, U.

doi:10.9790/3008-09210609

Cited by 7 publications

(3 citation statements)

References 14 publications

(17 reference statements)

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“…Then, 35 colonies were picked up based on their different colony morphology and streaked on NA plates for further purification. The media could provide nutrients for soil bacteria; this could be attributed to the high nutrient composition of the nutrient agar [12]. The basic phenotypic determination of each isolates includes morphological and biochemical characterization such as cell shape, gram staining, and catalase test.…”

Section: Resultsmentioning

confidence: 99%

Visual screening of potassium solubilizing bacteria for increasing the solubilization of potassium minerals

Akasah,

Chou

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Research on potassium solubilizing bacteria (KSB) has recently gained attention, most of it has been focused on the size of the halo zone; however, information regarding KSB growth duration and their potential to reduce the use of chemical fertilizers for sustainable agriculture and to be used as a biofertilizer are still lacking. The aim of this study was to offer a complete, long-term observation of KSB growth. We monitored the growth of two KSB strains that were isolated from the rhizosphere for 7 days on Modified Aleksandrov Medium (MAM) plates. We specifically designed the observation times. These methods were used to distinguish KSB’s capability to solubilize potassium minerals. The results indicated that KSB-12 isolate was capable of producing organic acid and thus solubilizing potassium faster than KSB-19 isolate at 1-4 days of incubation. Interestingly, the solubilizing zone of the KSB-12 isolate decrease after 5 days of incubation, while KSB-19 isolate continued to grow, maintain cell numbers, produce organic acids, and continuously release potassium. Different types of volatile and non-volatile organic acids have an effect on this phenomenon. In conclusion, we provided a well-designed time series study regarding the potassium solubilization efficiency of KSB, determining the fastest and longest strains of potential KSBs.

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Section: Resultsmentioning

confidence: 99%

Visual screening of potassium solubilizing bacteria for increasing the solubilization of potassium minerals

Akasah,

Chou

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…The growth of bacteria and fungi on culture media was optimum on the third day after being grown. This is also influenced by the incubation temperature because the temperature of the origin of bacterial growth will affect its growth in culture media (Chikere and Udochukwu, 2014).…”

Section: Resultsmentioning

confidence: 99%

Effect of Antimicrobial Plants on Indigenous Microorganisms from Rumen Bos Taurus in The Fermentation Process

Meyuliana,

Astuti

2023

JANAPS

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Indigenous microorganisms (IMO) are used in various agricultural activities. IMO used as a bioactivator for animal feed fermentation. IMO from bovine rumen B. taurus can be used as a bioactivator but it has contamination with pathogenic microorganisms. The antimicrobial plants tested to suppress the growth of pathogenic microorganisms during fermentation were Syzygium polyanthum, Cassia alata, Anredera cordifolia, and Piper betle. Antimicrobial plant fermented with rumen fluid. The results of IMO fermentation with antimicrobials were then analyzed for the microorganisms that grew in them and the display of the number of microorganisms growing. The results of the most growth of microorganisms are without antimicrobial treatment. The suppression of microbial growth on indigenous microorganism fermentation with antimicrobial plant C. alata obtained the highest yield, which was able to suppress up to 84% of microbial growth on NA medium.

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“…In this work Pseudomonas aeruginosa (MTCC 2453, 647) strains are selected for the study. Cultivation of selected bacterial strains (Pseudomonas aeruginosa MTCC2453, 647) is done in Asparagine-proline (AP) broth using enrichment method 3 . Asparagine-proline (AP) broth was enriched with copper sulphate, methylamine, of aluminum to increase the yield of azurin 4 .…”

Section: Methodsmentioning

confidence: 99%

Untitled

2018

IJPSR

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The pretentious extract of Pseudomonas aeruginosa has shown promising anticancer activity. It contains a protein called Azurin. It has a molecular weight of 14 kDa. It is soluble in water. It is a type I coppercontaining protein of cupredoxin family. In this work, Pseudomonas aeruginosa MTCC strain 647 was cultivated in modified Asparagine-proline broth. An attempt was done to isolate and purify azurin from Pseudomonas aeruginosa (MTCC 647) by using chromatography on sephadex G, CM cellulose and SDS PAGE electrophoresis. Recent studies have found that Pseudomonas aeruginosa can synthesize nano particles through either intracellular or extracellular mechanisms by bio synthetic means. Hence, the composite nano particles (Azurin-Ag NP) were synthesized by biosynthetic methods. P. aeruginosa MTCC strains 647 was cultivated in modified Asparagine-proline broth initially to produce shake flask cultures and then fed batch cultivation. From the bacterial extracted Azurin is isolated and purified to get a final concentration of 4.6 mg/g dry bacteria. As the nano particle mediated drug delivery effectively deliver the drug to the tumor cells, the composite nano particles (Azurin-Ag NP) were synthesized by biosynthetic methods. These bio synthetically produced Ag nano particles has loaded maximum 36 ng of Azurin protein. Pseudomonas aeruginosa MTCC strains 647 can be chosen as strain to produce Azurin in large scale. The anti-cancer activity of azurin is complimented with bio synthetic production of silver nano particles containing Azurin (Azurin-Ag-NPs). INTRODUCTION: Cancer treatment is done by radiation, chemotherapy, surgery and immunotherapy. Microorganisms and their products are found to prevent cancer regression with a remarkable anti-cancer activity 1 .

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Effect of Growth Media and Incubation Time on the Culturability of Soil Bacteria

Cited by 7 publications

References 14 publications

Visual screening of potassium solubilizing bacteria for increasing the solubilization of potassium minerals

Visual screening of potassium solubilizing bacteria for increasing the solubilization of potassium minerals

Effect of Antimicrobial Plants on Indigenous Microorganisms from Rumen Bos Taurus in The Fermentation Process

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