Effect of Goat Breeds, Semen Diluents and Freezing Methods on Sperm Freezability and Reproductive Performance

Abstract

Two experiments were designed to study the effect of semen extenders and freezing regimens on post-thaw semen motility and viability index, effect of bucks breed was also included (experiment 1). In experiment 2, semen diluents effects on reproductive performance was conducted. Semen was collected from bucks breeds (Aradi (A), Damascuss (D) and cross (½A½D). Good quality semen was divided into 4 portions, each diluted with one diluent (Milk, Na.Citrate, Tris and Na.Bicarbonate). The diluted semen was packaged into 0.5ml straws then cooled to 5°C. After equilibration, half of the packaged straws were suspended 15cm above liquid nitrogen (LN) for 15min (Freezing regimen1; slow). Other half of straws was suspended at height 15 and 5cm of LN for 10 and 5min, respectively (Freezing regimen2; rapid) before plunged into LN. Frozen semen was thawed for post-thaw motility and viability. In the second experiment, semen with good quality was extended with three types of extenders (Milk, Na.Citrate and Tris). Diluted semen were cooled to 5°C and used for AI. Results revealed that, pre-freeze semen motility was significant higher in Tris, Na.Citrate and Na.Bicarbonate than milk diluent. Post-thaw semen motility and viability were highly significant for milk and Na.Citrate than Tris and Na.Bicarbonate diluents. Post-thaw semen motility was significantly higher in Aradi and Damascus than cross breed. Post-thaw semen motility and viability revealed, significant higher means for Freezing regimen 2 than regimen 1. Milk was significantly higher than Tris and Na.Citrate diluents for fertility and fecundity. It was concluded that, regarding to post-thaw semen viability, fertility%, kidding%, fecundity% and prolificacy%, milk is preferable than Tris and Na.citrate diluents.

Effect of fumigation height and time on cryopreservation of ram semen

“…However, it was found in this study that the quality of post-thawed spermatozoa quality after fumigation at 2 cm was higher, which contrasts with the findings of previous research. This difference may be attributed to variances in spermatozoa resistance to freezing, which could be influenced by species or breeds, as well as variances in the composition of the diluent used and the pre-equilibration time 32 , 33 . Another study on human spermatozoa have shown that differences in the volume of straws can also affect semen freezing 34 .…”