Effect of growth factors, EGF and IGF-I, and estradiol on in vitro maturation of sheep oocytes

Güler, Ayşe; Poulin, Noëlle; Mermillod, Pascal; Terqui, M.; Cognie, Y.

doi:10.1016/s0093-691x(00)00342-3

Cited by 127 publications

(86 citation statements)

References 26 publications

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“…For example, Shabankareh et al [123] obtained good results using human menopausal serum and Birler et al [124] showed that, in sheep, SOF medium improves the rate of cleavage compared to TCM199 medium. Also, Guler et al [125] obtained better results adding follicular fluid to maturation medium or using epidermal growth factor (EGF) conjugated with FSH and E 2 [50]. The positive effect of EGF or other EGF-like ligands in COCs has been shown during IVM [126,127], whereas other researchers [101,128] preferred EGF and cysteamine.…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservation are unattractive for commercial purposes. It is expected that a successful cryopreservation of sheep embryos can push forward all other reproductive biotechnologies in this species, such as multiple ovulation and embryo transfer (MOET), artificial insemination, or in vitro production of embryos. This paper tries to discuss the current and future perspectives of cryopreservation of in vivo-and in vitro-produced sheep embryos concerning advantages and limitations for its practical use and possible solutions for improving methods to allow a higher survival rate of cryopreserved embryos.

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Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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“…Insulin and IGF are present at the 8-cell stage murine embryo and have also been detected in the reproductive tract (Henemyre and Markoff 1999;Heyner et al 1989). Insulin and IGF improve in vitro maturation outcomes in a variety of species and the addition of insulin and IGF ligands to culture media increases cell proliferation, blastocyst development rates and increases ICM cell numbers in the mouse (Gardner and Kaye 1991;Guler et al 2000;Harvey and Kaye 1992;Kaye and Gardner 1999;Makarevich and Markkula 2002). In addition the presence of IGF can also protect against the negative effects of oxidative stress (Kurzawa et al 2002).…”

Section: Growth Factorsmentioning

confidence: 99%

The Future of Human Embryo Culture Media – Or Have We Reached the Ceiling?

Zander-Fox¹,

Lane²

2012

The Human Embryo

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“…Recently, Shirazi et al (2010) reported a positive effect of GH added in vitro to mature ovine oocyte that resulted in 73% of hatched blastocyst development. Insulin-like growth factor-I (IGF-I) has also been known for stimulating oocyte maturation and promoting blastocyst development in several species, such as rabbits (Yoshimura et al, 1994), bovine (Matsui et al, 1995) and sheep (Guler et al, 2000); therefore, those findings demonstrated that IGF-I is critical for embryo culture. In horses, IGF-I is known to promote nuclear and cytoplasm maturation of in vitro matured equine oocytes (Carneiro et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Influence of equine growth hormone, insulin-like growth factor-I and its interaction with gonadotropins on in vitro maturation and cytoskeleton morphology in equine oocytes

Pereira

Lorenzo

Carneiro

et al. 2013

Animal

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In horses, successful in vitro fertilization procedures are limited by our inability to consistently mature equine oocytes by in vitro methods. Growth hormone (GH) is an important regulator of female reproduction in mammals, playing an important role in ovarian function, follicular growth and steroidogenesis. The objectives of this research were to investigate: the effects of equine growth hormone (eGH) and insulin-like growth factor-I (IGF-I) on the in vitro maturation (IVM) of equine oocytes, and the effects of eGH in addition to estradiol (E 2 ), gonadotropins (FSH and LH) and fetal calf serum (FCS) on IVM. We also evaluated the cytoskeleton organization of equine oocytes after IVM with eGH. Equine oocytes were aspirated from follicles ,30 mm in diameter and matured for 30 h at 38.58C in air with 5% CO 2 . In experiment 1, selected cumulus-oocyte complexes (COCs) were randomly allocated as follows: (a) control (no additives); (b) 400 ng/ml eGH; (c) 200 ng/ml IGF-I; (d) eGH 1 IGF-I; and (e) eGH 1 IGF-I 1 200 ng/ml anti-IGF-I. In addition to these treatment groups, we also added 1 mg/ml E 2 , 5 IU/ml FSH, 10 IU/ml LH and 10% FCS in vitro (experiment 2). Oocytes were stained with markers for microtubules (anti-a-tubulin antibody), microfilaments (AlexaFluor 488 Phalloidin) and chromatin (TO-PRO 3 -iodide) and assessed via confocal microscopy. No difference was observed when eGH and IGF-I was added into our IVM system. However, following incubation with eGH alone (40%) and eGH, E 2 , gonadotropins and FCS (36.6%) oocytes were classified as mature v. 17.6% of oocytes in the control group (P , 0.05). Matured equine oocytes showed that a thin network of filaments concentrated within the oocyte cortex and microtubules at the metaphase spindle showed a symmetrical barrel-shaped structure, with chromosomes aligned along its midline. We conclude that the use of E 2 , gonadotropins and FCS in the presence of eGH increases the number of oocytes reaching oocyte competence.

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Effect of growth factors, EGF and IGF-I, and estradiol on in vitro maturation of sheep oocytes

Cited by 127 publications

References 26 publications

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

The Future of Human Embryo Culture Media – Or Have We Reached the Ceiling?

Influence of equine growth hormone, insulin-like growth factor-I and its interaction with gonadotropins on in vitro maturation and cytoskeleton morphology in equine oocytes

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