Effect of Gold Nanorod Surface Chemistry on Cellular Response

Grabinski, Christin; Schaeublin, Nicole M.; Wijaya, Andy; D'Couto, Helen; Baxamusa, Salmaan H.; Hamad‐Schifferli, Kimberly; Hussain, Saber M.

doi:10.1021/nn103476x

Cited by 177 publications

(190 citation statements)

References 47 publications

Supporting

Mentioning

171

Contrasting

Unclassified

Order By: Relevance

“…GNRs stabilized with CTAB were found to cause more severe cellular responses than those coated with either thiolated polyethylene glycol 5000 or mercaptohexadecanoic acid 14. Consistent with previous findings that the cytotoxicity of nanoparticles was strongly correlated to their surface charges, the toxicity disparity stemmed from different electrostatic associations between nanoparticles and cell membranes and the membrane‐compromising effects of CTAB 9, 10, 13.…”

Section: Resultssupporting

confidence: 87%

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

et al. 2017

View full text Add to dashboard Cite

Gold nanorods (GNRs) are a unique class of metal nanostructures that have attractive potentials in biomedical applications, and the concern on their biological safety is concomitantly increasing. Hemocompatibility is extremely important as their contact with blood circulation is unavoidable during in vivo delivery. Herein, two kinds of GNRs coated with hexadecyltrimethylammonium bromide (C‐GNRs) or poly(sodium‐p‐styrenesulfonate) are used to test their potential toxicological effects in blood. C‐GNRs with positive surface charges efficiently induce hemolysis when encountering erythrocytes. Cellular internalization of C‐GNRs is found, and they subsequently bind with hemoglobin, forming bioconjugates. The interaction between hemoglobin and C‐GNR (stoichiometry 32.7:1) is regulated by electrostatic forces. Chromophores like tryptophan (Trp) are found to interact with C‐GNRs, causing enhancement in fluorescence intensity. The conformation of protein is partially altered, evidenced by decrease in α‐helical, increase in β‐sheet and random coil of hemoglobin. Although C‐GNRs do not essentially decrease oxygen binding capacity of hemoglobin, they hamper oxygen release from the protein. Heme, the oxygen binding unit, releases from hemoglobin upon C‐GNR treatment, which could contribute to C‐GNR‐induced hemolysis. This study demonstrates the hematological effects of GNRs, revealing their potential risk in biomedical applications.

show abstract

Section: Resultssupporting

confidence: 87%

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The upregulation of the multifunctional proinflammatory cytokine-encoding gene TNF-α is further indicator of oxidative stress provoked by short-term particle exposure. TNF-α upregulation has been observed as a response in HaCaT cells to 5 and 50 mg L − 1 mercaptohexadecanoic acid-stabilized Au nanorods (Grabinski et al 2011) and has been linked to toxic asbestos exposure in human epithelial cells (Driscoll et al 1997;Robledo and Mossman 1999). After 7 days of AuNP exposure upregulation of the GPx-encoding gene and downregulation of Gadd45, a physiological regulator of the apoptotic response to TNF-α (De Smaele et al 2001), as well as the upregulation of the enzyme SOD in all treated versus, all untreated samples suggest a limited, but in parts, persistent oxidative stress response.…”

Section: Discussionmentioning

confidence: 99%

Citrate gold nanoparticle exposure in the marine bivalve Ruditapes philippinarum: uptake, elimination and oxidative stress response

Volland

Hampel

Martos-Sitcha

et al. 2015

Environ Sci Pollut Res

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) are considered an important nano-sized component of the twenty-first century. Due to their unique physical and chemical properties, they are being used and developed for a wide range of promising applications in medicine, biology and chemistry. Notwithstanding their useful aspects, in recent years concern has been raised over their ability to enter cells, organelles and nuclei and provoke oxidative stress. In a laboratory-based experiment, the non-target marine bivalve Ruditapes philippinarum was used as a model organism. Uptake, elimination and molecular effects under short-term and sub-chronic exposure conditions to an environmental relevant concentration (0.75 μg L(-1)) of weakly agglomerating citrate AuNPs (∼20 nm) were studied. Our results demonstrate that at the tested concentration, the particles are readily taken up into the digestive gland > gills and can produce significant changes (p < 0.05) in oxidative stress and inflammatory response markers, as measured by phase II antioxidant enzymes and q-PCR gene expression analysis. However, the overall magnitude of responses was low, and oxidative damage was not provoked. Further, a significant elimination of Au from the digestive tract within a 7-day purification period was observed, with excretion being an important pathway. In conclusion, short-term and sub-chronic exposure to an environmental relevant concentration of citrate-stabilized AuNPs cannot be considered toxic to our model organism, while some further consideration should be given to chronic exposure effects.

show abstract

“…Here, our method will have a potential advantage over other hyperspectral techniques [19][20][21][22][23] as we can strategically select a number of wavelengths around the LSPR. This in principle allows fast readout of the LSPR of a large number of NPs, e.g.…”

Section: Hyperspectral Measurements Of Shsy-5y Cells Incubated With Hmentioning

confidence: 99%

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Fairbairn

Christofidou

Kanaras

et al. 2013

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

Hyperspectral microscopy is a versatile method for simultaneous spatial and spectroscopic characterization of nonfluorescent samples. Here we present a hyperspectral darkfield imaging system for spectral imaging of single nanoparticles over an area of 150 × 150 µm 2 and at illumination intensities compatible with live cell imaging. The capabilities of the system are demonstrated using correlated transmission electron microscopy and single-particle optical studies of colloidal hollow gold nanoparticles. The potential of the system for characterizing the interactions between nanoparticles and cells has also been demonstrated. In this case, the spectral information proves a useful improvement to standard darkfield imaging as it enables differentiation between light scattered from nanoparticles and light scattered from other sources in the cellular environment. The combination of low illumination power and fast integration times makes the system highly suitable for nanoparticle tracking and spectroscopy in live-cell experiments.

show abstract

Effect of Gold Nanorod Surface Chemistry on Cellular Response

Cited by 177 publications

References 47 publications

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

Citrate gold nanoparticle exposure in the marine bivalve Ruditapes philippinarum: uptake, elimination and oxidative stress response

Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Contact Info

Product

Resources

About