Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting

Filho, Pericles Assad Hassun; Ribeiro, Mariana Antunes; Rocha, A.M.; Allegreti, José Roberto; Serafini, P.; Motta, E.L.A.

doi:10.1007/s10815-008-9263-9

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Cumulus cells are thought to play an important role in oocyte maturation and fertilization, with signaling and regulation of functions (3). Many studies have shown us that oocyte quality and fertilization potential are related to the rate of granulosa cell apoptosis in women receiving in vitro fertilization (IVF) treatment (7). It has also been shown that granulosa and cumulus cells play an important role in oocyte maturation due to cell-cell relationship, therefore the emergence of apoptosis or necrosis in granulosa cells may be associated with future embryo quality and poor IVF/ Intracytoplasmic sperm injection (ICSI) outcomes.…”

Section: Introductionmentioning

confidence: 99%

Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

İrez

Dogan

Ciraci

et al. 2020

Gynecol Obstet Reprod Med

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OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.

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Section: Introductionmentioning

confidence: 99%

Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

İrez

Dogan

Ciraci

et al. 2020

Gynecol Obstet Reprod Med

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Apoptosis of mural granulosa cells is increased in women with diminished ovarian reserve

Fan

Chang

Wei

et al. 2019

J Assist Reprod Genet

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Purpose To evaluate the relationship between apoptosis of granulosa cells in women with normal ovarian reserve versus diminished ovarian reserve, and relate that to follicular fluid hormones, and to clinical outcomes. Methods A prospective cohort study was initiated between October 2015 and June 2016 involving a total of 164 women undergoing IVF/ICSI cycles at a single IVF center. Mural and cumulus granulosa cells, and follicularfluid were collected during oocyte retrieval. Annexin V-FITC/PI apoptosis staining and flow cytometryanalysis were performed to evaluate apoptosis rate of mural granulosa cells and cumulus cells. Follicularfluid hormones were measured by ECLIA. Laboratory and clinical outcomes were analyzed. Results In mural granulosa cells, early, late and total apoptosis rates were significantly increased in women with diminished ovarian reserve when compare to women with normal ovarian reserve, along with lower AMHand progesterone levels (but higher estradiol levels) in follicular fluid. Early apoptosis rate of cumulus cellswas significantly higher in the non-pregnant group. The apoptosis rate of mural cells was negativelycorrelated with parameters related to ovarian response, oocyte yield, MII egg number, 2pn cleavagenumber, D3 good embryos number, blastocyst formation rate and frozen embryos number. A positivecorrelation was found between mural granulosa cell apoptosis and age. Conclusion A significantly higher apoptosis rate of mural granulosa cells was correlated with worse ovarian response, with fewer egg and embryo numbers in IVF/ICSI, as well as with age. Early apoptosis rate of cumulus cellsmight also have influence on clinical pregnancy. Electronic supplementary material The online version of this article (10.1007/s10815-019-01446-5) contains supplementary material, which is available to authorized users.

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GnRH agonist versus GnRH antagonist in IVF/ICSI cycles with recombinant LH supplementation: DNA fragmentation and apoptosis in granulosa cells

Lavorato

Oliveira

Petersen

et al. 2012

European Journal of Obstetrics & Gynecology and Reproductiv

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Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting

Cited by 3 publications

References 25 publications

Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?

Apoptosis of mural granulosa cells is increased in women with diminished ovarian reserve

GnRH agonist versus GnRH antagonist in IVF/ICSI cycles with recombinant LH supplementation: DNA fragmentation and apoptosis in granulosa cells

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