Effect of growth conditions on endo- and exopolymer biosynthesis in Anabaena cylindrica 10 C

Lama, Licia; Nicolaus, Barbara; Calandrelli, Valeria; Manca, Maria; Romano, Ida; Gambacorta, Agata

doi:10.1016/0031-9422(95)00985-x

Cited by 83 publications

(51 citation statements)

References 21 publications

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“…Cyanothece 16 SOM 2 increases EPS release under P-starvation (De Philippis et al 1993 ), while in the case of Phormidium J-1 and Cyanospira capsulata no signifi cant effects were observed (De Philippis et al 1991 ;Fattom and Shilo 1984 ). For Anabaena cylindrica 10 °C, a decrease in EPS release was observed under P-shortage (Lama et al 1996 ).…”

Section: Phosphorus Availabilitymentioning

confidence: 46%

“…Higher EPS production and cell growth in the presence of combined nitrogen source was observed for different Anabaena strains (Lama et al 1996 ). The quantity and the composition of produced polymer can also vary according to the provided N-source (De Philippis and Vincenzini 1998 ), although some exceptions can be found (Tischer and Davis 1971 ).…”

Section: Nitrogen Availabilitymentioning

confidence: 95%

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Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Rossi

Philippis

2016

The Physiology of Microalgae

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Aims and ScopeApplied Phycology, the practical use of algae, encompasses a diverse range of fields including algal culture and seaweed farming, the use of algae to produce commercial products such as hydrocolloids, carotenoids and pharmaceuticals, algae as biofertilizers and soil conditioners, the application of algae in wastewater treatment, renewable energy production, algae as environmental indicators, environmental bioremediation and the management of algal blooms. The commercial production of seaweeds and microalgae and products derived there from is a large and well established industry and new algal species, products and processes are being continuously developed.The aim of this book series, Developments in Applied Phycology, is to present state-ofthe-art syntheses of research and development in the field. Volumes of the series will consist of reference books, subject-specific monographs, peer reviewed contributions from conferences, comprehensive evaluations of large-scale projects, and other booklength contributions to the science and practice of applied phycology.

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Section: Phosphorus Availabilitymentioning

confidence: 46%

Section: Nitrogen Availabilitymentioning

confidence: 95%

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Rossi

Philippis

2016

The Physiology of Microalgae

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“…9,10,11,12,13,15,16,17,18,19,20,21,48,49,50 The active membrane transport species is almost always hydrogen-carbonate (HCO 3 -). Cyanobacteria fix carbon via the Calvin Cycle (also known as reductive pentose phosphate cycle or Calvin-Benson-Bassham cycle) driven by the energy gained from photosynthesis.…”

Section: Carbon Sourcementioning

confidence: 99%

Photo-autotrophic Production of Poly(hydroxyalkanoates) in Cyanobacteria

Drosg¹,

Fritz²,

Gattermayr³

et al. 2015

Chem.Biochem.Eng.Q.

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In the last two decades, poly(hydroxyalkanoates) (PHA) were solely produced using heterotrophic bacteria in aerobic cultivation. With respect to the great potential (500 Mt yr -1 ) of raw industrial CO 2 streams and even greater potential of flue gases, the focus on photo-autotrophic biotechnological processes is increasing steadily. Primarily, PHA-gene transfer from heterotrophic bacteria into algae and plant cells was attempted, with the intention to combine the known biosynthesis pathway with autotrophic cultivation. The natural occurrence of PHA in cyanobacteria is known at least since 1966. However, cyanobacteria were never considered for commercial production because the PHA amount based on cell mass and based on volumetric productivity is generally very low. Therefore, strain improvements were suggested, either by gene amplification or by suppression of biochemical pathways competing for the cell's acetate pool. In the late 1990s, the success of genetic modification was confirmed experimentally, elevating the cyanobacteria cell's PHA content. With additional optimization, PHB amounts up to 50 % w/w of biomass dry matter or up to about 2.4 g L -1 bioreactor volume could be produced within 11 days. Considering the land use for agriculture and the competition for plant biomass between food, feed, fuel and energy production, the binding of CO 2 in a biotechnological process using photo-autotrophic microorganisms may become a promising option.

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“…Although EPS were not analyzed during this study, formation of EPS is expected for this strain because of its mucous-colony shape, observed previously (19). The relationship between EPS production and intracellular poly(3HB) accumulation/mobilization has been studied before during balanced and unbalanced growth of many mesophilic bacterial species (9,27,45). Interestingly, the degradation of poly(3HB) can be investigated for the in vivo production of enantiomerically pure (R)-3-hydroxybutyric acid, which has various industrial and medical applications (30,47).…”

mentioning

confidence: 99%

High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10

Ibrahim

Steinbüchel

2010

Appl Environ Microbiol

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Fermentations, which are performed with thermophilic microorganisms, are energy-efficient processes because few cooling efforts are necessary. Moreover, thermophilic fermentations are self-heated systems because the heat generated by the cell's metabolism during high-cell-density (HCD) growth (28, 49) and also by stirring can be used for providing heat to the fermentation process itself. Thus, both heating and cooling costs are lowered (14, 46). Additionally, unlike when mesophilic bacteria are used in fermentations, sterile conditions may not be essential during a process involving thermophilic bacteria (41, 42).Unfortunately, thermophiles usually grow to only low cell densities. Recently, with the use of advanced fermentation technology, a few thermophilic bacteria were cultivated successfully, yielding HCD growth and enhanced product formation (15,25). In industry, HCD cultivation is often a prerequisite for high productivity during fermentations, in particular if intracellular compounds are being produced. HCD cultivations are enhanced cultivations of the microbial strain achieving cell dry weight (CDW) concentrations exceeding 100 g/liter. However, a lower cell density can be regarded as an HCD as well, depending on the microorganism and its cultivation conditions (39). In general, HCD cultivations represent a 10-to 20-fold increase in growth in comparison to normal cell density growth. Problems encountered by HCD cultivation are numerous, such as partial O 2 pressure (pO 2 ) deficiency, byproduct formation, and/or metabolic heat production. As a result of the growing industrial interest in HCD, many attempts have been made to develop HCD fermentations, for example, by improving potent strains and/or using different types of bioreactors and cultivation strategies (39).The most industrially applicable technique is fed-batch fer-

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Effect of growth conditions on endo- and exopolymer biosynthesis in Anabaena cylindrica 10 C

Cited by 83 publications

References 21 publications

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

Photo-autotrophic Production of Poly(hydroxyalkanoates) in Cyanobacteria

High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10

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