Effect of Green Tea, Stevia Extract Solutions, and Fluoride-Based Mouthwash on Remineralization of Incipient Enamel Lesion: An In Vitro Study.

Abstract

Background: Remineralization of early enamel lesions is considered one of the approaches of minimal invasive dentistry. Aim: This in vitro study was conducted to evaluate the remineralizing effect of green tea, stevia extract, and fluoride-based mouthwashes on incipient enamel lesions using a laser fluorescence device (Diagnodent). Materials and Methods: Sixty human extracted premolars were randomly distributed into six groups of 10 teeth according to the remineralizing agent used. Group I: 0.5% stevia aqueous solution; Group II: 5% stevia aqueous solution; Group III: 0.5% green tea aqueous solution; Group IV: 5% green tea aqueous solution; Group V: Fluoride mouthwash (positive control group) and Group VI: artificial saliva (negative control group). Laser fluorescence testing (Diagnodent) was used for baseline assessment, after 48 hours, demineralization, and after 1 week of the remineralization phase.Results: After 7 days of remineralization, the negative control group showed the highest laser fluorescence mean value while all experimental and positive control groups showed the lowest laser fluorescence mean values. 5% stevia and 0.05 % Green tea mouthwashes showed an insignificant difference between baseline and remineralization. Conclusions: Stevia and green tea mouthwashes had a positive impact on incipient enamel remineralization.

Remineralization and Antibacterial efficacy of Ashwagandha, Maca and Ginger-based mouthwashes versus Fluoride-based mouthwash on initial enamel caries: An in-vitro study

“…A one-way analysis of variance (ANOVA) or a comparable non-parametric test will be employed to assess the impact of various treatments and con-trols on the remineralization of early enamel caries. According to a previous study by Rohym et al (8), values after remineralization varied between (6.15±3.51), (7.78±4.58), (7.28±6.49), (11.75±1.25) and (8.82±5.54). Using G power statistical power Analysis program (version 3.1.9.4) for sample size determination (9) , A total sample size (n=50; subdivided to 10 in each group) will be sufficient to detect a large effect size (f) = 0.54, with an actual power (1-β error) of 0.8 (80%) and a significance level (α error) 0.05 (5%) for two-sided hypothesis test.…”

“…Tooth caries are initially indicated by an opaque lesion that appears milky white in opacity due to porosity in the enamel under the surface. Early stages of tooth decay can be stopped and reversed with effective plaque management and remineralization treatment (8) . Hence, there is a significant demand for natural therapeutic drugs that provide remineralizing and antimicrobial properties while minimizing adverse effects.…”

White Tea: A Review on Composition Characteristics, Extraction Techniques, and Application Potentials

“…The widely reported remineralization functionality of WTE also contributes to its effect in fighting caries. WTE treatment increased the microhardness value by respective 36% and 62% for demineralized dentin and enamel, indicating its remineralization functionality through cross-linking of the collagen network Roushdy and El-Sayed, 2023). Both the antioxidant and anti-collagenolytic activities of WTE were likely responsible for this functionality because it exhibited strong anti-elastase and anti-collagenase capacity (Daokar et al, 2020).…”

Remineralizing Effect of White Tea, Green Tea and Casein Phosphopeptide Amorphous Calcium Phosphate (CPP-ACP) on Artificially Demineralized Enamel (In-Vitro Study)

“…All roots were removed, each tooth was The samples were rinsed thoroughly with distilled water and stored in artificial saliva and evaluated again for surface microhardness (5).…”

“…Green tea contains minerals as potassium, phosphorus, calcium, and magnesium. Green tea is characterized by high fluoride content (over 1500 ppm) as well as its catechins content like epigallocatechin gallate (EGCG) which act as an enamel matrix metalloproteinases (MMPs) inhibitor (5).…”