Effect of growth factors on matrix synthesis by human nasal chondrocytes cultured in monolayer and in agar

Bujía, J; Pitzke, P.; Kastenbauer, E.; Wilmes, E.; Hammer, C.

doi:10.1007/bf00178288

Cited by 28 publications

(20 citation statements)

References 25 publications

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“…Members of the TGF-β superfamily play a major role in cartilage development and repair. Mainly, the isoforms TGF-β1, 2 and 3 enhance chondrocyte proliferation and increase ECM synthesis by chondrocytes (Morales 1991; Bujia et al 1996; van der Kraan et al 1992). Further, TGF-β1 and 3 promote chondrogenesis of MSCs (Grimaud et al 2002; Li et al 2005; Schulz et al 2008; Puetzer et al 2010; Xu et al 2008).…”

Section: Important Parameters For Cartilage Tissue Engineering Studiementioning

confidence: 99%

Tissue engineering of functional articular cartilage: the current status

2011

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Osteoarthritis is a degenerative joint disease characterized by pain and disability. It involves all ages and 70% of people aged >65 have some degree of osteoarthritis. Natural cartilage repair is limited because chondrocyte density and metabolism are low and cartilage has no blood supply. The results of joint-preserving treatment protocols such as debridement, mosaicplasty, perichondrium transplantation and autologous chondrocyte implantation vary largely and the average long-term result is unsatisfactory. One reason for limited clinical success is that most treatments require new cartilage to be formed at the site of a defect. However, the mechanical conditions at such sites are unfavorable for repair of the original damaged cartilage. Therefore, it is unlikely that healthy cartilage would form at these locations. The most promising method to circumvent this problem is to engineer mechanically stable cartilage ex vivo and to implant that into the damaged tissue area. This review outlines the issues related to the composition and functionality of tissue-engineered cartilage. In particular, the focus will be on the parameters cell source, signaling molecules, scaffolds and mechanical stimulation. In addition, the current status of tissue engineering of cartilage will be discussed, with the focus on extracellular matrix content, structure and its functionality.

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Section: Important Parameters For Cartilage Tissue Engineering Studiementioning

confidence: 99%

Tissue engineering of functional articular cartilage: the current status

2011

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“…[11][12][13][14] Members of the TGF-b superfamily play a major role in cartilage development and repair. [15][16][17] Last but not least, insulinlike growth factor 1 can stimulate the anabolic activity of chondrocytes and can induce chondrogenesis of mesenchymal stem cells. [18][19][20][21][22] In dentistry, PRGF-Endoret is already very much used (whether for uses for implantology, periodontal, or oral surgery) because it guarantees a better clinical recovery not only with a reduction of the postsurgery swelling and pain but also with a better osseointegration of the fixtures or in bone regeneration procedures.…”

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confidence: 99%

Temporomandibular Joint Disorders Treated With Articular Injection

Giacomello

Giacomello²,

Mortellaro³

et al. 2015

Journal of Craniofacial Surgery

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The objective of this study was to evaluate the effectiveness of the temporomandibular joint (TMJ) osteoarthritis treatment through articular injections of plasma rich in growth factors (PGRF)-Endoret. Thirteen patients (median age, 47.64 y; SD, 7.51; range, 40-64 y; male-female ratio, 2:11) with osteoarthritis of TMJ associated to chronic pain have been selected. They were treated with articular injections of PRGF-Endoret, measuring the maximum mouth opening and pain level before the first injection (t0), 30 days after just before the second (t1), and after 6 months (t2). Data were analyzed using the paired Student's t-test data. The visual analogue scale score at t0 is 7.69 (range, 4-10; SD, 1.9), whereas that at t1 is 1.54 (range, 0-5; SD, 1.74) and that at t2 is 0.23 (range, 0-2; SD, 0.65). These differences in the results are statistically highly significant (P < 0.0001 comparison t0-t1 and t0-t2 and P < 0.01 comparison t1-t2). In terms of maximum mouth opening, it reduced from 30.15 mm at t0 (range, 26-40 mm; SD, 4.44) to 37.54 mm at t1 (range, 31-51 mm; SD, 5.10), with an increase of 7.38 mm (range, 4-11 mm; SD, 2.02) and a highly significant difference (P < 0.0001). At t2, it was 39.54 mm (range, 34-51; SD, 4.55) with an increase of 9.38 mm (range, 5-12 mm; SD, 2.21) compared with t0 and that of 2.00 mm compared with t1. Both differences in the results are statistically significant (P < 0.0001 and P < 0.01, respectively). The articular injections of PRGF-Endoret represent a very efficient method to control pain and to improve the TMJ mobility.

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“…Insulin-like growth factor-I (IGF-I), transforming growth factor b (TGFb)-1, TGFb-2, bone morphogenetic protein (BMP)-2, and BMP-7 have all been shown to improve chondrogenesis during 3D culture either in serum-free medium or in medium supplemented with fetal bovine serum (FBS). [6][7][8] We recently reported that culture of human nasal septal chondrocytes with pooled human serum (HS) results in increased proliferation and chondrogenesis compared with FBS. 9 The goal of this study was to identify growth factor combinations that improve chondrogenesis during 3D culture of human nasal septal chondrocytes in the medium supplemented with HS.…”

mentioning

confidence: 99%

Insulin-like Growth Factor-I and Growth Differentiation Factor-5 Promote the Formation of Tissue-Engineered Human Nasal Septal Cartilage

Alexander

Sage²,

Chen

et al. 2010

Tissue Engineering Part C: Methods

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Introduction: Tissue engineering of human nasal septal chondrocytes offers the potential to create large quantities of autologous material for use in reconstructive surgery of the head and neck. Culture with recombinant human growth factors may improve the biochemical and biomechanical properties of engineered tissue. The objectives of this study were to (1) perform a high-throughput screen to assess multiple combinations of growth factors and (2) perform more detailed testing of candidates identified in part I. Methods: In part I, human nasal septal chondrocytes from three donors were expanded in monolayer with pooled human serum (HS). Cells were then embedded in alginate beads for 2 weeks of culture in medium supplemented with 2% or 10% HS and 1 of 90 different growth factor combinations. Combinations of insulin-like growth factor-I (IGF-1), bone morphogenetic protein (BMP)-2, BMP-7, BMP-13, growth differentiation factor-5 (GDF-5), transforming growth factor b (TGFb)-2, insulin, and dexamethasone were evaluated. Glycosaminoglycan (GAG) accumulation was measured. A combination of IGF-1 and GDF-5 was selected for further testing based on the results of part I. Chondrocytes from four donors underwent expansion followed by threedimensional alginate culture for 2 weeks in medium supplemented with 2% or 10% HS with or without IGF-1 and GDF-5. Chondrocytes and their associated matrix were then recovered and cultured for 4 weeks in 12 mm transwells in medium supplemented with 2% or 10% HS with or without IGF-1 and GDF-5 (the same medium used for alginate culture). Biochemical and biomechanical properties of the neocartilage were measured. Results: In part I, GAG accumulation was highest for growth factor combinations including both IGF-1 and GDF-5. In part II, the addition of IGF-1 and GDF-5 to 2% HS resulted in a 12-fold increase in construct thickness compared with 2% HS alone ( p < 0.0001). GAG and type II collagen accumulation was significantly higher with IGF-1 and GDF-5. Confined compression modulus was greatest with 2% HS, IGF-1, and GDF-5. Conclusion: Supplementation of medium with IGF-1 and GDF-5 during creation of neocartilage constructs results in increased accumulation of GAG and type II collagen and improved biomechanical properties compared with constructs created without the growth factors.

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Effect of growth factors on matrix synthesis by human nasal chondrocytes cultured in monolayer and in agar

Cited by 28 publications

References 25 publications

Tissue engineering of functional articular cartilage: the current status

Tissue engineering of functional articular cartilage: the current status

Temporomandibular Joint Disorders Treated With Articular Injection

Insulin-like Growth Factor-I and Growth Differentiation Factor-5 Promote the Formation of Tissue-Engineered Human Nasal Septal Cartilage

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