Effect of gold nanoparticles on the respiratory activity of peritoneal macrophages

Староверов, С. А.; Aksinenko, N. M.; Габалов, К. П.; Василенко, О. А.; Vidyasheva, Irina V.; Shchyogolev, S. Yu.; Дыкман, Л. А.

doi:10.1007/bf03214925

Cited by 47 publications

(25 citation statements)

References 25 publications

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“…9). This is consistent with the previous findings that AuNPs in complex with highand low-molecular-weight antigens stimulate the respiratory activity of peritoneal macrophages [48].…”

Section: Respiratory Activity Of Splenic Lymphocytessupporting

confidence: 94%

“…This finding may indicate that AuNPs conjugated to the viral antigen are able to induce T lymphocytes and NK cells to elaborate IFN-γ and trigger a cascade of reactions directed to the immunological regulation of the antiviral immune response. A similar response of the immune system to AuNPs conjugated to a viral antigen was demonstrated earlier [47,48]. IFN-γ, which is a major mediator of inflammation in viral infections, inhibits virus replication in cells, enhances the specific immune response by promoting an increased expression of major histocompatibility complex II, and activates macrophages and NK cells.…”

Section: Cytokine Production In the Sera Of Immunized Animalssupporting

confidence: 61%

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Use of a synthetic foot-and-mouth disease virus peptide conjugated to gold nanoparticles for enhancing immunological response

Дыкман

Староверов

Mezhenny³

et al. 2015

Gold Bull

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Foot-and-mouth disease is an acute, highly contagious infection of domestic and wild cloven-hoofed animals, which can be transmitted to humans. In many cases, the existing vaccines are not quite effective. The purpose of this study was to test the possibility of using gold nanoparticles as an antigen carrier and an adjuvant. The immunogenic properties of gold nanoparticles were assessed by conjugating the particles to a synthetic peptide of the VP 1 capsid protein of the foot-and-mouth disease virus. The resulting conjugate (with or without the use of complete Freund's adjuvant), a commercial vaccine, and the native peptide served to immunize guinea pigs. The titer and sensitivity of the raised antibodies were maximal for the combination comprising the nanoparticle-peptide conjugate and complete Freund's adjuvant. Antibody biosynthesis was accompanied by increased production of proinflammatory cytokines (especially IFN-γ) and by stimulation of the respiratory activity of peritoneal macrophages. The use of gold nanoparticles as a hapten carrier enhanced the immune response even when complete Freund's adjuvant was not used.

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“…9). This is consistent with the previous findings that AuNPs in complex with highand low-molecular-weight antigens stimulate the respiratory activity of peritoneal macrophages [48].…”

Section: Respiratory Activity Of Splenic Lymphocytessupporting

confidence: 94%

Section: Cytokine Production In the Sera Of Immunized Animalssupporting

confidence: 61%

Use of a synthetic foot-and-mouth disease virus peptide conjugated to gold nanoparticles for enhancing immunological response

Дыкман

Староверов

Mezhenny³

et al. 2015

Gold Bull

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“…It is known that intraperitoneal injection of GNPs increases the phagocytic activity of macrophages and lymphocytes which causes their accumulation in the reticulum cells of lymphoid tissue and determines the immunomodulatory e®ect of GNPs. 13 In other works, it was shown that GNPs may induce either pro-in°ammatory 14 or anti-in°ammatory e®ects, 15,16 depending on their size, 11 conjugation and hydrophobicity. 17 In our study, the morphological changes in the spleen were presented in the form of circulatory disorders, which were characterized by insu±cient blood supply with varying degrees of severity.…”

Section: Discussionmentioning

confidence: 98%

Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats

Bucharskaya

Pakhomy

Злобина

et al. 2016

J. Innov. Opt. Health Sci.

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At present, gold nanoparticles (GNPs) are widely used in biomedical applications such as cancer diagnostics and therapy. Accordingly, the potential toxicity hazards of these nanomaterials and human safety concerns are gaining signi¯cant attention. Here, we report the e®ects of prolonged peroral administration of GNPs with di®erent sizes (2, 15 and 50 nm) on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals. The experiment was conducted on 24 white mongrel male rats weighing 180-220 g, gold nanospheres sizes 2, 15 and 50 nm were administered orally for 15 days at a dosage of 190 g/kg of animal body weight. The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability. The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood, especially pronounced after administration of GNPs with size of 50 nm. The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow. The signs of strengthening of the processes of di®erentiation and maturation of cellular elements were found in lymph nodes, which were showed as the increasing number of immunoblasts and large lymphocytes. The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration, proliferation and di®erentiation of immune cells indicate the presence of immunostimulation e®ect of GNPs.

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“…Known, in particular, that the injection of colloidal gold may lead to its accumulation in the reticular cells of the lymphoid tissue, activation of cellular and humoral immunity of laboratory animals [9,10]. It is Interesting is that the cytotoxicity of GN has the size dependence -pronounced cytotoxicity was shown only for particles with a diameter of 1.4 nm, but not for particle diameter of 15 nm [5].…”

Section: Discussionmentioning

confidence: 99%

“…In [9,10 ] the properties of GN to deliver antigens in vivo were studied and it was found thatGN enhanced phagocytic activity of macrophages and produced lymphocytes functionalization, which probably causes the GN immunomodulating effect [10].…”

Section: Discussionmentioning

confidence: 99%

Morphokinetics of mesenterial lymphatic node cell populations at exposure of gold nanoparticles in experiment

Злобина¹,

Bugaeva²,

Maslyakova³

et al. 2012

RusOMJ

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Abstract:The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals was investigated. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological reorganizations in the mesenterial lymphatic nodes testified about activation of migration processes, the proliferation and differentiation processes of immunocompetent cells, which assumes the presence of immunomodulating action of gold nanoparticles.

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Effect of gold nanoparticles on the respiratory activity of peritoneal macrophages

Cited by 47 publications

References 25 publications

Use of a synthetic foot-and-mouth disease virus peptide conjugated to gold nanoparticles for enhancing immunological response

Use of a synthetic foot-and-mouth disease virus peptide conjugated to gold nanoparticles for enhancing immunological response

Alterations of morphology of lymphoid organs and peripheral blood indicators under the influence of gold nanoparticles in rats

Morphokinetics of mesenterial lymphatic node cell populations at exposure of gold nanoparticles in experiment

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