Effect of growth factors on the activation of human Tenon’s capsule fibroblasts

Denk, Peter Oliver; Hoppe, Jürgen; Hoppe, Viviane; Knorr, M.

doi:10.1076/ceyr.27.2.35.15456

Cited by 35 publications

(27 citation statements)

References 21 publications

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“…Tenon’s capsule fibroblasts exposed to MMC have been reported to exhibit a significant decrease in the number of FGF-β receptors 40–44. In our study, MMC and infliximab equally decreased the immunostaining intensity of FGF-β in the bleb region.…”

Section: Discussionsupporting

confidence: 65%

“…On the other hand, elevated levels of TGF-β are associated with increased fibrosis in several organs, including the heart, liver, kidney, lung, skin, and bone marrow 39–42. TGF-β is considered to inhibit the growth of leukocytes and to stimulate the production of fibroblasts 43,44. TGF-β enhances resistance of the wound site to tension in corneal incisions in rabbits by enhancing the number of fibroblasts through chemotaxis, reducing the synthesis of matrix metalloprotease, and enhancing the synthesis of metalloprotease tissue inhibitor 42.…”

Section: Discussionmentioning

confidence: 99%

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Topical infliximab for the suppression of wound healing following experimental glaucoma filtration surgery

Turgut¹,

Eren²,

Akın³

et al. 2014

DDDT

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BackgroundThe purpose of this work was to look into the effects of infliximab on wound healing in experimental glaucoma filtration surgery and to compare the antifibrotic effects of this agent to that of mitomycin-C (MMC).MethodsTwenty-eight male New Zealand White rabbits were randomly assigned to four groups, each including seven rabbits: control group, sham group, MMC group, and infliximab group. The rabbits in the control group were not operated on and did not receive any treatment. The rabbits in the sham group underwent trabeculectomy and had one drop of saline instilled four times a day for 14 days. The rabbits in the MMC treatment group underwent trabeculectomy, and a sponge soaked in 0.4 mg/mL MMC was applied intraoperatively to the scleral surgical site for three minutes. The rabbits in the infliximab treatment group underwent trabeculectomy and one drop of 10 mg/mL infliximab was instilled four times a day for 14 days after surgery. On day 14 of the experiment, the operated and control eyes were enucleated and histologically and immunohistochemically analyzed.ResultsThe mean fibroblast and mononuclear cell (MNC) numbers and the mean immunostaining intensities of transforming growth factor-β (TGF-β), fibroblast growth factor-β (FGF-β), and platelet-derived growth factor (PDGF) in the sham group were higher than those of the control group (P<0.01). The mean fibroblast and MNC numbers and the mean immunostaining intensities of TGF-β, FGF-β, and PDGF in the MMC and infliximab groups were statistically significantly lower than those of the sham group (P<0.01). The mean fibroblast and MNC numbers and the mean TGF-β, FGF-β, and PDGF immunostaining intensities of the MMC and infliximab groups were similar (P>0.05).ConclusionOur study suggests that topical infliximab effectively suppresses the subconjunctival wound healing response after experimental glaucoma filtration surgery, reducing the MNC and fibroblast numbers and immunostaining intensities of TGF-β, FGF-β, and PDGF.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Topical infliximab for the suppression of wound healing following experimental glaucoma filtration surgery

Turgut¹,

Eren²,

Akın³

et al. 2014

DDDT

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“…Degree and kinetics of proliferation differed, however, between media conditioned by the three cancer cell lines which is in line with the known fact that induction of fibroblastic stroma can differ between different tumours. We also identified in prostate cancer cell conditioned media growth factors (such as bFGF and PDGF) to which induction of fibroblast proliferation can be attributed (61,62). Basic fibroblast growth factor is already known to induce stromal growth within the human prostate (63).…”

Section: Discussionmentioning

confidence: 91%

Tumour-stroma interactions between metastatic prostate cancer cells and fibroblasts

Kaminski

Hahne²,

Haddouti³

et al. 2006

Int J Mol Med

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Abstract.Previous work has shown the importance of tumourstroma interactions for prostate cancer development at the primary site. The aim of the present study was to find out whether evidence can be found for a tumour-stroma crosstalk also between metastatic prostate cancer cell lines and non-prostatic stromal fibroblasts which are encountered by metastatic cells at most sites. We addressed this issue in cell culture systems using 3 metastatic human prostate cancer cell lines (LnCaP, PC-3 and DU-145) on the one hand, and a human fibroblast line (HFF, human foreskin fibroblasts) on the other. We incubated fibroblasts with tumour cell-and tumour cells with fibroblast-conditioned media and evaluated several parameters important for the establishment of metastases such as cell proliferation, migration and expression of matrix degrading proteases. We also determined in the conditioned media the concentrations of several growth factors and cytokines which might be responsible for the observed effects. We found that media conditioned by all 3 metastatic prostate cancer cell lines stimulated fibroblast proliferation which corresponds to fibrous stroma induction in vivo. DU-145 cell conditioned media induced in fibroblasts expression of mmp-1 mRNA known to be important for tumour invasion. ELISA assays revealed that tumour cells secrete bFGF, PDGF and TNF· known to stimulate fibroblast proliferation and/or MMP-1 expression. Cultivation of DU-145 carcinoma cells in fibroblast conditioned medium resulted in an enhanced proliferation and anchorage-independent growth of this cell line in soft agar. Fibroblast conditioned medium also increased migration of PC-3 cells in the wound assay and slightly augmented mmp-1 expression. KGF (able to stimulate proliferation of normal and neoplastic prostate epithelial cells) was secreted by fibroblasts at higher concentrations than by all 3 tumour cell lines. In addition, fibroblasts secreted TNF·, bFGF, PDGF, HGF and also VEGF, the most important factor for tumour vascularization. Our results provide evidence that tumour-stroma interactions do not only exist at the primary site but also between metastatic prostate cancer cell lines and their fibroblastic microenvironment. These interactions, which are mediated through secreted factors, affect several steps of the metastatic cascade including proliferation, anchorage-independent growth, migration and the secretion of matrix-degrading proteases.

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“…Several studies reported that these factors are potent stimulators of HTF proliferation in vitro (Cunliffe et al, 1995;Denk et al, 2003;Knorr et al, 1997). Furthermore, inhibition of some of these molecules diminished growth of cultured human TF (Salas-Prato et al, 1996) and improved glaucoma filtration surgery outcome in an experimental glaucoma model (Turgut et al, 2014).…”

Section: Other Growth Factors and Cytokinesmentioning

confidence: 99%