Effect of gonadotrophin-releasing hormone and related analogue on human luteal cell function in vitro

Abstract

The possible direct effect of gonadotrophin-releasing hormone (GnRH) and GnRH agonist (GnRH-A; buserelin) on basal and human chorionic gonadotrophin (HCG)-stimulated progesterone (P) and cyclic AMP (cAMP) production by cultured human luteal cells was examined. Luteal cells from the early or mid-luteal phase were incubated in long-term cultures. They responded to HCG stimulation with a 2- to 3-fold increase in P production and a 2-fold increase in cAMP production. The addition of GnRH (10(-7) and 10(-5) M) or GnRH-A (10(-7) and 10(-5) M) to the medium had no effect on either basal or HCG-stimulated secretion. These results indicate that both GnRH and GnRH-A have no direct effect on human luteal steroidogenesis in vitro.

Gonadotropin releasing hormone antagonists suppress aromatase and anti-Müllerian hormone expression in human granulosa cells

“…Whereas the lower E 2 levels can be explained solely by central inhibitory effects of the GnRH-ANT, it is possible that the GnRH-ANT exerts a suppressive effect peripherally at the ovarian level through suppression of aromatase. Previous studies looking at the effect of GnRH-A on ovarian steroidogenesis in vitro and in vivo lead to conflicting results (24,(53)(54)(55)(56)(57)(58). Our data on the effect of GnRH-A on hGCs demonstrated that the GnRH-A alone as well as in combination with Bt2cAMP caused a dose-dependent stimulation of aromatase and AMH mRNA with up to 50-fold increase in aromatase expression (Fig.…”

Gonadotropin-releasing hormone antagonists do not influence the secretion of steroid hormones but affect the secretion of vascular endothelial growth factor from human granulosa luteinized cell cultures

“…Other researchers have also reported a lack of direct effects of GnRH-agonists on ovarian steroidogenesis (17,19).…”

“…Many investigators have attempted to define direct effects of GnRH-agonists on human granulosa cells (17)(18)(19)(20)(21)(22)(23)(24)(25). With respect to steroidogenesis of granulosa cells, the results reported to date are particularly contradictory.…”

Gonadotropin-Releasing Hormone in the Ovary

“…The studies on the effects of GnRH agonists on ovarian steroidogenesis have come to contradictory conclusions. Some studies have shown a lack of direct effects, [85][86][87][88] while others have suggested direct effects, reporting either inhibition or stimulation of the production of E2, P, or even both of them. [89][90][91][92][93][94][95][96] It is worth mentioning the study of Bussenot et al, 92 in which 5 different GnRH agonists were compared regarding their effect on E2 secretion for hGL cell cultures.The results showed that some GnRH agonists enhanced E2 secretion, whereas others did not.…”