Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing

Salmani, Hossein; Nabi, Mohammad Mahdi; Vaseghi-Dodaran, Hossein; Rahman, Bahanur; Mohammadi‐Sangcheshmeh, Abdollah; Malek, S. R. A.; Towhidi, Armin; Shahneh, Ahmad Zare; Zhandi, Mahdi

doi:10.1016/j.smallrumres.2012.12.015

Cited by 74 publications

(48 citation statements)

References 27 publications

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“…Our results are in agreement with findings of Salmani et al (2013), Akhter et al (2012), Zhandi and Ghadimi (2013) and Camara et al (2011) who reported that glutathione at high concentration may have adverse effects on the structure and quality of sperm in freezing condition. However, other literatures show that glutathione can improve the quality and fertility parameters of frozen and fresh semen (Bucak and Tekin 2007;Munsi et al 2007).…”

Section: Livesupporting

confidence: 93%

“…The reasons for this discrepancy are not clear but it seems that application of different protocols and different species can effect on lipid peroxidation. Also, it seems that lipid peroxidation is not an important factor affecting sperm quality (Salmani et al 2013).…”

Section: Livementioning

confidence: 99%

“…In our previous studies, exogenous soybean lecithin has been evaluated for its efficiency against cold shock during the freezethawing process (Sharafi et al 2009;Forouzanfar et al 2010;Emamverdi et al 2013). It has been demonstrated that lecithin has suitable protective properties for ram (de Paz et al 2010), goat (Salmani et al 2013), buffalo (Akhter et al 2012) and stallion sperm (Papa et al 2011;Nouri et al 2013) against cryo-damage during freezing process. Although, soybean lecithin reduces the difficulties of egg yolk in extenders such as contamination, standardization and agglutination (Aires et al 2003), it provides the substrate for lipid peroxidation around of sperm.…”

Section: Introductionmentioning

confidence: 99%

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Supplementation of soybean lecithin-based semen extender by antioxidants: complementary flowcytometric study on post-thawed ram spermatozoa

Sharafi

Zhandi

Sharif³

2014

Cell Tissue Bank

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The purpose of the current study was to evaluate the effects of cysteine (C) and glutathione (G) on the post-thawed ram sperm quality. Collected semen samples from four mature rams were diluted with five soybean lecithin (SL)-based extenders containing: no antioxidant (SL-0), 5 mM cysteine (SL-C5), 10 mM cysteine (SL-C10), 5 mM glutathione (SL-G5) and 10 mM glutathione (SL-G10). After freeze-thawing process, motion and velocity parameters, plasma membrane integrity and functionality, morphological abnormality, lipid peroxidation, acrosomal status, mitochondria activity, and apoptosis status of post-thawed ram spermatozoa were assessed. The results showed that SL-C10 increased the total motility and plasma membrane integrity (p < 0.05) of post-thawed ram spermatozoa (55.86 ± 1.37 and 60.57 ± 1.34 %) compared to other extenders. Progressive motility was significantly higher in SL-C10 (24.71 ± 1.13 %) compared to SL-0 (20 ± 1.13 %) and SL-G10 (15 ± 1.13 %). Mitochondrial activity was significantly higher in SL-C10 (56.83 ± 2.29 %) compared to SL-G10 (38.75 ± 2.29 %). Capacitation and acrosomal status, lipid peroxidation, and the percentage of dead spermatozoa were not affected by different extenders. The percentage of live spermatozoa was higher in SL-C10 (56.33 ± 1.35 %) compared to other extenders. Also, SL-C10 resulted in a lower percentage of apoptotic spermatozoa (14.17 ± 0.53 %) compared to other extenders. The results of this study showed that supplementation of SL-based ram semen extender with 10 mM cysteine resulted in an improved quality of post-thawed ram spermatozoa.

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Section: Livesupporting

confidence: 93%

Section: Livementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Supplementation of soybean lecithin-based semen extender by antioxidants: complementary flowcytometric study on post-thawed ram spermatozoa

Sharafi

Zhandi

Sharif³

2014

Cell Tissue Bank

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“…Salmani et al [83] have suggested the use of soyabeanlecithin which is a suitable plant based cryoprotectant for caprine sperms. The beneficial effects of soyabean-lecithin as a substitute for egg yolk during cryopreservation of goat sperm are established in the works of Jiménez-Rabadán et al, Roof et al, Salmani et al, and Vidal et al [84][85][86][87].…”

Section: Cattlementioning

confidence: 99%

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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Lipid is an important constituent of cell membrane. Membrane lipid composition of spermatozoa has been correlated to different function. Many researchers have related membrane lipid with survival success after cryopreservation or cold shock. Sperm maturation and acrosome reactions are natural phenomenon, but cryopreservation or cold shock is not. Therefore, sperm cells are not programmed for such change and undergo stress. So the change in membrane lipid composition due to cold shock or cryopreservation may be looked upon as response of spermatozoa to a certain stressed condition. A significant body of research worked on the relationship between membrane lipid and fatty acid composition and ability of cell to tolerate adverse change in temperature. However, as the approach of different research groups was different, it is very difficult to compare the changes. Studies have been done with different species, ejaculated/seminal or epididymal sperm. Lipid analyses have been done with whole cell membrane isolated by different methods. Fatty acids estimated were from whole cell, plasma membrane, head membrane, or phospholipids. The cryopreservation condition, media composition, and diluents/cryoprotectants were also different. At this onset a comprehensive review is needed to cover changes of sperm membrane lipid composition of different species under different cryopreservation conditions.

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“…These studies were based, in part, on the substitution of the vitamin E by herbal antioxidants (Motlagh et al, 2014), antioxidant enzymes such as glutathione (Salmani et al, 2013) and on combination between vitamin E and other antioxidant molecules (Hsu et al, 1998;Krishnamoorthy et al, 2007;Da Silva et al, 2009). However, in our knowledge, no study has investigated the interest of vitamin E-loaded cyclodextrins in semen extenders supplementation.…”

Section: Discussionmentioning

confidence: 99%

Effect of cyclodextrins, cholesterol and vitamin E and their complexation on cryopreserved epididymal ram semen

Benhenia

Lamara

Fatmi

et al. 2016

Small Ruminant Research

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Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing

Cited by 74 publications

References 27 publications

Supplementation of soybean lecithin-based semen extender by antioxidants: complementary flowcytometric study on post-thawed ram spermatozoa

Supplementation of soybean lecithin-based semen extender by antioxidants: complementary flowcytometric study on post-thawed ram spermatozoa

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Effect of cyclodextrins, cholesterol and vitamin E and their complexation on cryopreserved epididymal ram semen

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