Effect of glutamine on liver injuries induced by intestinal ischemia-reperfusion in rats

Hartmann, Renata Minuzzo; Licks, Francielli; Schemitt, Elizângela Gonçalves; Colares, Josieli Raskopf; Silva, Juliana da; Moura, Rosa Maria Braga Lopes de; Zabot, Gilmara Pandolfo; Fillmann, Henrique Sarubbi; Marroni, Norma Possa

doi:10.20960/nh.643

Cited by 13 publications

(12 citation statements)

References 7 publications

(7 reference statements)

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“…Sellmann et al showed that Gln contributed to a reduction of inflammatory infiltration in the livers of mice with non-alcoholic steatohepatitis induced by a liquid Western-style diet (WSD)[28]. Hartmann et al found that Gln reduced hemorrhagic spots, necrosis, and inflammatory infiltration in the liver of rats subjected to intestinal ischemia–reperfusion injury[21].…”

Section: Discussionmentioning

confidence: 99%

“…It is indispensable for nucleotide, glucose, and protein synthesis. It is a precursor of GSH and plays a key role in the immune defense of the gut mucosal barrier, participating in immunoglobulin formation[20,21]. Several studies in humans have shown that Gln modulates the expression of genes related to the activation of pathways involved in several disease states[22-25].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies in humans have shown that Gln modulates the expression of genes related to the activation of pathways involved in several disease states[22-25]. Furthermore, studies conducted in different experimental models have demonstrated beneficial effects of Gln on the rat liver, bowel, and stomach[21,26-30].…”

Section: Introductionmentioning

confidence: 99%

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Protective action of glutamine in rats with severe acute liver failure

Schemitt¹,

Hartmann²,

Colares³

et al. 2019

WJH

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BACKGROUND Severe acute liver failure (SALF) is a rare, but high-mortality, rapidly evolving syndrome that leads to hepatocyte degeneration with impaired liver function. Thioacetamide (TAA) is a known xenobiotic, which promotes the increase of the formation of reactive oxygen species. Erythroid 2-related factor 2 (Nrf2) activates the antioxidant protection of cells. Studies have evidenced the involvement of inflammatory mediators in conditions of oxidative stress. AIM To evaluate the antioxidant effects of glutamine on Nrf2 activation and NFκB-mediated inflammation in rats with TAA-induced IHAG. METHODS Male Wistar rats ( n = 28) were divided into four groups: control, control+glutamine, TAA, and TAA + glutamine. Two TAA doses (400 mg/kg) were administered intraperitoneally, 8 h apart. Glutamine (25 mg/kg) was administered at 30 min, 24 h, and 36 h. At 48 h, blood was collected for liver integrity analysis [aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP)]. The liver was harvested for histology and assessment of oxidative stress [thiobarbituric acid-reactive substances (TBARS), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione (GSH), Nrf2, Kelch-like ECH-associated protein 1 (Keap1), NADPH quinone oxidoreductase1 (NQO1), superoxide dismutase (SOD)] and inflammatory process. RESULTS TAA caused disruption of the hepatic parenchyma, with inflammatory infiltration, massive necrosis, and ballooning degeneration. Glutamine mitigated this tissue damage, with visible regeneration of hepatic parenchyma; decreased TBARS ( P < 0.001), GSH ( P < 0.01), IL-1β, IL6, and TNFα levels ( P < 0.01) in hepatic tissue; and decreased blood levels of AST, ALT, and ALP ( P < 0.05). In addition, CAT, GPx, and GST activities were restored in the glutamine group ( P < 0.01, P < 0.01, and P < 0.001, respectively vs TAA alone). Glutamine increased expression of Nrf2 ( P < 0.05), NQO1, and SOD ( P < 0.01), as well as levels of IL-10 ( P < 0.001), while decreasing expression of Keap1, TLR4, NFκB ( P < 0.001), COX-2 and iNOS, ( P < 0.01), and reducing NO 2 and NO 3 levels ( P < 0.05). CONCLUSION In the TAA experimental model of IHAG, glutamine activated the Nrf2 pathway, thus promoting antioxidant protection, and blunted the NFκB-mediated pathway, reducing inflammation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective action of glutamine in rats with severe acute liver failure

Schemitt¹,

Hartmann²,

Colares³

et al. 2019

WJH

Self Cite

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“…(3) Glutamine has important immunomodulatory effects that are required for lymphocyte secretion, proliferation, and maintenance of its function (Dos Santos et al, 2017). 4This molecule is involved in the synthesis of glutathione (GSH), which exerts strong antioxidative effect against stress damage (Hartmann et al, 2017). (5) Glutamine is an essential nutrient for the metabolism of intestinal mucosal cells and maintains the integrity of the intestinal mucosal epithelial structure (Kim and Kim, 2017a).…”

Section: The Resource and Function Of Glutaminementioning

confidence: 99%

“…Hence, antioxidative stress is an important strategy against such injury. GSH is an important endogenous antioxidant that has a prominent contribution against oxidative stress (Hartmann et al, 2017). Lian et al (2018) showed that glutamine improves the oxidative stress response induced by myocardial I/R by enhancing GSH synthesis.…”

Section: Glutamine Improves Myocardial Ischemia-reperfusion Injury Wimentioning

confidence: 99%

Essential Role of Nonessential Amino Acid Glutamine in Atherosclerotic Cardiovascular Disease

Chen

Zhang

et al. 2020

DNA and Cell Biology

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Atherosclerosis is a major disease that seriously harms human health and is known as the ''number one killer'' in developed countries and the leading cause of death worldwide. Glutamine is the most abundant nonessential amino acid in the human blood that has multifaceted effects on the body. Recent studies showed that glutamine is negatively corrected with the progression of atherosclerotic lesions. In this review, we focused on the relationship of glutamine with macrophage polarization, nitrification stress, oxidative stress injury, myocardial ischemia-reperfusion injury, and therapeutic angiogenesis to review its roles in atherosclerotic cardiovascular disease.

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Effects of Ala-Gln on growth, biochemical indicators and stress-related gene expression of largemouth bass (Micropterus salmoides) under dual stress of flow rate and density

Lin,

Chen,

Chen

et al. 2024

Aquaculture Reports

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Effect of glutamine on liver injuries induced by intestinal ischemia-reperfusion in rats

Abstract: Pretreatment with Gln reduced oxidative, tissue damage and showed a decrease expression of inflammatory mediators.

Cited by 13 publications

References 7 publications

Protective action of glutamine in rats with severe acute liver failure

Protective action of glutamine in rats with severe acute liver failure

Essential Role of Nonessential Amino Acid Glutamine in Atherosclerotic Cardiovascular Disease

Effects of Ala-Gln on growth, biochemical indicators and stress-related gene expression of largemouth bass (Micropterus salmoides) under dual stress of flow rate and density

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