Effect of glutathione depletion on apoptosis induced by thiram in Chinese hamster fibroblasts

Linking protein oxidation to environmental pollutants: Redox proteomic approaches

“…Such an analysis was used recently, for instance, in frogs (Rana ridibunda) collected from sites polluted with carbamate fungicides [77], in Chinese hamster fibroblasts treated with the fungicide thiram [78], in rat interstitial cells treated with the insecticide dimethoate [79] and in various rat tissues treated with the insecticide fenvalerate [80].…”

Molecular Mechanisms of Pesticide Toxicity

“…More recently, the InsP3 receptor (InsP3R) has also been demonstrated to be regulated by ER luminal redox status through a direct interaction with ERp44, a thioredoxin family member (Banhegyi et al, 2007). Thiram chemicals have been reported to induce GSH depletion which is paralleled by protein carbonylation, lipid peroxidation and subsequent apoptotic cell death (Grosicka et al, 2005). Carbamate derivatives such as mancozeb and pyrethroids such as cyermethrin have also been shown to induce oxidative stress, DNA damage and activation of the mitochondrial pathway of apoptosis (Calviello et al, 2006;Jin et al, 2011).…”

Dithiocarbamate Toxicity - An Appraisal

“…DTC inhibit proteosome dependent protein degradation (Wang et al, 2006(Wang et al, , 2011Lovborg et al, 2006;Daniel et al, 2007;Chou et al, 2008) and promote peptide amidation (Mains et al, 1986). Thiram increases oxidative stress and induces formation of lipid peroxides, protein carbonyls, and stimulates changes in membrane potential of cells leading to ion influx inducing cell death (Erl et al, 2000;Sook Han et al, 2003;Grosicka et al, 2005). Similarly, disulfiram also induces oxidative stress that changes mitochondrial permeability leading to mitochondrial injury (Balakirev and Zimmer, 2001).…”