Effect of gonadotropins on oocyte maturation in vitro: an animal model

Sha, Wei; Xu, Baozeng; Li, Mo; Liu, Di; Feng, Huai L.; Sun, Qing‐Yuan

doi:10.1016/j.fertnstert.2009.03.003

Cited by 29 publications

(23 citation statements)

References 71 publications

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“…Follicle-stimulating hormone (FSH) maintained associations between growing oocytes and cumulus cells during in vitro growth culture, probably by supporting the oocytes (Cayo-Colca et al, 2011). The FSH:Luteinizing hormone (LH) ratio in IVM culture media is directly correlated with oocyte cytoplasmic maturation (Sha et al, 2010). Schoevers et al (2003), using porcine oocytes, stated that FSH initially retards germinal vesicle breakdown, but overall has a stimulatory effect on nuclear and cytoplasmic maturation.…”

Section: Resultsmentioning

confidence: 99%

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

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-This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 -100% FF) slowed meiotic progression and CG migration (control -78.13% vs. treated -52.58% and control -52.7% vs. treated -11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control -90.13% vs. treated -82.64% and control -35.73% vs. treated -11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control -29.91 vs. treated -35.49, at 24 h of maturation) and total cell numbers (control -109.53 vs. treated -120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.

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Section: Resultsmentioning

confidence: 99%

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

et al. 2014

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“…The pattern of mitochondrial distribution in human oocytes was defined as peripheral (located largely in the cortical region of the oocytes), diffuse (spread evenly all over the cytoplasm) and semi-peripheral (between peripheral and diffused distribution) [19,20] . In most of the matured oocytes observed in the in vivo-MII group, mitochondrial distribution was diffuse ( fig.…”

Section: Mitochondrial Distributionmentioning

confidence: 99%

“…The redistribution of mitochondria is an important event in oocyte cytoplasmic maturation and for its developmental competence. Cortical granule (CG) is another important organelle in oocyte cytoplasm and its migration is linked to oocyte maturation [20] . Both mitochondrial distribution and CG migration are considered as markers of oocyte cytoplasmic maturity [19,20] .…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Changes and Methylation of Human Oocytes Vitrified at the Germinal Vesicle Stage and Matured in vitro after Thawing

Liu

Zhou

Chu

et al. 2016

Gynecol Obstet Invest

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Aim: The study aimed to assess the effect of in vitro vitrification and maturation on the nuclear configuration, cytoplasmic maturation and global DNA methylation pattern of human germinal vesicle (GV) stage oocytes. Methods: Human oocytes from infertile women were randomly assigned to one of 3 groups: (i) metaphase II (MII) oocytes matured in vivo (vivo-MII, n = 56) as controls; (ii) MII oocytes matured in vitro (vitro-MII, n = 106); and (iii) MII oocytes that were vitrified at the GV stage, warmed and matured in vitro (cryo-MII, n = 122). All MII oocytes were fixed and immunofluorescence staining for spindle, chromosome, mitochondrion and cortical granules (CGs) were performed; examination was done using immunofluorescence laser scanning confocal microscope. The expression of 5-methycytosine in these MII oocytes was also assessed. Results: No significant difference was observed between vitro-MII and cryo-MII groups with respect to oocyte maturation rate (72.4 vs. 78.3%). No significant differences were observed in the distribution of mitochondria, migration of CG and global DNA methylation pattern among the 3 study groups. However, the abnormal configuration of spindle and chromosome was significantly higher in cryo-MII group (78.9 and 84.2%) as compared to that in the vitro-MII (45.0 and 50.0%, p < 0.05) and vivo-MII groups (27.3 and 36.4%, p < 0.05). Conclusion: GV oocytes appeared to resist vitrification and retain their potential for maturation to MII stage oocytes after thawing. However, GV oocytes vitrification combined with in vitro maturation could affect the organization of spindle and chromosome.

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“…The main functions triggered by FSH in the mammalian ovary are cell proliferation, apoptosis prevention, estradiol production, cell secretion, and regulation of several other genes [5,16,38,61,69-71]. Additionally, high doses of FSH are an essential ingredient in IVM media and was shown to efficiently promote full oocyte maturation in several mammalian species in vitro including cow [30,33], mouse [72] and pig [4,73]. This in vitro FSH effect is initiated in cumulus-oocyte complex (COCs) via its receptor (FSHR) on cumulus cells (CCs).…”

Section: Introductionmentioning

confidence: 99%

FSH in vitro versus LH in vivo: similar genomic effects on the cumulus

2013

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The use of gonadotropins to trigger oocyte maturation both in vivo and in vitro has provided precious and powerful knowledge that has significantly increased our understanding of the ovarian function. Moreover, the efficacy of most assisted reproductive technologies (ART) used in both humans and livestock species relies on gonadotropin input, mainly FSH and LH. Despite the significant progress achieved and the huge impact of gonadotropins, the exact molecular pathways of the two pituitary hormones, FSH and LH, still remain poorly understood. Moreover, these pathways may not be the same when moving from the in vivo to the in vitro context. This misunderstanding of the intricate synergy between these two hormones leads to a lack of consensus about their use mainly in vitro or in ovulation induction schedules in vivo. In order to optimize their use, additional work is thus required with a special focus on comparing the in vitro versus the in vivo effects. In this context, this overview will briefly summarize the downstream gene expression pathways induced by both FSH in vitro and LH in vivo in the cumulus compartment. Based on recent microarray comparative analysis, we are reporting that in vitro FSH stimulation on cumulus cells appears to achieve at least part of the gene expression activity after in vivo LH stimulation. We are then proposing that the in vitro FSH-response of cumulus cells have similitudes with the in vivo LH-response.

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Effect of gonadotropins on oocyte maturation in vitro: an animal model

Cited by 29 publications

References 71 publications

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

Ultrastructural Changes and Methylation of Human Oocytes Vitrified at the Germinal Vesicle Stage and Matured in vitro after Thawing

FSH in vitro versus LH in vivo: similar genomic effects on the cumulus

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