Effect of Gnaphalium conoideum HBK on guinea pig airway smooth muscle: role of L-type Ca2+ channels

Campos-Bedolla, Patricia; Montaño, Luis M.; Flores‐Soto, Edgar; Aguilar, Abigaı́l; Puebla, Ana María; Lozoya, Xavier; Vargas, Mario H.

doi:10.1016/j.jep.2004.11.005

Cited by 17 publications

(12 citation statements)

References 19 publications

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“…1, B and C). In these experiments, we used KCl to fully contract the tissue to demonstrate tissue viability at the end of the experiment, as described previously (46,47). Importantly, Ned-19 did not affect KCl-induced contractions (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…For these experiments, we employed cumulative concentration-response curves as commonly reported for such preparations (46,47,54). As reported (46,47), increases in carbachol concentration led to increasing force of contraction until saturation of the response (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

“…Tracheal Contractions Measured by Wire Myography-Experiments were performed as described previously (46,47) with minor modifications. Briefly, whole tracheas were cut into rings between two and three cartilages per ring.…”

Section: Methodsmentioning

confidence: 99%

“…In guinea pig tracheal smooth muscle, the M2 and M3 subtypes are equally present, and the M3 subtype mediates contraction (53). For these experiments, we employed cumulative concentration-response curves as commonly reported for such preparations (46,47,54). As reported (46, 47), increases in carbachol concentration led to increasing force of contraction until saturation of the response (Fig.…”

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confidence: 99%

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Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Is a Second Messenger in Muscarinic Receptor-induced Contraction of Guinea Pig Trachea

Aley

Singh

Brailoiu

et al. 2013

Journal of Biological Chemistry

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Background: NAADP is a messenger that links cell surface receptors to contraction in many cells, but its function is unknown in tracheal contraction. Results: NAADP participates in contractions induced by a neurotransmitter receptor. Conclusion: NAADP is a messenger that mediates tracheal contraction. Significance: Inappropriate tracheal contractions underlie asthma, and NAADP represents a new physiological mediator and drug target. Nicotinic acid adenine dinucleotide phosphate (NAADP) isincreasingly being demonstrated to be involved in calcium signaling in many cell types and species. Although it has been shown to play a role in smooth muscle cell contraction in several tissues, nothing is known about its possible role in tracheal smooth muscle, a muscle type that is clinically relevant to asthma. To determine whether NAADP functions as a second messenger in tracheal smooth muscle contraction, we used the criteria set out by Sutherland for a molecule to be designated a second messenger. We report that NAADP satisfies all five criteria as follows. First, the NAADP antagonist Ned-19 inhibited contractions in tracheal rings and calcium increases in isolated smooth muscle cells induced by the muscarinic agonist carbachol. Second, NAADP increased cytosolic calcium in isolated cells when microinjected and was blocked by Ned-19. Third, tracheal homogenates could synthesize NAADP by base exchange from exogenous NADP and nicotinic acid and metabolize exogenous NAADP to nicotinic acid adenine dinucleotide by a 2 -phosphatase. Fourth, carbachol induced a rapid and transient increase in endogenous NAADP levels. Fifth, tracheal homogenates contained NAADP-binding sites of high affinity. Taken together, these data demonstrate that NAADP functions as a second messenger in tracheal smooth muscle, and therefore, steps in the NAADP signaling pathway might provide possible new drug targets.Airway diameter is dynamically regulated during normal physiological processes, but inappropriate narrowing caused by hyperresponsiveness results in asthma. Airway contractility is mediated by smooth muscle contraction, which is largely driven by increases in cytosolic calcium, as in other types of smooth muscle (1-6). Smooth muscle relaxation can occur through either a decrease in calcium or an increase in cyclic AMP (7). The G-protein-coupled receptors linked to cytosolic calcium and cyclic AMP have been intensively characterized not only for a better understanding of the basic biology but also because this biological knowledge can be exploited to provide pharmacological treatments (7-11).Cytosolic calcium can increase through either release from intracellular stores or influx from the extracellular space (3,(12)(13)(14)(15)(16). Traditionally, focus has been on calcium stored in the sarcoplasmic reticulum (12, 13), which is released through channels, either inositol 1,4,5-trisphosphate (IP 3 ) 3 receptors activated by IP 3 (12, 13) or ryanodine receptors activated by cyclic . Cytosolic calcium can also increase in response to the second mess...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Is a Second Messenger in Muscarinic Receptor-induced Contraction of Guinea Pig Trachea

Aley

Singh

Brailoiu

et al. 2013

Journal of Biological Chemistry

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“…As has been reported, the contractions induced by histamine and KCl are mainly dependent on Ca 2+ from the extracelular medium, specifically an increased Ca 2+ influx across the membrane, [10] and can be eliminated by voltage-operated calcium channels blockers. [11] Considering the aforementioned, in the relaxant effect of the hexane extract a reduction of the Ca 2+ influx through calcium channels could possibly be implicated. However, further studies are needed to corroborate this idea.…”

Section: Effect Of the Hexane Extract On The Histamine Or Kcl Pre-conmentioning

confidence: 99%

Relaxant Effect of the Extracts of Crataegus mexicana on Guinea Pig Tracheal Smooth Muscle

Arrieta¹,

Siles-Barrios²,

García-Sánchez³

et al. 2010

Pharmacognosy Journal

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a B S t r a c tCrataegus sp. has been used in the traditional medicine of Mexico as well as other countries for the treatment of several respiratory diseases, such as flu, cough and asthma. The tracheal relaxant effect of the leaves of C. mexicana is investigated here for the first time, through a bioassay-guided study by using isolated tracheal rings of guinea-pig as an experimental model. the hexane extract was the most active compared to dichloromethane and methanol. an active fraction was obtained from the hexane extract. Assays by HPLC-MS reveal that at least 14 compounds may exist in it. In addition, the results suggest that relaxant effect of the effective fraction was in part related to the activity of β-adrenergic receptors and not to K + atP channels. This study represents the first in which the relaxant effect of leaves of C. mexicana on tracheal rings of guinea pig was clearly demonstrated. More studies are required to correctly identify the bioactive compounds that contribute to the relaxant effects of Crataegus mexicana, and to know the mechanisms of action of these compounds.

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Chemistry and Biology of Selected Mexican Medicinal Plants

Mata

Figueroa

Navarrete

et al. 2019

Progress in the Chemistry of Organic Natural Products

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Effect of Gnaphalium conoideum HBK on guinea pig airway smooth muscle: role of L-type Ca2+ channels

Cited by 17 publications

References 19 publications

Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Is a Second Messenger in Muscarinic Receptor-induced Contraction of Guinea Pig Trachea

Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Is a Second Messenger in Muscarinic Receptor-induced Contraction of Guinea Pig Trachea

Relaxant Effect of the Extracts of Crataegus mexicana on Guinea Pig Tracheal Smooth Muscle

Chemistry and Biology of Selected Mexican Medicinal Plants

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