Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus

Iummato, María Mercedes; Fassiano, Anabella Victoria; Graziano, Martín; Afonso, Marı́a dos Santos; Molina, Marı́a del Carmen; Juárez, Ángela Beatriz

doi:10.1016/j.ecoenv.2019.01.083

Cited by 42 publications

(29 citation statements)

References 65 publications

(85 reference statements)

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“…Interestingly, the ROS levels of algae were enhanced by high salinity and glyphosate exposure. A similar phenomenon was reported by Iummato et al (2019), indicating that the decreased activities of SOD and CAT in glyphosate‐treated M. aeruginosa could be explained by the enhancement of ROS.…”

Section: Discussionsupporting

confidence: 86%

“…In the experiment I group, the activities of SOD and CAT decreased in the L5, L10, L20, M0.5, and M0.8 treatments but were promoted in the M1.2, H20, H25, and H30 treatment groups compared with those in control-I, indicating that high concentration of glyphosate could induce oxidative stress and cause an antioxidant response in M. aeruginosa. The SOD and CAT levels were significantly decreased in green algae S. vacuolatus cells at glyphosate concentrations below 8 mg L -1 (Iummato et al 2019), which was consistent with the decreased SOD and CAT of M. aeruginosa in the L5, L10, L20, M0.5, and M0.8 treatments. The decreased activities of SOD and CAT in the M1.2, H20, H25, and H30 treatments may have been caused by the high glyphosate concentrations and its associated toxicity.…”

Section: Antioxidant Response Of M Aeruginosa To Glyphosatesupporting

confidence: 77%

“…For the determination of SOD and catalase (CAT) activity, samples were collected in 50‐mL tubes, and then washed with 20 mL phosphate buffer (pH 7.0). The intracellular components of cyanobacterial cells were extracted using a regulated method (Iummato et al 2019). Briefly, the cells were then disrupted on ice for 10 min at 300 W using an ultrasonic cell pulveriser (1200‐98; BioSafer) and then centrifuged at 5000 g for 15 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Exposure to this compound led to a 4‐fold decrease in the median effect concentration in the macrophyte Lemna minor and the green alga Pseudokirchneriella subcapitata (Cedergreen and Streibig 2005) . Many studies have reported that glyphosate increases or inhibits the growth rates of microalgae and cyanobacteria, such as Scenedesmus vacuolatus (Iummato et al 2019), Microcystis aeruginosa (Wu et al 2014), and Chlorella kessleri (Ostera et al 2020). The toxicity of glyphosate to algae is generally concentration dependent.…”

Section: Introductionmentioning

confidence: 99%

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Glyphosate Accelerates the Proliferation of Microcystis aeruginosa, a Dominant Species in Cyanobacterial Blooms

Wang

Jiang

Sheng

2020

Enviro Toxic and Chemistry

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Glyphosate is a commonly used herbicide known for its high performance in killing certain plants and grasses; however, its use is regulated due to its harmful effects on the aquatic environment. The present study investigated and compared the toxic mechanisms of glyphosate on Microcystis aeruginosa (a toxin‐producing cyanobacterium) under 2 conditions: 0‰ saline media (experiment I) and 2.5‰ saline media (experiment II). The results indicated that an appropriate concentration of glyphosate provided a phosphate source for M. aeruginosa, resulting in an increased specific growth rate in both experimental groups compared with the controls. Glyphosate‐enhanced alkaline phosphatase (ALP) activity increased by up to 1.37‐fold in experiment I and 1.68‐fold in experiment II. Moreover, the activities of superoxide dismutase (SOD) and catalase (CAT) decreased at glyphosate concentrations below 1.2 mg L–1 but increased at concentrations greater than 1.2 mg L–1 in experiment I, whereas SOD and CAT activities decreased in experiment II and declined by 64 and 49% in the 30 mg L−1 treatments. Furthermore, the transcript abundances of the pyruvate carboxylase (pcB), microcystin synthetase B (mcyB), and paired‐like homeobox (phoX) genes were up‐regulated by up to 6.92‐, 3.63‐, and 2.27‐fold in experiment I and 6.74‐, 6.55‐, and 4.86‐fold in experiment II after 96 h of incubation. The addition of glyphosate stimulated the production of dissolved organic matter including tryptophan‐like substances, fulvic acid‐like substances, (marine) humic acid‐like substances, and microcystin–leucine‐arginine in the culture. In conclusion, glyphosate stimulates the proliferation of M. aeruginosa and enhances the release of dissolved organic matter in saltwater ecosystems. Environ Toxicol Chem 2021;40:342–351. © 2020 SETAC

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Section: Discussionsupporting

confidence: 86%

Section: Antioxidant Response Of M Aeruginosa To Glyphosatesupporting

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Glyphosate Accelerates the Proliferation of Microcystis aeruginosa, a Dominant Species in Cyanobacterial Blooms

Wang

Jiang

Sheng

2020

Enviro Toxic and Chemistry

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“…Glyphosate (N-(phosphonomethyl)glycine), the world most widely applied herbicide in agriculture and urban areas [ 1 ], was first synthesized by the Swiss chemist Henri Martin in 1950 [ 2 ] as a potential pharmaceutical compound [ 3 ]. A phosphonomethyl derivative of the amino acid glycine ( Figure 1 ) [ 4 ], glyphosate is very polar, highly soluble in water (12 g/L or 71 mM at 25 °C) and insoluble in non-polar organic solvents (acetone, ethanol, and xylene) [ 5 , 6 , 7 , 8 , 9 ]. Depending on pH, glyphosate forms cationic and anionic sites within its structure [ 10 ], having a zwitterionic behavior from pH 1 to 10 [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Based Nanocomposites for Glyphosate Detection Using Surface Plasmon Resonance Sensor

Dubreuil

Peydecastaing

Vaca-Medina

et al. 2020

Sensors

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This article describes an optical method based on the association of surface plasmon resonance (SPR) with chitosan (CS) film and its nanocomposites, including zinc oxide (ZnO) or graphene oxide (GO) for glyphosate detection. CS and CS/ZnO or CS/GO thin films were deposited on an Au chip using the spin coating technique. The characterization, morphology, and composition of these films were performed by Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle technique. Sensor preparation conditions including the cross-linking and mobile phase (pH and salinity) were investigated and thoroughly optimized. Results showed that the CS/ZnO thin-film composite provides the highest sensitivity for glyphosate sensing with a low detection limit of 8 nM and with high reproducibility. From the Langmuir-type adsorption model and the effect of ionic strength, the adsorption mechanisms of glyphosate could be controlled by electrostatic and steric interaction with possible formation of 1:1 outer-sphere surface complexes. The selectivity of the optical method was investigated with respect to the sorption of glyphosate metabolite (aminomethylphosphonic acid) (AMPA), glufosinate, and one of the glufonisate metabolites (3-methyl-phosphinico-propionic acid) (MPPA). Results showed that the SPR sensor offers a very good selectivity for glyphosate, but the competition of other molecules could still occur in aqueous systems.

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Glyphosate metabolism in Tetrahymena thermophila: A shotgun proteomic analysis approach

Manan

Roytrakul

Charoenlappanit

et al. 2023

Environmental Toxicology

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Glyphosate is one of the most widely used herbicides in the world. However, because of its overuse and resistance to degradation, high levels of glyphosate residues in the environment are reported. Therefore, this study aimed to investigate the effects of glyphosate on proteomic aspects of Tetrahymena thermophila and their uses as bioindicators of freshwater ecosystem. First, an acute toxicity test was performed to determine the median inhibition concentration (IC 50 ). The toxicity test results showed that glyphosate inhibited the growth (proliferation) of T. thermophila. The 96 h-IC 50 value of glyphosate was 171 mg L À1 . No visible changes in aggregation behavior and cell morphology were observed under glyphosate exposure. In addition, the effects of low and high dose glyphosate concentrations (77.5 mg L À1 , 171 mg L À1 ) on the proteomic changes of T. thermophila was investigated using a label-free shotgun proteomic approach. A total of 3191 proteins were identified, 2791 proteins were expressed in the control, 2651 proteins were expressed in 77.5 mg L À1 glyphosates, and 3012 proteins were expressed in 171 mg L À1 glyphosates. Under glyphosate exposure at both low and high dose glyphosate, 400 unique proteins were upregulated. The majority of these proteins was classified as proteins associated with oxidative stress response and intracellular transport indicating the shifts in the internal metabolism. Proteomics revealed that the glyphosate metabolism by T. thermophila is a multi-step process involving several enzymes, which can be divided into four phases, including modification (phase I), conjugation (phase II), transport (phase III), and degradation (phase IV). The accumulation of various biochemical reactions contributes to overall glyphosate resistance. With the proteomics approach, we have found that T. thermophila was equipped with glyphosate detoxification and degradation mechanisms.

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Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus

Cited by 42 publications

References 65 publications

Glyphosate Accelerates the Proliferation of Microcystis aeruginosa, a Dominant Species in Cyanobacterial Blooms

Glyphosate Accelerates the Proliferation of Microcystis aeruginosa, a Dominant Species in Cyanobacterial Blooms

Chitosan-Based Nanocomposites for Glyphosate Detection Using Surface Plasmon Resonance Sensor

Glyphosate metabolism in Tetrahymena thermophila: A shotgun proteomic analysis approach

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